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To explore the applicability of the carbon isotope composition (δ13C) of fossil charcoal for the quantitative reconstruction of paleoclimates, we selected five points in Shaanxi province, from north to south, to collect modern Pinus species and Quercus species to sample covering areas with obvious climatic differences. In order to reveal the relationships between δ13C of charcoal and climate variables on the basis of carbonization experiments, we evaluated the fractionation mechanism of δ13C of charcoal, and compared the differences between δ13C of charcoal in wildfire experiments and indoor experiments regarding genera and species. The results showed significant differences in δ13C between genera but no significant differences among species. Additionally, the δ13C of charcoal was significantly negatively correlated with precipitation and positively correlated with evaporation, which could be determined from δ13C values in the study area to reconstruct ancient precipitation and evaporation in the future. [ABSTRACT FROM AUTHOR]