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Transcranial direct current stimulation (tDCS) is known to modulate the brain excitability and enhance cognitive functions and neuroplasticity, although adherence to its administration in post-stroke rehabilitation is still being understudied. This study set out to develop and content validate a questionnaire to assess clinical physiotherapists’ knowledge, attitude, and practices regarding the use of tDCS following a stroke. In order to gather expert opinion and come to a consensus on a certain topic, the Delphi technique was employed. The measure was validated using both qualitative (cognitive interviewing) and quantitative (content validity) methods on a panel of 32 experts. Calculations were made for the content validity ratio (CVR), content validity index (CVI), item-level CVI (I-CVI), and scale-level CVI universal agreement (S-CVI/UA). A total of 48 interdisciplinary experts in the field of neurosciences were invited. In all, 32 specialists from Neurology, General Medicine, Neurophysiotherapy, and Physiology departments accepted the invitation and provided their opinion for instrument analysis. After two rounds, early iterations of this instrument demonstrated an acceptable CVR value of 1, high overall content validity (S-CVI/UA = 0.86), and high content validity of individual items (I-CVI range: 0.83-1.00). The kappa value varied between 0.75 and 1, which is excellent. Its content is therefore deemed validated. Through an iterative process, its development and assessment revealed strong item-content validity for determining the domains of the questionnaire. It is anticipated that this metric could be utilized to increase the adherence rate of post-stroke tDCS application in Saudi Arabia. [ABSTRACT FROM AUTHOR]

This study examines gender differences of teachers on their mathematical knowledge for teaching in the context of single-sex classrooms in Saudi Arabia. A translated version of the Mathematical Knowledge for Teaching (MKT) instrument (Learning Mathematics for Teaching [LMT], 2008) in Number and Operation Content Knowledge (CK) and Knowledge of Content and Student (KCS) scales were administered to 197 teachers (146 male and 51 female). Two-sample "t" test and multiple regression were conducted to compare the two groups and test the effect of teacher background variables. Female teachers significantly scored better than their male counterpart. Gender, years of teaching experience, and specialization significantly predicted teachers' content knowledge, F(3, 187) = 13.180, explaining 41.8% of the variance. Only gender and specialization significantly predicted teachers' knowledge of content and student, F(2, 191) = 6.335, explaining 24.9% of the variance. Further comparing items in the MKT instrument where female teachers outperformed male teachers confirmed that female teachers were better in attending to the content knowledge in the context of student's learning.

Basaltic lavas from Harrat Uwayrid, Saudi Arabia, record the evolving magmatic and tectonic context of the Arabian Peninsula from at least the mid‐Miocene to the present day. New 40Ar/39Ar ages spanning from the mid to late Miocene reveal that mid‐Miocene mafic volcanism formed a large, subalkaline volcanic plateau parallel to Red Sea rifts. Subsequent volumetrically subordinate late Miocene‐Quaternary alkaline volcanism erupted monogenetic cinder cones roughly orthogonal to the earlier volcanic field. The source region for all samples was affected by both fluid and silicate metasomatism; inferred mantle mineral assemblages include amphibole for mid‐Miocene lavas and phlogopite for late Miocene‐Quaternary samples. Calculated melting depths become shallower with time across the Miocene volcanic episode (∼20–15 Ma) but become deeper in the late Miocene to Quaternary (∼10–0 Ma), indicating melting pressures and temperatures significantly higher than those recorded in Miocene lavas despite progressive lithospheric thinning. We offer a two‐stage model for the formation of Harrat Uwayrid: (a) Early‐ and mid‐Miocene rifting associated with the Red Sea opening facilitated adiabatic melting of uppermost mantle lithosphere to form the early volcanic plateau and (b) Plate motion changes in the mid‐ and late‐Miocene initiated the Dead Sea Fault and destabilized a dense pyroxenitic lower lithosphere leading to foundering or lithospheric drip beneath Harrat Uwayrid that allowed deep lithospheric melting and formed the young volatile‐rich eruptives. Key Points: Mafic volcanism at Harrat Uwayrid records the geodynamic evolution of the Arabian plate margin since the OligoceneMio‐Pliocene lavas formed from adiabatic melting as a result of lithospheric extension associated with spreading of the Red SeaVolatile‐rich Quaternary alkaline basalts reflect melting of hydrated metasomatized lithosphere [ABSTRACT FROM AUTHOR]

Background: Community pharmacists' interventions have been found to be highly effective. However, to date, there has been little information about pharmacists' potential roles and perceived barriers to providing smoking cessation. This study aimed to assess the potential role of community pharmacists in supporting their patients to quit smoking by summarizing their self-reported level of current activities, confidence, and perceived barriers; Methods: A self-administered questionnaire was used for a cross-sectional study in Saudi Arabia. As part of the questionnaire, demographic characteristics were measured, as well as confidence, perceived berries, and level of smoking cessation activities (asking, advising, assessing, assisting, and arranging, including follow-up).; Results: 370 community pharmacists practicing responded to the survey. Pharmacists indicated high activity rates about advising and assessing patients in quitting smoking, with lower rates of assisting and arranging, including following up. The rate of recording smoking status was very low. There were significant differences between gender, source of education, years practicing as a licensed pharmacist, attended an education or training program on smoking cessation, and interest in providing smoking and perceived practice of smoking cessation. Pharmacists are confident about providing smoking cessation activities. Barriers to providing smoking cessation services include unable to follow up, lack of counseling space, lack of educational materials, and lack of time.; Conclusions: Saudi Arabian community pharmacists actively offer smoking cessation services, which may reduce smoking-related health issues. It appears that pharmacists need to be empowered by team-based, systematic, comprehensive approaches to overcome barriers and enhance their confidence. [ABSTRACT FROM AUTHOR]

