1. Tepidibacillus marianensis sp. nov., a novel heterotrophic iron-reducing bacterium isolated from Mariana Trench sediment.
- Author
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Li S, Li C, Pei J, Liu R, Fang J, Wei Y, He Y, Li S, Feng Q, Zhang C, Guo T, Jiang Y, Hu Y, Jiang Z, Shi L, and Dong Y
- Subjects
- Russia, Heterotrophic Processes, Nucleic Acid Hybridization, Bacillaceae classification, Bacillaceae genetics, Bacillaceae isolation & purification, Whole Genome Sequencing, Oxidation-Reduction, Phylogeny, Base Composition, RNA, Ribosomal, 16S genetics, Fatty Acids, Geologic Sediments microbiology, DNA, Bacterial genetics, Bacterial Typing Techniques, Sequence Analysis, DNA, Iron metabolism, Phospholipids
- Abstract
A novel chemoheterotrophic iron-reducing micro-organism, designated as strain LSZ-M11000
T , was isolated from sediment of the Marianas Trench. Phylogenetic analysis based on the 16S rRNA gene revealed that strain LSZ-M11000T belonged to genus Tepidibacillus , with 97 % identity to that of Tepidibacillus fermentans STGHT , a mesophilic bacterium isolated from the Severo-Stavropolskoye underground gas storage facility in Russia. The polar lipid profile of strain LSZ-M11000T consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, as well as other unidentified phospholipids and lipids. The major fatty acids were C16 : 0 (28.4 %), C18 : 0 (15.8 %), iso-C15 : 0 (12.9 %), and anteiso-C15 : 0 (12.0 %). Strain LSZ-M11000T had no menaquinone. Genome sequencing revealed that the genome size of strain LSZ-M11000T was 2.97 Mb and the DNA G+C content was 37.9 mol%. The average nucleotide identity values between strain LSZ-M11000T and its close phylogenetic relatives, Tepidibacillus fermentans STGHT and Tepidibacillus decaturensis Z9T , were 76.4 and 72.6 %, respectively. The corresponding DNA-DNA hybridization estimates were 20.9 and 23.4 %, respectively. Cells of strain LSZ-M11000T were rod-shaped (1.0-1.5×0.3-0.5 µm). Using pyruvate as an electron donor, it was capable of reducing KMnO4 , MnO2 , As(V), NaNO3 , NaNO2 , Na2 SO4 , Na2 S2 O3 , and K2 Cr2 O7 . Based on phenotypic, genotypic, and phylogenetic evidence, strain LSZ-M11000T is proposed to be a novel strain of the genus Tepidibacillus , for which the name Tepdibacillus marianensis is proposed. The type strain is LSZ-M11000T (=CCAM 1008T =JCM 39431T ).- Published
- 2024
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