1. Cryopreservation of spermatozoa of the terrestrial Puerto Rican frog, Eleutherodactylus coqui.
- Author
-
Michael SF and Jones C
- Subjects
- Animals, Anura, Cell Membrane Permeability, Cryoprotective Agents, Fluorescent Dyes, In Vitro Techniques, Male, Puerto Rico, Solutions, Sperm Motility, Cryopreservation methods, Semen Preservation methods, Spermatozoa physiology
- Abstract
World-wide reports of amphibian population declines have led to increased interest in the reproductive biology of anurans. As a model system, here we present evidence for the effective cryoprotection of sperm from the Puerto Rican frog, Eleutherodactylus coqui, using mixtures of fetal bovine serum (FBS) and dimethylsulfoxide (Me(2)SO), glycerol or sucrose extenders. Using a fluorescent dye exclusion assay, we found that 53.9 and 50.4% of all sperm with intact membranes prior to freezing maintained membrane integrity after rapid freezing and thawing when protected with either a FBS/glycerol or FBS/sucrose solution, respectively. The methods reported here may be useful for similar work with many of the more than 700 other species in this genus.
- Published
- 2004
- Full Text
- View/download PDF