Chromosomal imbalance is implicated in developmental delay (DD), congenital malformations (CM), and intellectual disability (ID), and, thus, precise identification of copy number variations (CNVs) is essential. We therefore aimed to investigate the genetic heterogeneity in Saudi children with DD/CM/ID. High-resolution array comparative genomic hybridization (array CGH) was used to detect disease-associated CNVs in 63 patients. Quantitative PCR was done to confirm the detected CNVs. Giemsa banding-based karyotyping was also performed. Array CGH identified chromosomal abnormalities in 24 patients; distinct pathogenic and/or variants of uncertain significance CNVs were found in 19 patients, and aneuploidy was found in 5 patients including 47,XXY (n = 2), 45,X (n = 2) and a patient with trisomy 18 who carried a balanced Robertsonian translocation. CNVs including 9p24p13, 16p13p11, 18p11 had gains/duplications and CNVs, including 3p23p14, 10q26, 11p15, 11q24q25, 13q21.1q32.1, 16p13.3p11.2, and 20q11.1q13.2, had losses/deletions only, while CNVs including 8q24, 11q12, 15q25q26, 16q21q23, and 22q11q13 were found with both gains or losses in different individuals. In contrast, standard karyotyping detected chromosomal abnormalities in ten patients. The diagnosis rate of array CGH (28%, 18/63 patients) was around two-fold higher than that of conventional karyotyping (15.87%, 10/63 patients). We herein report, for the first time, the extremely rare pathogenic CNVs in Saudi children with DD/CM/ID. The reported prevalence of CNVs in Saudi Arabia adds value to clinical cytogenetics. [ABSTRACT FROM AUTHOR]

Urinary tract infections (UTIs) are one of the most common long-term complications of diabetes mellitus (DM). Additionally, various factors, such as socio-demographics, type of DM, fasting blood glucose, regular diabetes monitoring, comorbid chronic diseases, HbA1c, body mass index (BMI), and duration of DM, are also thought to predispose individuals to developing UTIs more frequently when they have DM. This research aims to evaluate the risk factors for UTIs and their prevalence among people with DM in Saudi Arabia (KSA). This cross-sectional study was conducted among 440 adults with type 1, type 2, and gestational DM. The participants had to be at least 18 years old, of both genders, and had been suffering from DM for any period of time. A self-administered questionnaire was utilized to collect data on demographic characteristics, such as sex, age, height, weight, material state, education level, income, and clinical profiles of DM and UTI. The crude (COR) and adjusted odds ratios (AOR) were calculated using logistic regression in the IBM SPSS software. The incidence of types 1 and 2 DM and gestational diabetes reached 34.1, 60.9, and 5%, respectively. Most of the participants had first-degree relatives with DM (65.9%). UTI was common in 39.3% of participants. A chi-squared statistical analysis revealed that the frequency of UTI varied depending (χ2 = 5.176, P = 0.023) on the type of DM. Burning urination and abdominal pain were the most common symptoms. The CORs for sex, marital status, hypertension, and BMI were significant (P < 0.05) and had values of 2.68 (95% CI = 1.78–4.02), 0.57 (95% CI = 0.36–0.92), 1.97 (95% CI = 1.14–3.43), and 2.83 (95% CI = 1.19–2.99), respectively. According to the adjusted model, only sex influenced the occurrence of UTIs. The AOR for sex was 3.45 (95% CI = 2.08–5.69). Based on this study, the authorities related to the health of DM patients can use its findings to guide awareness programs and clinical preparedness. [ABSTRACT FROM AUTHOR]

Simple Summary: Interspecies SCNT-based cloning and in vitro production of interspecies SCNT-derived embryos allows cells that have undergone terminal differentiation to be reprogrammed to become totipotent cells. The development of the interspecific Somatic cell nuclear transfer oryx in vitro for the first time in Saudi Arabia will promote additional research on animal reproductive cloning for the preservation of endangered species. Cloning, commonly referred to as somatic cell nuclear transfer (SCNT), is the technique of enucleating an oocyte and injecting a somatic cell into it. This study was carried out with interspecific SCNT technology to clone the Arabian Oryx utilizing the oryx's fibroblast cells and transfer it to the enucleated oocytes of a domestic cow. The recipient oocytes were extracted from the cows that had been butchered. Oryx somatic nuclei were introduced into cow oocytes to produce embryonic cells. The study was conducted on three groups, Oryx interspecific somatic cell nuclear transfer into enucleated oocytes of domestic cows, cow SCNT "the same bovine family species", used as a control group, and in vitro fertilized (IVF) cows to verify all media used in this work. The rates of different embryo developmental stages varied slightly (from 1- cell to morula stage). Additionally, the oryx interspecies Somatic cell nuclear transfer blastocyst developmental rate (9.23%) was comparable to that of cow SCNT (8.33%). While the blastula stage rate of the (IVF) cow embryos exhibited a higher cleavage rate (42%) in the embryo development stage. The results of this study enhanced domestic cow oocytes' ability to support interspecific SCNT cloned oryx, and generate a viable embryo that can advance to the blastula stage. [ABSTRACT FROM AUTHOR]

