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Background: The vitality of developing a safety culture in healthcare settings has become increasingly important following international investigations that highlighted the failures in health care delivery. Nurses, being at the frontline of healthcare, play a vital role in promoting patient safety and maintaining safety standards by being active in reducing medical errors. Weak patient safety culture has been identified as one of the major contributing factors to adverse events. Objectives: The present case investigated the different perceptions around patient safety culture and the factors considered of utmost importance to developing and maintaining this culture among nurses residing and working in Qatar. Methods: The present study utilized the English version of the Hospital Survey on Patient Safety Culture (HSOPSC) to collect responses from nurses residing and working in Qatar to determine their perceptions of patient safety culture. A convenient sample from the conference delegates of Middle East Forum for Quality and Safety 2018 was used as participants. Participation in the self-reporting survey was completely voluntary and anonymous. Results: The highest rated culture dimensions were organizational learning, continuous improvement, and teamwork within hospital units (89% and 88% positive responses, respectively). The lowest rated dimensions were non-punitive responses to error and staffing issues (28% and 35% positive responses, respectively). Conclusions/Implications for Practice: Similar to the global trends, error reporting should be viewed as a strategy to learn from mistakes and an initial step to create patient safety culture. In Qatar, while patient safety culture is generally well executed, with overall positive responses for the different measured composites, patient safety culture is yet to be fully developed. Initiatives are needed to improve staffing, handoffs, and transitions, as well as non-punitive responses to medical errors. [ABSTRACT FROM AUTHOR]