1. High prevalence of extended-spectrum beta-lactamases and plasmid-mediated AmpC beta-lactamases in Enterobacteriaceae isolated from long-term care facilities in Korea.
- Author
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Yoo JS, Byeon J, Yang J, Yoo JI, Chung GT, and Lee YS
- Subjects
- Anti-Bacterial Agents pharmacology, Bacterial Proteins classification, Bacterial Proteins genetics, Cross Infection microbiology, DNA Primers genetics, DNA, Bacterial genetics, Escherichia coli drug effects, Escherichia coli isolation & purification, Humans, Klebsiella pneumoniae drug effects, Klebsiella pneumoniae isolation & purification, Long-Term Care, Microbial Sensitivity Tests, Polymerase Chain Reaction methods, Prevalence, Republic of Korea, beta-Lactamases classification, beta-Lactamases genetics, Bacterial Proteins biosynthesis, Escherichia coli enzymology, Escherichia coli Infections microbiology, Klebsiella Infections microbiology, Klebsiella pneumoniae enzymology, beta-Lactamases biosynthesis
- Abstract
The aim of this study was to investigate for the first time the prevalence and types of extended-spectrum beta-lactamase (ESBL) and plasmid-mediated AmpC beta-lactamase (PABL) in Enterobacteriaceae in Korean long-term care facilities (LTCFs). ESBL rates were 45.3% (72/159) in Escherichia coli and 42.7% (50/117) in Klebsiella pneumoniae. In E. coli, CTX-M-28, the most prevalent ESBLs, was identified for the first time in Korea in 44 isolates. In K. pneumoniae, SHV-12 was found in 27 isolates (52.9% of ESBLs), among which 25 isolates also contained SHV-11. Twenty-seven isolates had SHV and CTX-M beta-lactamase simultaneously. PABL genes were detected in 39.3% (46/117) of K. pneumoniae and 3.1% (5/159) E. coli. In E. coli, DHA-1(3), CMY-2(1), and CMY-6(1) were detected, whereas in K. pneumoniae, only DHA-1 was detected. Among the PABL-producing organisms, 80.0% (E. coli) and 52.2% (K. pneumoniae) simultaneously produced ESBLs. In conclusion, LTCF residents in Korea have a very high prevalence of E. coli and K. pneumoniae producing ESBLs, PABLs, or both, and the genotypes of ESBL and PABL were identical with those found in general hospitals.
- Published
- 2010
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