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A Gram-positive, aerobic, rod-shaped bacterial strain, 5116S-4(T), was isolated from an air sample collected in Suwon city, Republic of Korea. Phylogenetic analysis based on 16S rRNA gene sequences indicated that this strain was a new member of the family Microbacteriaceae. The sequence similarities of this strain to the members of the family Microbacteriaceae were less than 97 %, with the highest values observed with Cryobacterium mesophilum MSL-15(T) (96.3 %), Cryobacterium roopkundense RuGl7(T) (96.2 %), Labedella gwakjiensis KSW2-17(T) (96.2 %), Cryobacterium luteum Hh15(T) (96.2 %), Cryobacterium psychrophilum DSM 4854(T) (96.2 %), Klugiella xanthotipulae 44C3(T) (96.0 %) and Amnibacterium kyonggiense KSL51201-037(T) (96.0 %). According to the phylogenetic tree, strain 5116S-4(T) formed a cluster with A. kyonggiense KSL51201-037(T), Labedella gwakjiensis KSW2-17(T) and Lysinimonas soli SGM3-12(T) (95.3 % sequence similarity) on the support of high bootstrap values. Cells were motile with single polar flagellum and showed optimum growth at 30 °C and pH 7 without NaCl. Predominant cellular fatty acids were anteiso-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0. Polar lipids were diphosphatidylglycerol, dimannosyldiacylglycerol, phosphatidylglycerol, one unknown glycolipid, one unknown phospholipid and one unknown lipid. The acyl type of the cell-wall muramic acid was the acetyl type. Peptidoglycan was supposed to be the type B1 with 2,4-diaminobutyric acid on position 3. Strain 5116S-4(T) was clearly distinguishable from the phylogenetically related genera in the family Microbacteriaceae in terms of chemotaxonomic characteristics. On the basis of the chemotaxonomic and phylogenetic characteristics, a novel genus and species are proposed, Naasia aerilata gen. nov., sp. nov. The type strain of Naasia aerilata is 5116S-4(T) ( = KACC 15517(T) = NBRC 108725(T)).

A Gram-stain-positive, non-motile rod, designated strain SGM3-12(T), was isolated from paddy soil in Suwon, Republic of Korea. 16S rRNA gene sequence analysis revealed that the strain represented a novel member of the family Microbacteriaceae. The nearest phylogenetic neighbour was Leifsonia kribbensis MSL-13(T) (97.4 % 16S rRNA gene sequence similarity). Strain SGM3-12(T) and Leifsonia kribbensis MSL-13(T) formed a distinct cluster within the family Microbacteriaceae. Strain SGM3-12(T) contained MK-12(H2) and MK-11(H2) as the predominant menaquinones with moderate amounts of MK-12 and MK-11; anteiso-C15 : 0 and iso-C16 : 0 as the major cellular fatty acids (>10 % of total); and diphosphatidylglycerol, phosphatidylglycerol and unidentified glycolipids as the polar lipids. The peptidoglycan type of the isolate was B1δ with L-Lys as the diagnostic cell-wall diamino acid. On the basis of these results, strain SGM3-12(T) represents a novel species within a new genus, for which the name Lysinimonas soli gen. nov., sp. nov. is proposed (the type strain of the type species is SGM3-12(T) = KACC 13362(T) = NBRC 107106(T)). It is also proposed that Leifsonia kribbensis be transferred to this genus as Lysinimonas kribbensis comb. nov. (the type strain is MSL-13(T) = DSM 19272(T) = JCM 16015(T) = KACC 21108(T) = KCTC 19267(T)).

A novel actinomycete strain, 7402J-48(T), was isolated from an air sample collected from Jeju island, Republic of Korea. Cells were gram-positive, aerobic, flagellated, short rods. Strain 7402J-48(T) showed highest 16S rRNA gene sequence similarity (98.6 %) with Angustibacter luteus TT07R-79(T), and had relatively low sequence similarities (below 95.1 %) with other members of the family Kineosporiaceae. The cell wall of strain 7402J-48(T) contained alanine, glutamic acid and 2,6-diaminopimelic acid, suggesting A1γ-type peptidoglycan. The menaquinones were MK-9(H(4)) and MK-8(H(4)). The acyl type of the cell-wall muramic acid was acetyl. Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside and an unknown lipid were present. The cellular fatty acid profile comprised a large amount of anteiso-C(15 : 0), moderate amounts of C(16 : 0), summed feature 3 (including C(16 : 1)ω7c and/or C(16 : 1)ω6c), C(17 : 0), iso-C(15 : 0), C(16 : 0) N alcohol and C(17 : 1)ω8c, and small amounts of other fatty acids. The DNA G+C content of strain 7402J-48(T) was 73 mol%. Based on its phenotypic and genotypic characteristics, strain 7402J-48(T) ( = KACC 15527(T) = NBRC 108730(T)) is proposed as the type strain of a novel species, Angustibacter aerolatus sp. nov. An emended description of the genus Angustibacter is provided.

