1. Naasia aerilata gen. nov., sp. nov., a member of the family Microbacteriaceae isolated from air.
- Author
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Weon HY, Kim SJ, Jang YH, Hamada M, Tamura T, Ahn JH, Suzuki KI, and Kwon SW
- Subjects
- Actinomycetales genetics, Actinomycetales isolation & purification, Bacterial Typing Techniques, DNA, Bacterial genetics, Fatty Acids analysis, Molecular Sequence Data, Peptidoglycan analysis, Phospholipids analysis, RNA, Ribosomal, 16S genetics, Republic of Korea, Sequence Analysis, DNA, Vitamin K 2 analysis, Actinomycetales classification, Air Microbiology, Phylogeny
- Abstract
A Gram-positive, aerobic, rod-shaped bacterial strain, 5116S-4(T), was isolated from an air sample collected in Suwon city, Republic of Korea. Phylogenetic analysis based on 16S rRNA gene sequences indicated that this strain was a new member of the family Microbacteriaceae. The sequence similarities of this strain to the members of the family Microbacteriaceae were less than 97 %, with the highest values observed with Cryobacterium mesophilum MSL-15(T) (96.3 %), Cryobacterium roopkundense RuGl7(T) (96.2 %), Labedella gwakjiensis KSW2-17(T) (96.2 %), Cryobacterium luteum Hh15(T) (96.2 %), Cryobacterium psychrophilum DSM 4854(T) (96.2 %), Klugiella xanthotipulae 44C3(T) (96.0 %) and Amnibacterium kyonggiense KSL51201-037(T) (96.0 %). According to the phylogenetic tree, strain 5116S-4(T) formed a cluster with A. kyonggiense KSL51201-037(T), Labedella gwakjiensis KSW2-17(T) and Lysinimonas soli SGM3-12(T) (95.3 % sequence similarity) on the support of high bootstrap values. Cells were motile with single polar flagellum and showed optimum growth at 30 °C and pH 7 without NaCl. Predominant cellular fatty acids were anteiso-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0. Polar lipids were diphosphatidylglycerol, dimannosyldiacylglycerol, phosphatidylglycerol, one unknown glycolipid, one unknown phospholipid and one unknown lipid. The acyl type of the cell-wall muramic acid was the acetyl type. Peptidoglycan was supposed to be the type B1 with 2,4-diaminobutyric acid on position 3. Strain 5116S-4(T) was clearly distinguishable from the phylogenetically related genera in the family Microbacteriaceae in terms of chemotaxonomic characteristics. On the basis of the chemotaxonomic and phylogenetic characteristics, a novel genus and species are proposed, Naasia aerilata gen. nov., sp. nov. The type strain of Naasia aerilata is 5116S-4(T) ( = KACC 15517(T) = NBRC 108725(T)).
- Published
- 2013
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