Cancer is one of the major causes of death worldwide. The repercussions of conventional therapeutic approaches present a challenge in the delivery of new effective treatments. Thus, more attention is being awarded to natural products, mainly honey. Honey could be the basis for the development of new therapies for cancer patients. The aim of this study is to assess the phytochemical profiling, antioxidant, drug-likeness properties, and anticancer activity of Ziziphus honey (ZH) derived from the Hail region of Saudi Arabia. The phytochemical profiling using high resolution-liquid chromatography mass spectrometry (HR-LCMS) revealed 10 compounds belonging to several familial classes and one tripeptide. Potential antioxidant activity was noted as assessed by DPPH (IC50 0.670 mg/mL), ABTS (IC50 3.554 mg/mL), and β-carotene (IC50 > 5 mg/mL). The ZH exerted a notable cytotoxic effect in a dose-dependent manner against three cancer cell lines: lung (A549), breast (MCF-7), and colon (HCT-116), with respective IC50 values of 5.203%, 6.02%, and 7.257%. The drug-likeness investigation unveiled that most of the identified compounds meet Lipinski's rule. The molecular docking analysis revealed interesting antioxidant and anticancer activities for most targeted proteins and supported the in vitro findings. The Miraxanthin-III compound exhibited the most stabilized interaction. This study provides deeper insights on ZH as prominent source of bioactive compounds with potent antioxidant and anticancer effects. [ABSTRACT FROM AUTHOR]

With the increasing global concerns about greenhouse gas emissions caused by the extensive use of fossil fuels, many countries are investing in the deployment of clean energy sources. The utilization of abundant solar energy is one of the fastest growing deployed renewable sources due its technological maturity and economic competitivity. In addition to the report from the National Renewable Energy Laboratory (NREL), many studies have suggested that the maturity of solar energy systems will continue to develop, which will increase their economic viability. The focus of analysis in this paper is countries with hot desert climates since they are the best candidates for solar energy systems. The capital of Saudi Arabia, Riyadh, is used as the case study due to the country’s ambitious goals in this field. The main purpose of this study is to comprehensively analyze the stochastic behavior and probabilistic distribution of solar irradiance in order to accurately estimate the expected power output of solar systems. A solar photovoltaic (PV) module is used for the analysis due to its practicality and widespread use in utility-scale projects. In addition to the use of a break-even analysis to estimate the economic viability of solar PV systems in hot desert climates, this paper estimates the indifference point at which the economic feasibility of solar PV systems is justified, compared with the fossil-based systems. The numerical results show that the break-even point of installing one KW generation capacity of a solar PV system is estimated to pay off after producing 16,827 KWh, compared to 15,422 KWh for the case of fossil-based systems. However, the increased cost of initial investment in solar PV systems deployment starts to be economically justified after producing 41,437 KWh. [ABSTRACT FROM AUTHOR]

Several newly nonlinear models for describing dynamics of COVID-19 pandemic have been proposed and investigated in literature recently. In light of these models, we attempt to reveal the role of fractional calculus in describing the growth of COVID-19 dynamics implemented on Saudi Arabia's society over 107 days; from 17 Dec 2020 to 31 March 2021. Above is achieved by operating two fractional-order differential operators, Caputo and the Caputo-Fabrizio operators, instead of the classical one. One of expanded SEIR models is utilized for achieving our purpose. With the help of using the Generalized Euler Method (GEM) and Adams-Bashforth Method (ABM), the numerical simulations are performed respectively in view of the Caputo and Caputo-Fabrizio operators. Accordance with said, the stability analysis of the two proposed fractional-order models is discussed and explored in view of obtaining the equilibrium points, determining the reproductive number (R0) and computing the elasticity indices of R0. Several numerical comparisons reveal that the fractional-order COVID-19 models proposed in this work are better than that of classical one when such comparisons are performed between them and some real data collected from Saudi Arabia's society. This inference together with the cases predictions that could easily deduced from the proposed fractional-order models can allow primary decision makers and influencers to set the right plans and logic strategies that should be followed to face this pandemic. [ABSTRACT FROM AUTHOR]

Introduction: Preventive oral health of a child is dependent on the parental knowledge toward caries control, care of deciduous tooth, use of space maintainers for malocclusion, and utilization of various procedures available for preventing oral diseases by oral health professionals. This study was aimed to assess the attitude and awareness of preventive dentistry among parents residing in Riyadh city, Kingdom of Saudi Arabia. Methodology: A cross-sectional, questionnaire study was conducted among 300 male parents. Questionnaire was sent through the use of social media, and responses were collected. Results: Seventy-four percent of male Saudi parents were aware of prevention of oral diseases. 38.45% of male parents were aware of preventive dentistry procedure, but preventive dentistry utilization rate was seen among 6% population only. Attitude toward the prevention of oral disease was statistically not significant according to the age group and income but statistically significant according to education. The utilization of preventive procedures was significant according to education and income (P < 0.05). Conclusion: Awareness about preventive dentistry was more among Saudi parents but utilization rate was directly correlated to their education and income. After The utilization of preventive procedures was significant according to education and income (P < 0.05). [ABSTRACT FROM AUTHOR]

Background and Objective. Anxiety is an emotional and behavioral disorder that may disturb the student's quality of learning and its outcome. This study is aimed at assessing the prevalence and associated factors among pharmacy students at a university in Saudi Arabia. Methods. We used a cross-sectional design, and data collection was carried out over a period of two months from September 2018 to November 2018 using paper-based self-administered questionnaires. The General Anxiety Disorder-7 (GAD-7) scale was used to measure and classify anxiety among the study participants. Results. The prevalence of anxiety among pharmacy students was 49% (83 students); 44 students (25.9%) had mild anxiety, while 24 (14.1%) students had moderate anxiety, and 15 (8.8%) severe anxiety. There were statistically significant differences in anxiety scores according to faculty type (p = 0.2) and nutritional status (p = 0.4). Conclusion. The findings of this study revealed that half of the pharmacy students suffered from anxiety incidence during their studies at the university. However, the majority of them are experiencing mild to moderate. This may have a significant impact on academic performance and necessitates special attention. [ABSTRACT FROM AUTHOR]

