1. COMPARATIVE MICROBIOLOGICAL CHARACTERIZATION OF THE URBAN SEWAGE SLUDGE AND RESIDUAL MARINE BIOMASS ALONG ROMANIAN BLACK SEA COAST.
- Author
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Ticuţa, Negreanu-Pirjol, Bogdan, Negreanu-Pirjol, Gabriela--Mihaela, Paraschiv, Rodica, Sirbu, and Aurelia, Meghea
- Subjects
PATHOGENIC microorganisms ,BIOMASS ,SEWAGE sludge ,BIOMOLECULES ,AGRICULTURE - Abstract
Pathogenic microorganisms can contaminate the environment. The role of these factors in producing transmissible diseases was proven by a number of authors long before the discovery of pathogenic microbial agents. Accepting the notion of bacterial contamination indicator of environmental, the necessity to establish when a nonpathogen or conditionally pathogen germ can be indicator appeared. If for pathogen germs their mere presence in the environment may be enough to consider the researched factor as dangerous, in reality the true is represented also by the number of present pathogens. For indicators germs establishing the number through quantitative and qualitative bacteriological examinations are necessary. In this aim, a comparative study of two residual biomass microbial charges (sewage sludge and marine biomass) was realized in order to assess the possibilities of combining them and identifying the optimal level of participation of each component for achieving a potential biocomposite fertilizer formula. The microbial loading problem has been the priority of our study because this is the most important factor involved in improvement of two biological wastes for agriculture purpose. Marine biomass (macroalgae and zoobenthos) along the seashore from Cap Singol of Constanta to the southern extremity of the Romanian Black Sea Coast consist of green, brown and red macroalgae, raw plant material Danube river origin, benthic marine organisms as crustacean decapods, bivalves and gastropods (mussels and Rapana sp.), shell material and the sewage sludge coming from urban wastewater treatment plants, were collected monthly in the last two years. Analysis methodology applied to residual marine biomass and sewage sludge has been based on aqueous extracts from dried samples. The follows physical-chemical parameters for the biomass extracts have been studied: moisture, ash, total nitrogen, total protein, total lipids and carbohydrates. For the microbial charge determination, the serial dilutions method has been applied and the qualitative and quantitative identification by the colony forming units reported to the volume unit (total coliforms, fecal coliforms, total streptococcus, fecal streptococcus, Shigella, Salmonella sp., Staphylococcus aureus, Campylobacter spp.), was realized. The comparative microbiological characterization assessed that the two residual biomasses could be successfully recovered as separately or mixed in the aim to obtain a new biocomposite fertilizer with decreased bacterial charge. [ABSTRACT FROM AUTHOR] more...
- Published
- 2012
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