12 results on '"CHYTRIDIOMYCOSIS"'
Search Results
2. Characterisation of microsatellites for Litoria nannotis (Amphibia : Hylidae), an endangered waterfall frog endemic to the Australian Wet Tropics.
- Author
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Puschendorf, Robert, Todd, Erica V., and Gardner, Michael G.
- Subjects
- *
HYLIDAE , *MICROSATELLITE repeats , *ENDANGERED species , *CHYTRIDIOMYCOSIS , *WATERFALLS - Abstract
Litoria nannotis is an endangered waterfall frog from the wet tropics region in north Queensland that has suffered significant population declines due to the emerging fungal disease known as chytridiomycosis. The species has two deeply divergent lineages, and we used 454 shotgun sequencing of DNA extracted from one individual of the northern lineage to identify and design PCR primers for 576 microsatellite loci. Thirty markers were tested for amplification success and variability in a population sample from each lineage. Of these, 17 were found to be polymorphic in the northern lineage and 10 loci were polymorphic in the southern lineage. Numbers of alleles per locus ranged from 2 to 14 (mean = 6.47, s.d. = 4.02) for the northern lineage (17 polymorphic loci), and from 2 to 8 (mean = 5.40, s.d. = 2.55) in the southern lineage (10 polymorphic loci). Levels of heterozygosity were high in both lineages (northern meanHE = 0.63, s.d. = 0.21, range = 0.27-0.89; southern mean HE = 0.57, s.d. = 0.25, range = 0.18-0.81). These loci will be useful in understanding the genetic variation and connectivity amongst populations of this species recovering from mass population declines due to disease. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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3. Urinary corticosterone metabolites and chytridiomycosis disease prevalence in a free-living population of male Stony Creek frogs (Litoria wilcoxii)
- Author
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Kindermann, Christina, Narayan, Edward J., and Hero, Jean-Marc
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- *
CORTICOSTERONE , *METABOLITES , *CHYTRIDIOMYCOSIS , *FROGS , *AMPHIBIAN diseases , *ZOOSPORES - Abstract
Abstract: The emerging amphibian disease chytridiomycosis, which is caused by the fungal pathogen (Batrachochytrium dendrobatidis, Bd), has caused mass mortalities of native amphibian populations globally. There have been no previous studies on the relationships between stress hormones in free-living amphibians and Bd infections. In this study, we measured urinary corticosterone metabolite concentrations and Bd infections within free-living populations of male Stony Creek frog (Litoria wilcoxii) in Queensland, Australia. Prevalence of Bd zoospores from frog skin swabs was quantified using a real-time quantitative PCR technique. A urinary corticosterone enzyme-immunoassay (EIA) was validated using adrenocorticotropic hormone (ACTH) challenge. Urinary corticosterone concentrations of male frogs increased within 1–2days after ACTH challenge and returned to baseline levels within 3days post-ACTH injection. None of the frogs showed any rise in urinary corticosterone after saline injections. Individual male frogs showed either low or high baseline corticosterone concentrations. Male frogs identified as positive for Bd infection had significantly higher baseline urinary corticosterone concentrations in comparison to Bd negative male frogs. Urinary corticosterone EIA provides a reliable indication of stress in this frog species and this non-invasive physiological tool can be used to further assess the dynamics of Bd infections and physiological stress responses in other native amphibians. [Copyright &y& Elsevier]
- Published
- 2012
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4. Validation of Diagnostic Tests in Wildlife: The Case of Chytridiomycosis in Wild Amphibians.
