1. Sapientia aquatica gen. nov., sp. nov., isolated from a crater lake.
- Author
-
Felföldi T, Szabó A, Tóth E, Schumann P, Kéki Z, Márialigeti K, and Máthé I
- Subjects
- Bacterial Typing Techniques, Base Composition, DNA, Bacterial genetics, Fatty Acids chemistry, Oxalobacteraceae isolation & purification, Phospholipids chemistry, Quinones chemistry, RNA, Ribosomal, 16S genetics, Romania, Sequence Analysis, DNA, Lakes microbiology, Oxalobacteraceae classification, Phylogeny, Water Microbiology
- Abstract
A new aerobic betaproteobacterium, strain SA-152
T , was isolated from the water of a crater lake. 16S rRNA gene sequence analysis revealed that strain SA-152T belonged to the family Oxalobacteraceae (order Burkholderiales ) and was phylogenetically related to Solimicrobium silvestre S20-91T with 97.09 % and to Herminiimonas arsenicoxydans ULPAs1T with 96.00 % 16S rRNA gene pairwise sequence similarity. Cells of strain SA-152T were rod-shaped, non-motile, oxidase-negative and catalase-positive. Its fatty acid profile was dominated by two fatty acids, C16 : 1 ω 7 c and C16 : 0 , the major respiratory quinones were Q-8 and Q-7, and the main polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The G+C content of the genomic DNA of strain SA-152T was 48.3 mol%. The new bacterium can be distinguished from closely related genera Solimicrobium , Herminiimonas , Rugamonas and Undibacterium based on its non-motile and oxidase-negative cells. On the basis of the phenotypic, chemotaxonomic and genomic data, strain SA-152T is considered to represent a novel species of a new genus, for which the name Sapientia aquatica gen. nov., sp. nov. is proposed. The type strain of Sapientia aquatica is SA-152T (=DSM 29805T =NCAIM B.02613T ).- Published
- 2020
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