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The correct technical and scientific understanding as well as the quality control protocols for the DNA analysis has been important in legal decisions in the light of technological development in the last 20 years. This study aimed to map the quality in DNA laboratories to meet the standards used by these laboratories. A survey was conducted to identify laboratories that work in forensics and quality requirements used. The results on the responses of 34.7% of laboratories ( N = 72) point distribution of 44% of laboratories in the axis Rio de Janeiro and São Paulo. The paternity testing and forensic analysis represent 72% of the purposes of the analysis of DNA in the country. For the assurance of the measurement results, the survey showed that 76% use internal control and reference materials. Regarding the participation in proficiency testing, relevant factor for the external control, 72% use such mechanism. As for the implementation of quality management systems, research shows that 20% have implanted system and 16% by implantation, with the standard ISO/IEC 17025 the reference to the forensic field. This survey concluded that DNA laboratories aimed at implementation of quality management systems to guarantee reliable and comparable measurement results nationally and internationally. In the search for these improvements and considering international guidelines, it is clear that the use of quality requirements are extremely important in order to harmonize procedures and allow to obtain reliable and comparable measurement results. [ABSTRACT FROM AUTHOR]

The geographical distribution of Campanulaceae species from Rio de Janeiro state is detailed based on field expeditions and analyses of herbarium specimens. Twenty‐seven species in six genera were recorded, 17 endemic to Brazil and two endemic to the state. The species were found mostly in High Altitude Grassland, and forest formations, mainly at altitudes above 1000 m asl. Some species were frequently found in anthropized areas, such as urban centers and roadsides. Only three species are currently listed as endangered, but more species may be threatened due to their restricted distribution and current loss of habitat. Some species are represented only by very old records. Due to the low floristic similarity and significant ecosystem diversity represented by the Conservation Units, each one is relevant and irreplaceable for Campanulaceae conservation in Rio de Janeiro state. [ABSTRACT FROM AUTHOR]

Mercury behavior upon resuspension of sediments from two impacted areas of Guanabara Bay was evaluated to assess worst-case methylmercury (MeHg) responses, under dark experimental conditions to prevent demethylation by photolysis. Study areas include the Rio de Janeiro Harbor (RJH) and the chlor-alkali plant–affected Meriti River (MR) estuary. Total mercury (THg) and MeHg concentrations were determined along 24-h experiments of sediment resuspension in the bay water in dark conditions. Fine-grained Meriti River (MR) estuary sediments had 8 times higher MeHg initial concentrations than sandy Rio de Janeiro Harbor (RJH) sediments (3.4 ± 0.29 vs. 0.41 ± 0.1 ng g−1, respectively). Though THg contents were uncorrelated with resuspension time, statistically significant correlations of MeHg (rs = 0.78) and %MeHg in relation to THg (rs = 0.86) with resuspension time were observed for RJH sediments, indicating net methylation only for this study site. These positive correlation trends correspond to a 2.8 times MeHg concentration increase (ΔMeHg = 0.75 ng g−1) and 4.4 times increase in %MeHg (Δ%MeHg = 1.0%), after 24 h of resuspension. This suggests that assessments of factors affecting the MeHg spatial–temporal variability and associated toxicity risks can be limited in some sites if concentration changes due to sediment resuspension-redeposition processes are not considered. Therefore, the inclusion of MeHg evaluation before and after sediment resuspension events is recommendable for the improvement of dredging licensing and monitoring activities. [ABSTRACT FROM AUTHOR]

Copyright of Sustainability in Debate / Sustentabilidade em Debate is the property of University of Brasilia, Center for Sustainable Development and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Trabalhos em Lingüística Aplicada is the property of Universidade Estadual de Campinas - Portal de Periodicos Eletronicos Cientificos and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Dengue virus (DENV) represents a major threat to public health worldwide. Early DENV diagnosis should not only detect the infection but also identify patients with a higher likelihood to develop severe cases. Previous studies have suggested the potential for NS1 to serve as a viral marker for dengue severity. However, further studies using different sera panels are required to confirm this hypothesis. In this context, we developed a lab-based ELISA to detect and quantitate NS1 protein from the four DENV serotypes and from primary and secondary cases. This approach was used to calculate the circulating NS1 concentration in positive samples. We also tested the NS1 positivity of DENV-positive samples according to the Platelia Dengue NS1 Ag assay. A total of 128 samples were positive for DENV infection and were classified according to the WHO guidelines. The overall NS1 positivity was 68% according to the Platelia assay, whereas all samples were NS1-positive when analyzed with our lab-based ELISA. Fifty-four samples were positive by PCR, revealing a co-circulation of DENV1 and DENV4, and the NS1 positivity for DENV4 samples was lower than that for DENV1. The circulating NS1 concentration ranged from 7 to 284 ng/mL. Our results support previous data indicating the low efficiency of the Platelia assay to detect DENV4 infection. Moreover, this work is the first to analyze NS1 antigenemia using retrospective samples from a Brazilian outbreak. [ABSTRACT FROM AUTHOR]

Copyright of Acta Scientiarum: Agronomy is the property of Universidade Estadual de Maringa and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)