Purpose: Dietary supplements (DSs) are popular in many countries, and their use among individuals is increasing worldwide. Therefore, this study aimed to assess the prevalence and use of DSs among pharmacy students in King Saud University College of Pharmacy, Riyadh, Saudi Arabia. Methods: This study used a cross-sectional design targeting male senior pharmacy students in their fourth and fifth years of Bachelor and Doctor of Pharmacy courses. The data were collected between August and October 2019 using paper-based questionnaires. Results: A total of 46.8% of the students used DSs. Of all students surveyed, 19% used branded supplements whereas 9.7% used generic or local supplements and 12.3% used both generic and branded supplements. Furthermore, 8.2% students suffered from side effects, including 5.6% who suffered from nausea, vomiting, and diarrhea and 2.6% who suffered from headache, confusion, and disorientation. Approximately 24.6% of students used fiber DSs whereas 19% and 16.4% used DSs for protein and glucosamine/omega 3 fatty acids, respectively. Conclusion: The study findings indicated that the prevalence of DS use is increasing. However, approximately half of the respondents encourage the use of DSs only with a doctor's recommendation. Educating about the safe use of DSs is warranted. [ABSTRACT FROM AUTHOR]

Purpose. The objective of the current study was to determine the prevalence and practice of unused and expired medicine among Saudi adults. Subjects and Methods. The study used cross-sectional web-based design to collect the data, over a period of 4 months among people who are living in Riyadh, Saudi Arabia. Results. A total of 337 questionnaires were obtained during the study period. The majority of respondents were university graduates n = 251 (74.7%). The prevalence of unused medicine was n = 301 (89.3%). The most commonly used drugs were nonsteroidal anti-inflammatory drugs n = 272 (80.7%) and antibiotics n = 164 (48.7%). Of the participants, 186 (55.2%) checked the expiry date of the medicine before they purchase. Most of them n = 305 (90.5%) obtained medicine through prescription. About n = 219 (65%) of them keep the medicine until it expired; 48.1% throw away in the household garbage while only 18 (5.4%) of the respondents said to give it back to the medical store. Conclusion. The study revealed a high prevalence of unused medications among Saudi community. However, the disposable practice among the Saudi community was inadequate. Increasing awareness through education programs about proper disposable guidelines is necessary for controlling the medication wastage. [ABSTRACT FROM AUTHOR]

Background: chronic kidney disease (CKD) is common in Saudi although there are few data on the prevalence of this disorder. Therefore, we initiated a multicenter screening study to identify the prevalence and staging of CKD in 712 patients with known hypertension in four hospitals in Riyadh, Saudi Arabia. Method: we measured estimated glomerular filtration rate by the six-variable modification of diet in renal disease equation and proteinuria by the protein/creatinine ratio. All the subjects studied were Saudis. Results: of the 712 patients studied, the median age was 59 years (range 19-90 years) and 560 (78.7%) of the patients were female. The mean duration of hypertension was 4 years (range 0.1-50). The overall prevalence of CKD was 46.9% (95% CI: 43.2-50.7%),19.1% had CKD stages 1-2 and 27.8% had CKD stages 3-5. There was no difference in age between patients with or without CKD (p = 0.12). The overall prevalence of proteinuria was 28.9% (95% CI: 25.6-32.4%),14.7% of subjects had preexisting diabetes mellitus and their prevalence of CKD (55%,95% CI: 42.4-62.2) did not differ from those without diabetes (46%,95% CI: 41.9-50.0, p = 0.133). Conclusion: CKD is common in hypertensive patients in Riyadh, Saudi Arabia with a prevalence of 46.9%. This provided justification for the inclusion of this group in CKD screening programs in Saudi. [ABSTRACT FROM AUTHOR]

Background: Cancers and microbial infections are still a major health problem, therefore research on new anticancer and antimicrobial agents ought to be continued. Natural products including essential oils from medicinal plants continue to be an important resource to manage various diseases. Thus, the particular objectives of this study are to investigate the chemical composition, cytotoxic, antimicrobial and antioxidant activities of three Plectranthus species namely P. cylindraceus Hocst. ex Benth., P. asirensis JRI Wood and P. barbatus Andrews grown in Saudi Arabia. Methods: The essential oils of the three Plectranthus species were obtained by hydrodistllation and analyzed using GC/FID and GC-MS. The essential oils were further assessed for their cytotoxic, antimicrobial and antioxidant activities. Determination of the cytotoxic activity was carried out against Hela, HepG2 and HT-29 cancer cell lines by utilizing MTT-assay. The antimicrobial activity was assessed against six bacterial and fungal strains by using broth micro-dilution assay. In addition, the antioxidant activity was evaluated utilizing the DPPH and β-Carotene-linoleic acid assays. Results: The GC/FID and GC-MS analysis led to the identification of 59, 60 and 42 compounds representing 89.0% 95.0 and 97.1% of the total essential oils of P. cylindraceus, P. asirensis and P. barbatus, respectively. The essential oils were characterized by a high content of oxygenated sesquiterpenes in P. cylindraceus, sesquiterpene hydrocarbons in P. asirensis and monoterpene hydrocarbons in P. barbatus where maaliol (42.8%), β-caryophyllene (13.3%) and α-pinene, (46.2%) were the predominant compounds. Additionally, the oils particularly of P. cylindraceus and P. barbatus exhibited remarkable cytotoxic and antimicrobial activities with IC50-values between 3.8 and 7.5 μg/mL and MIC-values ranging from 0.137 to 4.40 mg/mL. Moreover, the oils showed moderate to high radical scavenging and antioxidative activities ranging from 52 to 75% at the highest concentration of 1 mg/mL. Conclusions: The observed results back the suggestion that these three Plectranthus species represent a promising source of cytotoxic and antimicrobial agents. [ABSTRACT FROM AUTHOR]

