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Land is an essential resource tomaintain the functioning of the socio‐economic system. Due to sectoral land data limitations, previous studies were primarily restricted to a coarse sectoral level or focused mainly on the global and national scales. However,fine‐scale land use data are required to provide tailored implications for municipal sustainable development. With emerging geographic data and novel methods, including point of interest data, road network data, and natural language processing, a bottom‐up model is developed to estimate the entity‐level artificial impervious land use. Then, we conducted a case study in Shanghai to investigate the spatial features, footprints, and intensities of sectoral land use. Our results indicated that 42 sectors in Shanghai had diverse spatial patterns. The transportation sector had the highest level of agglomeration among all sectors, and the manufacturing industry's adjacent land patches had higher sectoral heterogeneities than the service sector. The transportation sector had the largest direct and embodied land use footprint. The residential‐related sectors had higher land use intensities, while the high value‐added service sectors showed lower land use intensities. Our study indicates that this model offers a novel way of extracting entity‐level spatial land use information and is applicable for socio‐economic metabolism research. Future studies could incorporate remote sensing images and multiple databases to achieve higher resolution. [ABSTRACT FROM AUTHOR]

This paper introduces the basic situation of the 35-kV domestic high temperature superconducting (HTS) power cable project in Shanghai. The target of this project is to construct and put into operation the first 35-kV km level three-phase turn-key HTS power cable project in the world. The cable transmission capacity, cable routing selection, cable structure design, cooling system configuration, etc. of the project are introduced. The project is implemented by the State Grid Corporation Shanghai Electric Power Company. A 35-kV, 1.2-km-long 220-kV inter-substation connecting HTS cable line will be constructed in the core urban area of Shanghai. So far, the type test of the HTS power cable system has been completed, and the construction of the project has begun. The project is expected to be completed and put into operation in the second half of 2020. [ABSTRACT FROM AUTHOR]

Objective: To explore the prevalence of and risk factors for gallstone disease in Shanghai, China. Methods: A population‐based cross‐sectional study was conducted in Shanghai between 2016 and 2017. Using a three‐stage stratified sampling strategy, 4009 participants (1753 men and 2256 women) from 10 districts were enrolled. Results: The overall prevalence of gallstones was 6.83% (6.22% for men vs 7.31% for women, P = 0.173). According to the multivariate analysis, individuals aged ≥40 years (odds ratio [OR] 3.058, 95% confidence interval [CI] 2.110–4.433, P < 0.001), hypertension (OR 1.479, 95% CI 1.076–2.034, P = 0.016), thyroid disease (OR 1.409, 95% CI 1.029–1.928, P = 0.032), a family history of gallstones (OR 2.234, 95% CI 1.362–3.662, P = 0.001) and a waist‐to‐height ratio ≥0.5 (OR 1.656, 95% CI 1.197–2.292, P = 0.002) had an increased risk of developing gallstones. The risk of gallstone disease was 2.232 (95% CI 1.167–4.268, P = 0.015) times higher in individuals with elevated C4 levels than in those with normal C4 levels. Diabetes (OR 4.144, 95% CI 1.171–14.671, P = 0.028) was a risk factor for the formation of gallstones with diameters ≥1 cm, and men were more susceptible to develop multiple stones (OR 2.356, 95% CI 1.321–4.200, P = 0.004). Conclusion: Individuals aged ≥40 years, with a history of hypertension and familial gallstones, a high waist‐to‐height ratio, thyroid disease and high C4 levels were related to an increased risk of gallstone disease. [ABSTRACT FROM AUTHOR]

Study Question: Are there any differences between in vivo (IVV) and in vitro (IVT) matured metaphase II (MII) oocytes at the molecular level?Summary Answer: Between IVV and IVT oocytes, 507 differentially expressed genes (DEGs) were identified; the non-CpG methylomes were significantly different, but the CpG methylomes and genomic copy number variations (CNVs) were similar.What Is Known Already: A previous study using microarray and single-cell RNA-seq analysis revealed that numerous genes were differentially expressed between IVV and IVT oocytes. Independent studies of DNA methylation profiling in human oocytes have revealed negative correlations between gene transcription and the DNA methylation level at gene promoter regions. No study has compared global CpG or non-CpG methylation between these two groups of oocytes. Although a high level of aneuploidy has been reported in MII oocytes, no direct comparison of IVV and IVT oocytes based on single-cell sequencing data has been performed.Study Design, Size, Duration: We collected eight IVV oocytes from six patients and seven IVT oocytes from seven patients and then analysed each oocyte using the previously established single-cell triple omics sequencing (scTrioseq) analysis to determine associations among the transcriptome, DNA methylome and chromosome ploidy in the oocytes.Participants/materials, Setting, Methods: All IVV oocytes were donated by patients who received 150 IU gonadotropin per day from the third day of their menstrual cycle, followed by GnRH antagonist after 5 days of gonadotropin stimulation. All IVT oocytes were from immature oocytes which were donated by volunteers undergoing delivery by caesarean section then cultured in oocyte maturation medium containing 75 mIU/ml hMG for 24 to 48 h. Every single oocyte was analysed using the previously established single-cell multiomic sequencing analysis.Main Results and the Role Of Chance: There were 507 genes differentially expressed between the IVV (n = 8) and IVT (n = 7) oocytes, even though their global transcriptome profiles were similar. The enriched genes in IVV oocytes were related to the cell cycle process while those in IVT oocytes were related to mitochondrial respiration biogenesis. Although the global CpG methylation of the two groups of oocytes was similar, the non-CpG methylation level in IVV oocytes was higher than that in IVT oocytes. A high aneuploidy ratio was found in both groups, but the aneuploidy did not affect transcription according to the correlation analysis.Large-scale Data: N/A.Limitations and Reasons For Caution: Due to the difficulty in collecting MII oocytes, especially IVV matured oocytes, the sample size was limited.Wider Implications Of the Findings: Our findings indicate that single-cell multiomic sequencing can be utilised to examine the similarity and differences between IVV and IVT matured MII oocytes.Study Funding/competing Interest(s): This work was supported by the Ministry of Science and Technology of China, National Key R&D Program of China (No. 2017YFC1001601). The donated oocytes were collected by Shanghai Tenth People's Hospital. The authors declare no competing interests. [ABSTRACT FROM AUTHOR]

A correction to this paper has been published: https://doi.org/10.1007/s10948-021-05931-w [ABSTRACT FROM AUTHOR]