1. Predicted bacteriorhodopsin from Exiguobacterium sibiricum is a functional proton pump.
- Author
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Petrovskaya LE, Lukashev EP, Chupin VV, Sychev SV, Lyukmanova EN, Kryukova EA, Ziganshin RH, Spirina EV, Rivkina EM, Khatypov RA, Erokhina LG, Gilichinsky DA, Shuvalov VA, and Kirpichnikov MP
- Subjects
- Amino Acid Sequence, Arctic Regions, Bacillales isolation & purification, Bacteriorhodopsins chemistry, Base Sequence, Cloning, Molecular, DNA Primers genetics, DNA, Bacterial genetics, Escherichia coli genetics, Escherichia coli metabolism, Genes, Bacterial, Molecular Sequence Data, Proton Pumps chemistry, Recombinant Proteins chemistry, Recombinant Proteins genetics, Recombinant Proteins metabolism, Russia, Spectrophotometry, Bacillales genetics, Bacillales metabolism, Bacteriorhodopsins genetics, Bacteriorhodopsins metabolism, Proton Pumps genetics, Proton Pumps metabolism
- Abstract
The predicted Exigobacterium sibiricum bacterirhodopsin gene was amplified from an ancient Siberian permafrost sample. The protein bacteriorhodopsin from Exiguobacterium sibiricum (ESR) encoded by this gene was expressed in Escherichia coli membrane. ESR bound all-trans-retinal and displayed an absorbance maximum at 534nm without dark adaptation. The ESR photocycle is characterized by fast formation of an M intermediate and the presence of a significant amount of an O intermediate. Proteoliposomes with ESR incorporated transport protons in an outward direction leading to medium acidification. Proton uptake at the cytoplasmic surface of these organelles precedes proton release and coincides with M decay/O rise of the ESR., (Copyright © 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.)
- Published
- 2010
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