1. Genotypic diversity of clinical Coxiella burnetii isolates from Portugal based on MST and MLVA typing.
- Author
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Santos AS, Tilburg JJ, Botelho A, Barahona MJ, Núncio MS, Nabuurs-Franssen MH, and Klaassen CH
- Subjects
- Animals, Bacterial Typing Techniques methods, Coxiella burnetii classification, DNA, Bacterial analysis, DNA, Bacterial genetics, DNA, Ribosomal Spacer analysis, DNA, Ribosomal Spacer genetics, Genotype, Genotyping Techniques, Goats microbiology, Humans, Minisatellite Repeats, Portugal, Q Fever blood, Q Fever microbiology, Stillbirth veterinary, Coxiella burnetii genetics, Coxiella burnetii isolation & purification, Genetic Variation, Multilocus Sequence Typing methods
- Abstract
The temporal and spatial diversity of Coxiella burnetii genotypes associated with human and animal disease in Portugal was analysed using a 6-locus multiple-locus variable-number tandem repeat analysis (MLVA) and a 10-locus multi-spacer sequence typing (MST) panel. Fifteen cultured C. burnetii isolates from 13 Q fever patients and a stillborn goat and 6 additional PCR-positive ruminant tissue samples obtained during 2006-2011 were included in this study. Seven MLVA genotypes (types S-Y) were obtained, including 4 new MLVA types (U, V, W, and X), all corresponding to 3 MST profiles (types 4, 8, and 13) previously reported from France and Spain. MLVA types U-Y, all belonging to MST type 4, were found in acute Q fever patients from the districts of Évora, Faro, Lisbon, and Setúbal. Different MLVA types were associated with goats from Castelo Branco district (S) and chronic Q fever patients from both Castelo Branco and Lisboa districts (S and T), matching with MST types 13 and 8, respectively. In conclusion, a genotypic diversity of C. burnetii consistent with a non-outbreak situation was identified. The involvement of different genotypes in acute and chronic Q fever was found, linking one of the chronic genotypes to goats from the eastern region of the country., (Copyright © 2012 Elsevier GmbH. All rights reserved.)
- Published
- 2012
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