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1. Niche theory, neutral theory, and more recently the unifying theory of biodiversity offer alternative views of the structure (or not) of ecological communities. The relative importance of these theories has not been so thoroughly used to explain community assembly processes in phytophagous insects. 2. We studied the mechanisms of community assembly in pine trees phloem feeders, exploring the relative contributions of deterministic and stochastic processes. 3. Temporal and spatial partitioning of wood borers and bark beetles belonging to the pine phloem feeding gild, was assessed through flight traps and pine bolts extracted from felled live Pinus pinaster trees presenting evidence of decline/wilting. 4. We found that deterministic factors explained most cases of coexistence between species, with partitioning in space and time reducing asymmetric competition for resources and intraguild predation, mediated by differences in size. However, in the case of very closely related species with minimum differences in size—in this case three species of Arhopalus—they occupied the same niche in space and time, suggesting that stochastic processes are more likely to maintain co‐occurrence. 5. The present work indicates that both niche and stochastic processes contribute to the assembly of the phloem feeding community. This could be one more example of accordance with the unifying theory of biodiversity. [ABSTRACT FROM AUTHOR]

Bursaphelenchus xylophilus, the pinewood nematode and causal agent of pine wilt disease (PWD), is a globally destructive pine pest. PWD is favoured by high temperatures and drought, but B. xylophilus can also persist in symptomless hosts in areas not prone to PWD. We compared seedlings of different Pinus species that were inoculated with B. xylophilus when well‐watered and under moderate temperatures, conditions that favour sublethal infections. We compared isolates of B. xylophilus in south‐west Europe (Portugal) and south‐east USA (Louisiana). P. pinea, P. pinaster, and P. radiata were challenged in both areas with inoculations of local B. xylophilus. P. sylvestris in Portugal and P. palustris in Louisiana were also tested. Seedling mortality was low, as expected, but nematode establishment was common. There were higher nematode densities and more damage in seedlings of P. sylvestris and P. radiata, followed by P. pinaster, with P. pinea and P. palustris proving to be more tolerant. Life history of hosts was a better predictor of resistance to B. xylophilus than geographical origin, even under sublethal conditions. Fast‐growing species (P. radiata and P. pinaster) appeared most likely to be symptomless carriers in areas not prone to the development of PWD. Cold‐adapted species (P. sylvestris) may still be suitable hosts for the pathogen, even at the sublethal conditions of their distribution areas. There were more nematodes per gram of host using nematode isolates from Portugal. Nonetheless, our results opposed the hypothesis that North American pinewood nematodes have such low virulence that they are obligatorily saprophytic. [ABSTRACT FROM AUTHOR]

1. In pine ecosystems, the role of the larval nests of the Mediterranean defoliator Thaumetopoea pityocampa as shelter of other arthropods, was studied for the first time. In Portugal, Pinus stands which differed in understorey plant diversity and level of attack by T. pityocampa were compared. 2. The arthropod fauna found sheltering inside the nests consisted of 60 species, representing 12 foraging types. Both arthropod richness and abundance were positively correlated with nest size, expressed as larval biomass. Arthropod richness was further positively correlated with understorey plant diversity, while no correlation was detected between arthropod richness and nest density. 3. Spiders accounted for up to 50% of the species richness, while 80% of the individuals collected were either juveniles, or females with brood, implying that T. pityocampa nests are used for overwintering and brood care. Seventy-six per cent of the Araneae were nocturnal ground foragers and specialised cryptic hunters, demonstrating the occurrence of indirect interactions among species belonging to different communities, namely ground vegetation layers and forest pine coppice. 4. It was concluded that, in Mediterranean pine ecosystems, T. pityocampa nests (i) create habitat diversification and contribute to improve overwintering survival of a wide range of arthropod species; (ii) play a functional role in the food web of pine ecosystems, by promoting indirectly mediated interactions among species from different communities; and (iii) do not contribute to create habitat for symtopic phytophagous arthropods, since contrary to literature references for different types of shelters, 98% of the associated species belonged to foraging types other than T. pityocampa. [ABSTRACT FROM AUTHOR]

• It was evaluated the effect of defoliation and climate on pine trees growth. • Precipitation and temperature have a positive effect on tree growth. • Defoliation and droughts decrease growth but do not act synergistically. • Defoliated trees are less affected by droughts than non-defoliated trees. • Defoliated trees are not able to take advantage of favourable climate. Forests are widely affected by disturbances, both abiotic, climate related, and biotic, like insect outbreaks. However, the impact of the interaction of these disturbances on tree growth is still not well understood. On the present work the impact of climatic and long-term defoliation by Thaumetopoea pityocampa (Dennis and Schiff.) during summer on Pinus pinaster growth was evaluated. At the National Pine Forest of Leiria, in Portugal, we sampled defoliated and non-defoliated forest plots covered by monocultures of young P. pinaster trees. Growth was assessed using dendrochronological techniques, and its relationship with climatic variability, including severe droughts, and defoliation, was analysed using hierarchical partitioning models. Results suggest that long term defoliation result in trees not being able to take advantage of favourable climatic conditions, leading to a reduced radial and height growth. Droughts were the major climatic disturbance, causing growth reduction as well, although the impacts of both disturbances did not act synergistically. Additionally, models showed that previous year climatic conditions have a significant effect on annual growth, namely, previous year annual precipitation and temperature. Yet, the best model obtained include a positive effect of the previous year annual precipitation and a negative effect of the interaction between this climatic parameter and defoliation. This work illustrates the potential of defoliation and climate to reduce forest productivity and the importance of water availability for tree development in Mediterranean climates. In addition, our results also emphasise the need to simultaneously evaluate the effects of different disturbances, particularly in a time of global change when an increase in forests disturbances is expected. This approach will certainly contribute to a more informed management of production forests. [ABSTRACT FROM AUTHOR]

The Neves-Corvo mining complex (MC) situated in southern Portugal exploits one of the most world’s important copper deposits. Agricultural soils surrounding the MC, used by the inhabitants for crop production, contain excessive amounts of As, Cu, Pb, and Zn. Thus, a potential risk to human consumption exists if edible plants grow on these substrata. Arsenic and Pb were not detected in edible samples collected near the MC and 5 km away, but in the leaves—structural or adsorbed onto the surface. In general, Zn was the most mobile element in both contaminated and reference areas as seen by the bioaccumulation factors (BAF). The tolerable upper intake (TUI) values for Cu are a reason of concern, since in 57.1% of the cases, the TUI values are above the recommended upper limit of 5 mg/day, in the case of Ficus carica, Cucurbita pepo, and Phaseolus vulgaris, whereas in 28.6% of the cases, the TUI values are near this limit (C. pepo and Citrus x sinensis). The consumption of such vegetables from these areas must be banned or strongly reduced, since long-term accumulation of Cu can cause a chronic toxicity in humans. [ABSTRACT FROM AUTHOR]