Liver disease is a worldwide problem. It represents one of the main causes of morbidity and mortality in humans. Achillea biebersteinii is used as herbal remedy for various ailments including liver diseases. But the scientific basis for its medicinal use remains unknown. Thus, this research was undertaken to evaluate the efficiency of A. biebersteinii essential oil (ABEO) (0.2 mL/kg) in the amelioration of CCl4-induced hepatotoxicity in rodent model. Moreover, the chemical content of the oil was investigated using GC and GC-MS. The following biochemical parameters were evaluated: serum glutamic oxaloacetic transaminase (GOT), glutamic-pyruvic transaminase (GPT), gamma-glutamyl-transpeptidase (γ-GGT), alkaline phosphatase (ALP), and total bilirubin. Furthermore, lipid profile, malondialdehyde (MDA), nonprotein sulfhydryl (NP-SH), and total protein (TP) contents in liver tissue were estimated. 44 components (92.0%) of the total oil have been identified by GC-MS analysis where α-terpinene and p-cymene were the most abundant. The high serum enzymatic (GOT, GPT, GGT, and ALP) and bilirubin concentrations as well as the level of MDA, NP-SH, and TP contents in liver tissues were significantly reinstated towards normalization by the ABEO. Histopathological study further confirmed these findings. In addition, ABEO showed mild antioxidant activity in 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and β-carotene-linoleic acid assays. [ABSTRACT FROM AUTHOR]

Metal levels in fish have been extensively studied, but little data currently exists for the Middle East. We examined the levels of metals and metalloids (aluminum, arsenic, copper, manganese, selenium, zinc, and mercury) in the flesh of 13 fish species collected from three fishing sites and a local fish market in Jeddah, Saudi Arabia. We tested the following null hypotheses: (1) there are no interspecific differences in metal levels, (2) there are no differences in metal levels in fishes between market and fishing sites, (3) there are no size-related differences in metal levels, and (4) there are no differences in selenium:mercury molar ratio among different fish species. There were significant interspecific differences in concentrations for all metals. There was an order of magnitude difference in the levels of aluminum, arsenic, mercury, manganese, and selenium, indicating wide variation in potential effects on the fish themselves and on their predators. Fishes from Area II, close to a large commercial port, had the highest levels of arsenic, mercury, and selenium, followed by market fishes. Mercury was positively correlated with body size in 6 of the 13 fish species examined. Mercury was correlated positively with arsenic and selenium, but negatively with aluminum, cobalt, copper, manganese, and zinc. Selenium:mercury molar ratios varied significantly among species, with Carangoides bajad, Cephalopholis argus, Variola louti, and Ephinephelus tauvina having ratios below 10:1. These findings can be used in risk assessments, design of mercury reduction plans, development of fish advisories to protect public health, and future management decision-making. [ABSTRACT FROM AUTHOR]

The antiplasmodial, antileishmanial, and antitrypanosomal activity of twenty-five medicinal plants distributed in Saudi Arabia and Yemen was evaluated. The plants were extracted withmethanol and screened in vitro against erythrocytic schizonts of Plasmodium falciparum, intracellular amastigotes of Leishmania infantum and Trypanosoma cruzi, and free trypomastigotes of T. brucei. To assess selectivity, cytotoxicity was determined on MRC-5 cells. Criteria for activity were an IC50 < 10 μg/mL and high selectivity (SI). Seven plants showed interesting antiprotozoal activity in one or more models. Extracts of Caralluma penicillata and Acalypha ciliata showed fairly good activity against P. falciparum with IC50 of 6.7 and 10.8 μg/mL and adequate selectivity (SI > 9.6 and > 5.9). Interesting activity against L. infantum was obtained with Verbascum bottae (IC50 of 3.2 μg/mL, SI 10.2) and Solanum glabratum (IC50 8.1 μg/mL, SI 3.4).The extracts of C. penicillata, Leucas virgata, Loranthus regularis, and V. bottae exhibited moderate activity against T. brucei (IC50 8.5, 8.1, 8.3, and 2.3 μg/mL; SI > 7.6, 7.7, 4.3, and > 14.1).These results partly support the traditional use of some of the selected medicinal plants and warrant further investigations into the putative active constituents. [ABSTRACT FROM AUTHOR]

Two lupane and four ursane triterpenes, namely epilupeol (1), lupeol acetate (2), ursolic acid (3), brein (4), 3β 11α-dihydroxy urs-12-ene (5) and ursolic acid lactone (6) were isolated from aerial parts of Kleinia odora and identified. Compounds 1 and 3-6 were isolated for the first time from K. odora. The triterpene constituents were investigated for antiprotozoal potential against erythrocytic schizonts of Plasmodium falciparum, intracellular amastigotes of Leishmania infantum and Trypanosoma cruzi and free trypomastigotes of T. brucei. Cytotoxicity was determined against MRC-5 fibroblasts to assess selectivity. The ursane triterpenes were found to be active against more than one type of the tested parasites, with the exception of compound 6. This is also the first report on the occurrence of ursane type triterpenes in the genus Kleinia and their antiprotozoal potential against P. falciparum, L. infantum, T. cruzi, and T. brucei. [ABSTRACT FROM AUTHOR]

