1. Sesquiterpenoids from the Aerial Parts of Salvia plebeia with Inhibitory Activities on Proprotein Convertase Subtilisin/Kexin Type 9 Expression.
- Author
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Nhoek P, Chae HS, Kim YM, Pel P, Huh J, Kim HW, Choi YH, Lee K, and Chin YW
- Subjects
- Hep G2 Cells, Humans, Molecular Structure, Phytochemicals isolation & purification, Phytochemicals pharmacology, Plant Components, Aerial chemistry, Proprotein Convertase 9 metabolism, Receptors, LDL metabolism, Republic of Korea, Sesquiterpenes isolation & purification, PCSK9 Inhibitors, Salvia chemistry, Sesquiterpenes pharmacology
- Abstract
Phytochemical investigation of the methanol extract of the aerial parts of Salvia plebeia aided by a proprotein convertase subtilisin/kexin type 9 (PCSK9) mRNA expression screening assay in HepG2 cells led to the identification of 19 compounds including one new norsesquiterpene ( 1 ), six new eudesmane sesquiterpenoids ( 2 - 5 , 8 , and 11 ), and 12 known compounds. The structures of all compounds were elucidated by interpretation of their 1D and 2D NMR spectroscopic and MS data. Furthermore, computational prediction of ECD or chemical shifts was used to propose the absolute configurations of the new structures. All isolates were assessed for their inhibitory activities against PCSK9 mRNA expression and PCSK9-low-density lipoprotein receptor (LDLR) interactions. None of the isolated compounds inhibited PCSK9 and LDLR interactions. However, compounds 1 , 9 , and 10 downregulated PCSK9 mRNA expression.
- Published
- 2021
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