- Author
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Skerratt, Lee F., Mendez, Diana, McDonald, Keith R., Garland, Stephen, Livingstone, James, Berger, Lee, and Speare, Richard
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CHYTRIDIOMYCOSIS , *NONINVASIVE diagnostic tests , *AMPHIBIANS , *HISTOLOGY , *DIAGNOSIS - Abstract
We validated the diagnostic tests for the high-profile disease, chytridiomycosis, in wild amphibian populations. We compared histological samples with a Taqman real time quantitative PCR (qPCR) test in five species of frogs at different times of the year at six locations in the wet tropics of northern Queensland. The sensitivity and specificity of each test were estimated using prior estimates from previous laboratory studies and Bayesian methods. The qPCR test was almost three times as sensitive 72.9% (62.7-82.2%) than histology 26.5% (19.9-33.9%) but was less specific 94.2% (89.3-98.6%) than histology 99.5% (98.4-100%), which was likely caused by contamination. Monitoring of the negative control success rate of the qPCR test is potentially a good indicator of specificity. It is likely that using individual gloves for handling amphibians reduces cross-contamination and, therefore, improves specificity rather than cause inhibition of the qPCR. Classifying indeterminate results as positive will increase the qPCR test sensitivity but will lower specificity to a lesser degree depending on the likelihood of contamination. Although PCR is the preferred test for amphibian populations, histology is useful when wishing to confirm a diagnosis of infection and in situations where observing the severity of infection and pathology in skin is desired. In this study, we show that diagnostic test validation in wild animals is now relatively straight forward using modern computing power and can incorporate prior knowledge generated from laboratory studies using Bayesian approaches. [ABSTRACT FROM AUTHOR]
- Published
- 2011
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5. Application of the survey protocol for chytridiomycosis to Queensland, Australia.
- Author
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Skerratt, Lee F., McDonald, Keith R., Hines, Harry B., Berger, Lee, Mendez, Diana, Phillott, Andrea D., Cashins, Scott D., Murray, Kris A., and Speare, Richard
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BATRACHOCHYTRIUM dendrobatidis ,CHYTRIDIOMYCOSIS ,MYCOSES ,SURVEYS ,BIOGEOGRAPHY - Abstract
The article discusses a study which applied the survey protocol for amphibian chytrid fungus Batrachochytrium dendrobatidis (Bd) by attempting to systematically map its distribution in Queensland. With the use of the protocol, the study easily detected Bd in known infected areas such as the Wet Tropics and South East Queensland. Regions where Bd was not detected have reportedly bordered infected regions for between 15 and 30 years. Data from the study also supports biogeographic climatic models that show much of inland and northern Australia to be too hot and dry to support the fungus.
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- 2010
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6. Altitudinal distribution of chytrid ( Batrachochytrium dendrobatidis) infection in subtropical Australian frogs.
- Author
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KRIGER, KERRY M. and HERO, JEAN-MARC
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BATRACHOCHYTRIUM dendrobatidis , *CHYTRIDIALES , *FROGS , *INFECTION , *FUNGI - Abstract
The disappearance of amphibian populations from seemingly pristine upland areas worldwide has become a major focus of conservation efforts in the last two decades, and a parasitic chytrid fungus, Batrachochytrium dendrobatidis, is thought to be the causative agent of the population declines. We examined the altitudinal distribution of chytrid infections in three stream-dwelling frog species ( Litoria wilcoxii, L. pearsoniana and L. chloris) in southeast Queensland, Australia, and hypothesized that if B. dendrobatidis were responsible for the disappearance of high-altitude frog populations, infection prevalence and intensity would be greatest at higher altitudes. Overall, 37.7% of the 798 adult frogs we sampled were infected with B. dendrobatidis, and infections were found in all the populations we examined. Contrary to our initial hypothesis, we found no consistent evidence that high-altitude frogs were more likely to be infected than were lowland frogs. Further, the intensity of fungal infections (number of zoospores) on high-altitude frogs did not differ significantly from that of lowland frogs. Batrachochytrium dendrobatidis appears to be capable of infecting frogs at all altitudes in the subtropics, suggesting that all populations are at risk of decline when conditions favour disease outbreaks. We did find evidence, however, that chytrid infections persist longer into summer in upland as compared with lowland areas, suggesting that montane amphibian populations remain susceptible to disease outbreaks for longer periods than do lowland populations. Further, we found that at high altitudes, temperatures optimal for chytrid growth and reproduction coincide with frog metamorphosis, the life-stage at which frogs are most susceptible to chytrid infections. While these altitudinal differences may account for the differential population-level responses to the presence of B. dendrobatidis, the reason why many of southeast Queensland's montane frog populations declined precipitously while lowland populations remained stable has yet to be resolved. [ABSTRACT FROM AUTHOR]
- Published
- 2008
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7. The chytrid fungus Batrachochytrium dendrobatidis is non-randomly distributed across amphibian breeding habitats.