The present study investigated the in vitro antiprotozoal activity of sixteen selected medicinal plants. Plant materials were extracted with methanol and screened in vitro against erythrocytic schizonts of Plasmodium falciparum, intracellular amastigotes of Leishmania infantum and Trypanosoma cruzi and free trypomastigotes of T. brucei. Cytotoxic activity was determined against MRC-5 cells to assess selectivity. The criterion for activity was an IC50 < 10 μg/mL (<5 μg/mL for T. brucei) and a selectivity index of ≥4. Antiplasmodial activity was found in the extracts of Prosopis juliflora and Punica granatum. Antileishmanial activity against L. infantum was demonstrated in Caralluma sinaica and Periploca aphylla. Amastigotes of T. cruzi were affected by the methanol extract of Albizia lebbeck pericarp, Caralluma sinaica, Periploca aphylla and Prosopius juliflora. Activity against T. brucei was obtained in Prosopis juliflora. Cytotoxicity (MRC-5 IC50 < 10 μg/mL) and hence non-specific activities were observed for Conocarpus lancifolius. [ABSTRACT FROM AUTHOR]

This study aimed at the identification of the spectrum of mutations in patients with ββ-thalassemia (ββ-thal) in the western province of Saudi Arabia. Screening for the mutations was done using the polymerase chain reaction-amplification refractory mutation system (PCR-ARMS) technique to test for 12 mutations, and direct automated DNA sequencing for the unknown samples. The study included 172 patients; of these 15 patients had sickle cell anemia and one Hb S [ββ6(A3)Glu→→Val, GAG>GTG]/ββ-thal. A total of 23 mutations were identified to cause the disease in the western area. Seven common mutations were responsible for the ββ-thal alleles in 78%% of patients and could be detected by the ARMS technique: IVS-II-1 (G>A), IVS-I-110 (G>A), IVS-I-5 (G>C), codon 39 (C>T), codon 26 (G>A) [Hb E or ββ26(B8)Glu→→Lys, GAG>AAG], frameshift codons (FSC) 8/9 (++G), and IVS-I-1 (G>A). DNA sequencing of uncharacterized alleles detected eight less common mutations: FSC 41/42 (--TCTT), IVS-I 25 bp deletion, codon 37 (G>A), FSC 44 (--C), Cap site ++1 (A>C), IVS-I-6 (T>C), FSC 5 (--CT) and IVS-I-1 (G>T), and eight rare mutations: −−87 (C>G), initiation codon −−1 (T>G), codon 15 (G>A), FSC 16 (--C), FSC 20/21 (++G), codon 27 (G>A), IVS-I-130 (G>C) and IVS-II-837 (A>C). Four alleles were normal by DNA sequencing. Genetic heterogeneity was observed in this study, 10 mutations were of Asian or Asian/Indian origin, two were Kurdish, one Chinese, one Turkish, one Saudi, and the remainder were of Mediterranean origin. The presence of a large population of immigrants in the western province is responsible for the great heterogeneity at the molecular level, and for the difference observed in the frequencies of mutations from those reported in the eastern province of Saudi Arabia. Screening for ββ-thal mutations using PCR-ARMS for the seven most frequent mutations in the Saudi population followed by DNA sequencing of the unknown alleles could be useful for the implementation of a strategy for carrier detection and preimplantation genetic diagnosis in high risk families. [ABSTRACT FROM AUTHOR]

Background and objective: The purpose of this study is to compare the attitudes, views, and factors that influence drug abuse among pharmacy and nursing students at a Saudi Arabian university. Materials and Methods: A cross-sectional study, was conducted among pharmacy and nursing students who are currently enrolled in the respective courses at the study site. The data were collected over 4 months from August to November 2019 using structured self-administered paper-based questionnaires. Results: Among the participants, pharmacy students accounted for 184 (58.2%) while 132 (41.8%) of the students were from nursing. More than a third of the students 129, (40.8%) smoked cigarettes. The majority of pharmacy (80.4%) and nursing students (67.4%) reported having undertaken a drug misuse course in college. Among the participants, 132 (41.7%) stated that an offer from friends, followed by joy seeking 129 (40.8%), parents' divorce 126 (39.8%), having access to drugs 125 (39.5%), family issues 110 (34.8%), 66 (20.8%) having a family member who is addicted, and 101 (31.9%) reported curiosity to be the factors regarding the use of abusive drugs. Transient euphoria (75.9%) followed by depression 197 (62.3%) was the most prevalent physical or psychological change that occurred following drug use. The family size and father's education have significantly affected the attitudes scores of the students (F = 5.188; p = 0.0001). Conclusion: In this study, joy-seeking, access to drugs, and family issues were found to be the major factors listed as reasons for drug abuse, with some of them being controllable or reversible. Educating about the adverse outcomes of abused drugs is warranted. [ABSTRACT FROM AUTHOR]

This article analyzes the 1990-1991 Gulf war foreign policy of Saudi Arabia. This policy during the Gulf war represents a watershed in the kingdom's conduct in international affairs. When Saudi Arabia sanctioned the use of U.S. force to crush Iraqi power just to end the war between Iraq and Iran, the kingdom has emphasised the primacy of the regional balance of power and its relations with the U.S. to preserve its security in the post-Gulf war era. Saudi Arabia's Gulf war policy also represents the culmination of unprecedented Saudi foreign policy activism. The 1973-74 Arab oil embargo and oil price increase thrust the kingdom into an Arab leadership role for which it was poorly prepared. In such political and economic circumstances, and as a result of them, Saudi foreign policy sought compromise and consensus.

