1. Anoxybacillus voinovskiensis sp. nov., a moderately thermophilic bacterium from a hot spring in Kamchatka.
- Author
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Yumoto I, Hirota K, Kawahara T, Nodasaka Y, Okuyama H, Matsuyama H, Yokota Y, Nakajima K, and Hoshino T
- Subjects
- Aerobiosis, Anaerobiosis, Bacillaceae cytology, Bacillaceae physiology, Bacterial Typing Techniques, Base Composition, Catalase metabolism, DNA, Bacterial chemistry, DNA, Ribosomal chemistry, Genes, rRNA, Gentian Violet, Growth Inhibitors pharmacology, Hydrogen Sulfide metabolism, Hydrogen-Ion Concentration, Molecular Sequence Data, Movement, Nitrates metabolism, Nucleic Acid Hybridization, Oxidation-Reduction, Oxidoreductases metabolism, Phenazines, Phylogeny, RNA, Bacterial genetics, RNA, Ribosomal, 16S genetics, Russia, Sequence Analysis, DNA, Sequence Homology, Sodium Chloride pharmacology, Temperature, Water Microbiology, Bacillaceae classification, Bacillaceae isolation & purification, Hot Springs microbiology
- Abstract
A novel moderately thermophilic bacterium, strain TH13T, was isolated from a hot spring in Kamchatka. It was found to be a Gram-positive, facultative aerobe; the straight, non-motile rods grew at 30-64 degrees C (optimum 54 degrees C). The isolate was positive for catalase and oxidase tests and reduced nitrate to nitrite, but was negative for H2S production and growth in more than 3% NaCl (w/v). The isolate grew at pH 7-8, but not at pH values higher than 9. The DNA G+C content was 43.9 mol%. Phylogenetic analysis based on 16S rRNA gene sequencing indicated that strain TH13T was a member of the genus Anoxybacillus. DNA-DNA hybridization revealed a low relatedness (less than 30.2%) between the isolate and its close phylogenetic neighbours Anoxybacillus pushchinoensis and Anoxybacillus flavithermus. On the basis of phenotypic characteristics, phylogenetic data and DNA-DNA hybridization data, it was concluded that the isolate merited classification as a novel species, for which the name Anoxybacillus voinovskiensis sp. nov. is proposed. The type strain of this species is TH13T (=NCIMB 13956T=JCM 12111T).
- Published
- 2004
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