- Author
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Kriger, Kerry M. and Hero, Jean-Marc
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BATRACHOCHYTRIUM dendrobatidis , *CHYTRIDIALES , *AMPHIBIAN populations , *FROG populations , *WILDLIFE conservationists , *HEALTH services administration , *BODIES of water , *ANIMAL specialists - Abstract
The chytrid fungus Batrachochytrium dendrobatidis has been implicated as the causative agent of mass mortalities, population declines, and the extinctions of stream-breeding amphibian species worldwide. While the factors that limit the distribution and abundance of B. dendrobatidis across large geographical regions are fairly well understood, little is known about the distribution of the fungus within localized areas such as individual catchments. The accurate identification of amphibian populations likely to be exposed to the fungus is urgently required for effective disease management. We conducted disease surveys of frogs representing five ecological guilds in south-east Queensland, Australia, and hypothesized that if B. dendrobatidis were responsible for the disappearance of stream-breeding amphibian populations, infection prevalence and intensity would be greatest in frogs breeding in permanent, flowing water. Overall, 30.3% of the 519 frogs we sampled were infected with B. dendrobatidis. However, infections were not evenly distributed across the ecological guilds, being almost completely restricted to frogs breeding at permanent waterbodies. Of these, stream breeders were significantly more likely to be infected than were pond breeders, though the intensity of frogs’ infections did not differ significantly between the two guilds. Batrachochytrium dendrobatidis was detected on only one of the 117 frogs that were found at ephemeral ponds, ephemeral streams, or terrestrial sites. These findings provide strong support for the hypothesis that B. dendrobatidis was responsible for many of the unexplained disappearances of stream-breeding amphibian populations in recent decades, and will enable wildlife managers to more accurately focus conservation efforts on those species at highest risk of disease-related decline. [ABSTRACT FROM AUTHOR]
- Published
- 2007
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8. The Amphibian Chytrid Batrachochytrium dendrobatidis Occurs on Freshwater Shrimp in Rain Forest Streams in Northern Queensland, Australia.
- Author
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Rowley, Jodi, Alford, Ross, and Skerratt, Lee
- Subjects
SHRIMPS ,AMPHIBIAN populations ,AMPHIBIAN declines ,RAIN forests - Abstract
Chytridiomycosis is a disease of amphibians caused by the chytrid fungus Batrachochytrium dendrobatidis. It can be highly virulent and is unusual in that it appears to drive many host species to local extinction during outbreaks. One mechanism that could facilitate this is the ability to grow saprophytically or on alternative hosts. This is common in other chytrids but has not been demonstrated for B. dendrobatidis in the field. B. dendrobatidis can grow on arthropod exoskeletons in the laboratory, and freshwater shrimp can be the most abundant animals in tropical rain forest streams. We therefore used diagnostic quantitative polymerase chain reaction to determine the infection status of freshwater shrimp from areas in which they are sympatric with frog species that have suffered declines in association with outbreaks of chytridiomycosis. We detected B. dendrobatidis on three individual shrimp belonging to two genera and collected from two widely separated streams . Two of the individuals had high levels of infection. This indicates that the presence of alternative hosts is likely to contribute to the extreme virulence of chytridiomycosis outbreaks in some systems. The presence of alternative hosts may allow B. dendrobatidis to remain in the environment after local extinctions of amphibian hosts, preventing the recovery of amphibian populations. [ABSTRACT FROM AUTHOR]
- Published
- 2006
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9. Disease Exposure and Antifungal Bacteria on Skin of Invasive Cane Toads, Australia.
- Author
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Weitzman, Chava L., Kaestli, Mirjam, Gibb, Karen, Brown, Gregory P., Shine, Richard, and Christian, Keith
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RHINELLA marina , *INTRODUCED species , *BATRACHOCHYTRIUM dendrobatidis , *SKIN , *MICROBIAL communities , *SKIN microbiology , *ANIMAL experimentation , *ANTIFUNGAL agents , *ANURA , *BACTERIA , *COMPARATIVE studies , *FUNGI , *RESEARCH methodology , *MEDICAL cooperation , *RESEARCH , *EVALUATION research , *PHARMACODYNAMICS - Abstract
Cane toads, an invasive species in Australia, are resistant to fungal pathogens affecting frogs worldwide (Batrachochytrium dendrobatidis). From toad skin swabs, we detected higher proportions of bacteria with antifungal properties in Queensland, where toad and pathogen distributions overlap, than in other sites. This finding suggests that site-specific pathogen pressures help shape skin microbial communities. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
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10. 'Gatorade for frogs' delays fungal killer.