The essential oil of Meriandra dianthera (Konig ex Roxb.) Benth. (Synonym: Meriandra bengalensis, Lamiaceae) collected from Saudi Arabia was studied utilizing GC and GC/MS. Forty four constituents were identified, representing 96.8% of the total oil. The M. dianthera essential oil (MDEO) was characterized by a high content of oxygenated monoterpenes (76.2%). Camphor (54.3%) was the major compound in MDEO followed by 1,8-cineole (12.2%) and camphene (10.4%). Moreover, MDEO was assessed for its cytotoxic, antimicrobial, and antioxidant activities. MDEO demonstrated an interesting cytotoxic activity against all cancer cell lines with IC50 values of 83.6 to 91.2 μg/mL, especially against MCF-7 cancer cells. Using labeling with annexin VFITC and/or propidium iodide (PI) dyes and flow cytometer analysis, the apoptosis induction was quantitatively confirmed for MCF-7 cells. The MDEO exhibited a considerable antimicrobial activity against all bacterial and fungal strains with minimum inhibitory concentration (MIC)-values of 0.07 to 1.25 mg/mL. The most sensitive microbial strain was Staphylococcus aureus (MIC: 0.07 mg/mL). Minimum bactericidal concentration (MBC) or minimum fungicidal concentration (MFC) values were determined one time higher than that of MIC's. Additionally, the MDEO revealed a strong activity for reducing β-carotene bleaching with a total antioxidant value of 72.6% and significant DPPH free radical scavenging activity (78.4%) at the concentration 1000 μg/mL. [ABSTRACT FROM AUTHOR]

In recent times, higher education institutions (HEIs) are seeking more attention in conducting surveys to improve the quality of teaching and achieve academic accreditation. In this regard, Imam Abdulrahman Bin Faisal University (IAU) also conducting various surveys to meet the requirements of the National Center for Academic Accreditation and evAluation (NCAAA), Saudi Arabia for attaining academic accreditation. Among those surveys, a survey named "Students Survey on Lecturing Skills (SSLS)" is conducted to reveal the students' perception of lecturing skills of instructors. In SSLS, the policymakers should reach the fixed new target benchmark for overall students' satisfaction during every academic year. Therefore, this study aimed to use stochastic modeling to predict the exact parameters which are to be focused by the instructors to improve their quality of teaching and enhance the overall students' satisfaction towards instructors' lecturing skills. The results showed that the instructors should focus on "Interest and motivation" (to enhance the overall students' satisfaction towards the lecturing skills by 99% in the presence of a 5% increase in target benchmark as per NCAAA standards during the upcoming academic year. This study indicates that the policymakers can make use of stochastic modeling in higher education to predict the exact parameters to be focused so that the cost and time spent would be reduced and make the process easier to achieve academic accreditation. [ABSTRACT FROM AUTHOR]

Background: For decades, practical clinical examinations have been valid and reliable methods to evaluate health sciences undergraduate students' clinical performance and competencies for a wide range of skills. Objective structured clinical examination (OSCE) is a widely used practical clinical examination. This study aims to assess health sciences students' perceptions about OSCE as an evaluation method of clinical skills acquisition. Methods: A descriptive cross-sectional design was used, and 157 students of five health science programs, who undergo real-time OSCE at Prince Sultan Military College of Health Sciences (PSMCHS), Dhahran, were invited to participate in this study. Data were collected through a structured questionnaire and analyzed quantitatively. Results: The findings revealed that 57.8% of the participants perceived OSCE positively due to its unique features related to structure, logical sequence, standardized score tools, and coverage of a broad spectrum of critical clinical skills. On the contrary, 62.3% (n=96) of participants believed OSCE was stressful, and 58.4% (n=90) thought it was threatening because of inadequate preparation. Conclusion: Health sciences students perceived OSCE as an effective evaluation method for clinical skills. However, OSCEs generate feelings of uncertainty and aggravate a high-stress level. This stress requires the educators at the academic and clinical settings to conduct dry run simulated scenarios beforehand to orient and guide students to manage and confound their stress and anxiety during real-life OSCE experiences. [ABSTRACT FROM AUTHOR]

In consideration of the emergence of novel drug-resistant microbial strains and the increase in the incidences of various cancers throughout the world, honey could be utilized as a great alternative source of potent bioactive compounds. In this context, this study pioneers in reporting the phytochemical profiling and the antimicrobial, antioxidant, and anticancer properties of Acacia honey (AH) from the Hail region of Saudi Arabia, assessed using in vitro and molecular docking approaches. The phytochemical profiling based on high-resolution liquid chromatography-mass spectrometry (HR-LCMS) revealed eight compounds and three small peptide-like proteins as the constituents. The honey samples exhibited promising antioxidant activities (DPPH-IC50 = 0.670 mg/mL; ABTS-IC50 = 1.056 mg/mL; β-carotene-IC50 > 5 mg/mL). In the well-diffusion assay, a high mean growth inhibition zone (mGIZ) was observed against Staphylococcus aureus (48.33 ± 1.53 mm), Escherichia coli ATCC 10536 (38.33 ± 1.53 mm), and Staphylococcus epidermidis ATCC 12228 (39.33 ± 1.15 mm). The microdilution assay revealed that low concentrations of AH could inhibit the growth of almost all the evaluated bacterial and fungal strains, with the minimal bactericidal concentration values (MBCs) ranging from 75 mg/mL to 300 mg/mL. On the contrary, high AH concentrations were required to kill the tested microorganisms, with the minimal bactericidal concentration values (MBCs) ranging from approximately 300 mg/mL to over 600 mg/mL and the minimal fungicidal concentration values (MFCs) of approximately 600 mg/mL. The AH exhibited effective anticancer activity in a dose-dependent manner against breast (MCF-7), colon (HCT-116), and lung (A549) cancer cell lines, with the corresponding IC50 values of 5.053 μg/mL, 5.382 μg/mL, and 6.728 μg/mL, respectively. The in silico investigation revealed that the observed antimicrobial, antioxidant, and anticancer activities of the constituent compounds of AH are thermodynamically feasible, particularly those of the tripeptides (Asp-Trp-His and Trp-Arg-Ala) and aminocyclitol glycoside. The overall results highlighted the potential of AH as a source of bioactive compounds with significant antimicrobial, antioxidant, and anticancer activities, which could imply further pharmacological applications of AH. [ABSTRACT FROM AUTHOR]

