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Vascular endothelial growth factor (VEGF) and fibroblast growth factor (FGF) signaling pathways play important roles in the formation of the blood vascular system and nervous system across animal phyla. We have earlier reported VEGF and FGF from Hydra vulgaris Ind-Pune, a cnidarian with a defined body axis, an organized nervous system and a remarkable ability of regeneration. We have now identified three more components of VEGF and FGF signaling pathways from hydra. These include FGF-1 , FGF receptor 1 (FGFR-1) and VEGF receptor 2 (VEGFR-2) with a view to deciphering their possible roles in regeneration. In silico analysis of proteins was performed using Clustal omega, Swiss model, MEGA 7.0, etc. Gene expression was studied by whole mount in situ hybridization. VEGF and FGF signaling was inhibited using specific pharmacological inhibitors and their effects on head regeneration were studied. Expression patterns of the genes indicate a possible interaction between FGF-1 and FGFR-1 and also VEGF and VEGFR-2. Upon treatment of decapitated hydra with pharmacological inhibitor of FGFR-1 or VEGFR-2 for 48 h, head regeneration was delayed in treated as compared to untreated, control regenerates. When we studied the expression of head specific genes HyBra1 and HyKs1 and tentacle specific gene HyAlx in control and treated regenerates using whole mount in situ hybridization, expression of all the three genes was found to be adversely affected in treated regenerates. The results suggest that VEGF and FGF signaling play important roles in regeneration of hypostome and tentacles in hydra. • In silico analysis of FGF-1, FGFR-1 and VEGFR-2 proteins from Hydra and their comparison with vertebrate counterparts. • The comparison predicts close functional similarity between them. • Inhibitors of VEGFR and FGFR adversely affect head regeneration in hydra. • The results point towards essential roles of VEGF and FGF signaling during the head regeneration process. • This study may provide insights into regeneration across metazoans since signaling and patterning mechanisms are conserved. [ABSTRACT FROM AUTHOR]