A bacterial strain isolated from an air sample, strain 5317J-19(T), was characterized. The isolate was an aerobic, motile, Gram-positive rod. The organism was able to grow between 4 and 35°C and between pH 6 and 9. The predominant fatty acids were anteiso-C(15:0) and iso-C(16:0). The major respiratory menaquinones were MK-12 and MK-11, and the minor ones were MK13, MK-10, and MK-9. Genomic DNA G+C content was 66 mol%. The diagnostic diamino acid of the peptidoglycan is presumably D-Orn. The peptidoglycan is supposed to be B2β type. The 16S rRNA gene sequence analysis indicated that this isolate belongs to the family Microbacteriaceae and had the highest sequence similarities with Salinibacterium xinjiangense 0543(T) (97.6%), Salinibacterium amurskyense KMM 3673(T) (97.2%), and Leifsonia bigeumensis MSL-27(T) (97.2%). Phylogenetic analysis and phenotypic characteristics support the proposal of a new genus and a novel species, with the name Homoserinimonas aerilata gen. nov., sp. nov. The type strain of Homoserinimonas aerilata is 5317J-19(T) (=KACC 15522(T) =NBRC 108729(T)).

The bacterial strain M1T8B10(T) was isolated from cow dung in Suwon, Republic of Korea. The strain was a Gram stain-positive rod, nonmotile, and non-spore-forming. According to 16S rRNA gene sequence analysis, the strain fell within the clade of the genus Leucobacter, showing the highest sequence similarities with Leucobacter aridicollis L-9(T) (98.7%), Leucobacter iarius 40(T) (98.4%), and Leucobacter komagatae JCM 9414(T) (98.2%). Cell-wall peptidoglycan contained the diagnostic diamino acid 2,4-diaminobutyric acid of the genus Leucobacter, showing B-type cross-linked peptidoglycans. The major fatty acids were anteiso-C(15:0), iso-C(16:0), and anteiso-C(17:0). The quinone system consisted of the menaquinones MK-11 (78%) and MK-10 (22%). The polar lipid profiles contained diphosphatidylglycerol, phosphatidylglycerol, and an unidentified glycolipid. Differences in several physiological features including nitrate reduction enabled the isolate to be differentiated from all recognized Leucobacter species. Based on these phylogenetic, chemotaxonomic, and phenotypic results, the isolate represents a novel species, for which the name Leucobacter denitrificans sp. nov. is proposed. The type strain is M1T8B10(T) (=KACC 14055(T) =NBRC 106309(T)).

An actinomycete strain, designated M1T8B9(T), was isolated from cow dung in Suwon, Republic of Korea. The isolate was a Gram-positive, nonmotile, and non-spore-forming bacterium. Phylogenetic evaluation based on 16S rRNA gene sequence similarity showed that this isolate belongs to the genus Microbacterium, with its closest neighbors being Microbacterium soli DCY17(T) (98.2%) and Microbacterium esteraromaticum DSM 8609(T) (98.0%). The polar lipid pattern consisted of diphosphatidylglycerol, phosphatidylglycerol, and one unknown glycolipid. Strain M1T8B9(T) contained the major fatty acids C(15:0) anteiso, C(16:0) iso, C (17:0) anteiso, and C(15:0) iso, and the cell-wall peptidoglycan was of type B2β. According to DNA-DNA hybridization studies, strain M1T8B9(T) showed 42% and 26% relatedness with M. soli DCY17(T) and M. esteraromaticum DSM 8609(T), respectively. On the basis of the data presented, strain M1T8B9(T) is considered to represent a novel species of the genus Microbacterium, for which the name Microbacterium suwonense sp. nov. is proposed. The type strain is M1T8B9(T) (=KACC 14058(T) =NBRC 106310(T)).