Fish are a healthy source of protein and nutrients, but contaminants in fish may provide health risks. Determining the risk from contaminants in fish requires site-specific information on consumption patterns. We examine consumption rates for resident and expatriates in the Jeddah region of Saudi Arabia, by species of fish and fishing location. For Saudis, 3.7% of males and 4.3% of females do not eat fish; for expatriates, the percent not eating fish is 6.6% and 6.1% respectively. Most people eat fish at home (over 90%), and many eat fish at restaurants (65% and 48%, respectively for Saudis and expatriates). Fish eaten at home comes from local fish markets, followed by supermarkets. Saudis included fish in their diets at an average of 1.4±1.2 meals/week at home and 0.8±0.7 meals/week at restaurants, while expats ate 2.0±1.7 meals/week at home and 1.1±1.1 meals/week in restaurants. Overall, Saudis ate 2.2 fish meals/week, while expats ate 3.1 meals/week. Grouper ( Epinephelus and Cephalopholis ) were eaten by 72% and 60% respectively. Plectropomus pessuliferus was the second favorite for both groups and Hipposcarus harid and Lethrinus lentjan were in 3rd and 4th place in terms of consumption. Average meal size was 68 g for Saudis and 128 g for expatriates. These data can be used by health professionals, risk assessors, and environmental regulators to examine potential risk from contaminants in fish, and to compare consumption rates with other sites. [ABSTRACT FROM AUTHOR]

The development of novel antimicrobial drugs, as well as the discovery of novel compounds able to promote honeybee's growth, represents major challenges for modern entomology. The main aim of this study was to investigate whether Brevibacillus laterosporus isolated from the digestive tract of Saudi honeybees, Apis mellifera, was able to stimulate colony strength parameters of honeybees and to evaluate its ability to produce antimicrobial agents. Honeybees were collected in Dirab, Riyadh Region, Saudi Arabia, and microorganisms were isolated and identified by 16S ribosomal RNA analysis. Microscopic identification of the microorganism in its native state was facilitated by atomic force microscopy at high-resolution imaging. Active biological compounds were produced by submerged fermentation with B. laterosporus. The fermented broth was subjected to extraction and purification, and then semi-pure compounds were analyzed by gas chromatography-mass spectrometry. The effectiveness of the crude extract and semi-pure compounds as antimicrobial agents was evaluated by susceptibility assays. More than 22% of the microorganisms isolated from the digestive tract of healthy honeybees have been identified as B. laterosporus, this kind of species has a unique shape and morphological structure. The cyclic dipeptide cyclo(Leu-Pro) produced by B. laterosporus showed biological activity against several pathogenic microorganisms. Furthermore, the total counts of workers, closed brood, and open brood, as well as the production of bee pollen and honey, were better in honeybees treated with a B. laterosporus suspension. The data indicated that the B. laterosporus strain isolated from a healthy honeybee might be a novel probiotic and a producer of important biological compounds.

Re-investigation of the EtOH extract of the aerial parts of Kleinia odora led to the isolation of two new triterpenes; klodorone A (3) and klodorol A (4), together with two known compounds: β-amyrin (1) and germanicol (2), which were reported from this plant for the first time. Their structures were determined by using extensive 1D ((1)H, (13)C and DEPT) and 2D ((1)H-(1)H COSY, HMQC and HMBC) NMR and mass spectral measurements in addition to comparison of their data with the literature.

Background: Worldwide particularly in developing countries, a large proportion of the population is at risk for tropical parasitic diseases. Several medicinal plants are still used traditionally against protozoal infections in Yemen and Saudi Arabia. Thus the present study investigated the in vitro antiprotozoal activity of twenty-five plants collected from the Arabian Peninsula., Methods: Plant materials were extracted with methanol and screened in vitro against erythrocytic schizonts of Plasmodium falciparum, intracellular amastigotes of Leishmania infantum and Trypanosoma cruzi and free trypomastigotes of T. brucei. Cytotoxic activity was determined against MRC-5 cells to assess selectivity. The criterion for activity was an IC(50) < 10 μg/ml (<5 μg/ml for T. brucei) and selectivity index of >4., Results: Antiplasmodial activity was found in the extracts of Chrozophora oblongifolia, Ficus ingens, Lavandula dentata and Plectranthus barbatus. Amastigotes of T. cruzi were affected by Grewia erythraea, L. dentata, Tagetes minuta and Vernonia leopoldii. Activity against T. brucei was obtained in G. erythraea, L. dentata, P. barbatus and T. minuta. No relevant activity was found against L. infantum. High levels of cytotoxicity (MRC-5 IC(50) < 10 μg/ml) and hence non-specific activities were noted in Cupressus sempervirens, Kanahia laniflora and Kniphofia sumarae., Conclusion: The results endorse that medicinal plants can be promising sources of natural products with antiprotozoal activity potential. The results support to some extent the traditional uses of some plants for the treatment of parasitic protozoal diseases.

Respiratory syncytial virus (RSV) has been recognized as a major nosocomial hazard on pediatric wards. Because of maternally acquired antibodies, symptomatic RSV infection is rare in term neonates. During an outbreak of RSV in our neonatal ICU, 12 infants (gestational age = 34 +/- 5 weeks) remained RSV negative. In contrast, eight preterm infants (gestational age = 28 +/- 2 weeks) became RSV positive. Four infants became very sick with RSV and required mechanical ventilation and support. Acute respiratory distress syndrome (ARDS) developed in two of them resulting in death of one of them. Control measures were effective in controlling the outbreak. We conclude that during an RSV outbreak in the neonatal ICU, the attack rate is higher in preterm infants born at lower gestational age resulting in significant mortality and morbidity.