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CHYTRIDIOMYCOSIS , *ELECTROLYTE solutions , *FROG diseases - Abstract
The article reports on a study which found that the death of frogs with chytridiomycosis may be delayed by giving them an oral electrolyte-replacement solution. Caused by the lethal chytrid fungus, the disease impairs the skin's ability to take up sodium and potassium ions from water, according to Jamie Voyles of James Cook University in Townsville, Queensland and his colleagues. Voyles adds that their discovery may lead to the development of an effective treatment for the disease.
- Published
- 2009
- Full Text
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11. The return of the frogs: The importance of habitat refugia in maintaining diversity during a disease outbreak.
- Author
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McKnight DT, Lal MM, Bower DS, Schwarzkopf L, Alford RA, and Zenger KR
- Subjects
- Animals, Anura genetics, Genetic Variation, Geography, Polymorphism, Single Nucleotide genetics, Queensland, Sample Size, Anura microbiology, Biodiversity, Disease Outbreaks, Mycoses epidemiology, Refugium
- Abstract
Recent decades have seen the emergence and spread of numerous infectious diseases, often with severe negative consequences for wildlife populations. Nevertheless, many populations survive the initial outbreaks, and even undergo recoveries. Unfortunately, the long-term effects of these outbreaks on host population genetics are poorly understood; to increase this understanding, we examined the population genetics of two species of rainforest frogs (Litoria nannotis and Litoria serrata) that have largely recovered from a chytridiomycosis outbreak at two national parks in the Wet Tropics of northern Australia. At the wetter, northern park there was little evidence of decreased genetic diversity in either species, and all of the sampled sites had high minor allele frequencies (mean MAF = 0.230-0.235), high heterozygosity (0.318-0.325), and few monomorphic markers (1.4%-4.0%); however, some recovered L. nannotis populations had low N
e values (59.3-683.8) compared to populations that did not decline during the outbreak (1,537.4-1,756.5). At the drier, southern park, both species exhibited lower diversity (mean MAF = 0.084-0.180; heterozygosity = 0.126-0.257; monomorphic markers = 3.7%-43.5%; Ne = 18.4-676.1). The diversity patterns in this park matched habitat patterns, with both species having higher diversity levels and fewer closely related individuals at sites with higher quality habitat. These patterns were more pronounced for L. nannotis, which has lower dispersal rates than L. serrata. These results suggest that refugia with high quality habitat are important for retaining genetic diversity during disease outbreaks, and that gene flow following disease outbreaks is important for re-establishing diversity in populations where it was reduced., (© 2019 John Wiley & Sons Ltd.)- Published
- 2019
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12. Condition-dependent reproductive effort in frogs infected by a widespread pathogen.
- Author
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Roznik EA, Sapsford SJ, Pike DA, Schwarzkopf L, and Alford RA
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- Animals, Male, Queensland, Reproduction, Seasons, Anura microbiology, Anura physiology, Chytridiomycota physiology, Mycoses microbiology
- Abstract
To minimize the negative effects of an infection on fitness, hosts can respond adaptively by altering their reproductive effort or by adjusting their timing of reproduction. We studied effects of the pathogenic fungus Batrachochytrium dendrobatidis on the probability of calling in a stream-breeding rainforest frog (Litoria rheocola). In uninfected frogs, calling probability was relatively constant across seasons and body conditions, but in infected frogs, calling probability differed among seasons (lowest in winter, highest in summer) and was strongly and positively related to body condition. Infected frogs in poor condition were up to 40% less likely to call than uninfected frogs, whereas infected frogs in good condition were up to 30% more likely to call than uninfected frogs. Our results suggest that frogs employed a pre-existing, plastic, life-history strategy in response to infection, which may have complex evolutionary implications. If infected males in good condition reproduce at rates equal to or greater than those of uninfected males, selection on factors affecting disease susceptibility may be minimal. However, because reproductive effort in infected males is positively related to body condition, there may be selection on mechanisms that limit the negative effects of infections on hosts., (© 2015 The Author(s) Published by the Royal Society. All rights reserved.)
- Published
- 2015
- Full Text
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