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Empowering teachers through sharing communal decision-making responsibility via distributed leadership has been shown to be effective for positive change in schools. While studies have proposed various psychosocial channels through which positive effects on teacher wellbeing can be realized, there is scarce evidence on how this relationship is influenced by teacher self-efficacy. This study examines how self-efficacy mediates the relationship between distributed leadership, job and career wellbeing among secondary school teachers, employing a partial least-squares structural equation model using the Teaching and Learning International Survey (TALIS) Shanghai dataset (N = 3799). Results show that distributed leadership is positively associated with improvement in self-efficacy (std. β = 0.33, P < 0.001), job wellbeing (std. β = 0.51, P < 0.001), and career wellbeing (std. β = 0.45, P < 0.001), whereas self-efficacy is positively correlated with job wellbeing (std. β = 0.15, P < 0.001), but not career wellbeing (std. β = −0.01, P = 0.69). In terms of mediation effects, self-efficacy positively mediates the relationship between distributed leadership and job wellbeing (std. β = 0.05, P < 0.001), but distributed leadership does not indirectly influence career wellbeing (std. β = −0.002, P = 0.70) via channels through self-efficacy. [ABSTRACT FROM AUTHOR]

The progression from non-muscle-invasive bladder cancer (NMIBC) to muscle-invasive bladder cancer (MIBC) increases the risk of death. It is therefore important to find new relevant molecular models that will allow for effective prediction of the progression and prognosis of bladder cancer (BC). Using RNA-Sequence data of 49 BC patients in Shanghai tenth people's hospital (STPH) and weighted gene co-expression network analysis methods, a co-expression network of genes was developed and three key modules associated with malignant progression were selected. Based on the genes in three key modules, an eight-gene risk signature was established using univariate Cox regression and the Least absolute shrinkage and selection operator Cox model in The Cancer Genome Atlas Program (TCGA) and validated in validation sets. Subsequently, a nomogram based on the risk signature was constructed for prognostic prediction. The mRNA and protein expression levels of eight genes in cell lines and tissues were further investigated. The novel eight-gene risk signature was closely related to the malignant clinical features of BC and could predict the prognosis of patients in the training dataset (TCGA) and four validation sets (GSE32894, GSE13507, IMvigor210 trial, and STPH). The nomogram showed good prognostic prediction and calibration. The mRNA and protein expression levels of the eight genes were differentially expressed in cell lines and tissues. In our study, we established a novel eight-gene risk signature that could predict the progression and prognoses of BC patients. [ABSTRACT FROM AUTHOR]

Aimaitiaji Kadier,* Ruiliang Wang, Haimin Zhang, Xudong Yao Department of Urology, Shanghai Tenth People's Hospital, Tongji University of Medicine, Shanghai, 200072, People's Republic of China*These authors contributed equally to this workCorrespondence: Xudong Yao; Haimin ZhangDepartment of Urology, Shanghai Tenth People's Hospital, Tongji University of Medicine, Shanghai 200072, People's Republic of ChinaEmail yaoxudong1967@163.com; zhm1064@163.com Objective: NUSAPl and O-GlcNAcylation were reported to be hyper-activated in many kinds of cancers and involved in the advanced progression of cancers. In bladder cancer, O-GlcNAc transferase (OGT) expresses in patients' urine samples, with no expression in healthy individuals, indicating O-GlcNAcylation might involve in the occurrence and development of bladder cancer. Therefore, the present study aims to investigate the effects of O-GlcNAcylation in bladder cancer and if it can regulate NUSAP1 protein. Materials and Methods: Western blot, immunohistochemistry, and PCR were used to evaluate the protein expression and mRNA level of NUSAP1; CCK-8 and flow cytometry used to evaluate the proliferation and inhibited the apoptosis of bladder cancer. Results: The results showed that NUSAP1 was highly expressed in bladder cancer cells and tissue samples. NUSAP1 up-regulation significantly promoted the proliferation and inhibited the apoptosis of bladder cancer HT-1376 and T24 cells. Besides, the expression of O-GlcNAc was elevated in bladder cancer tissues and cells, and up-regulation of O-GlcNAc with GlcNAc and PuGNAc obviously increased NUSAP1 protein expression and stability. Moreover, knockdown OGT significantly inhibited the proliferation and tumorigenesis and promoted the apoptosis of bladder cancer cells, confirmed by CCK-8, in vivo xenotransplantation, and flow cytometry, whereas these roles were impaired when NUSAP1 was up-regulated. Conclusion: Overall, our study makes clear that hyper-O-GlcNAcylation accelerates bladder cancer progression through promotion of NUSAP1 expression and its stability. [ABSTRACT FROM AUTHOR]