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Stomatopods in India are well known with 79 species recorded to date. Here I report the Odontodactylus japonicus (De Haan, 1844) and Golden Mantis Shrimp Lysiosquilla tredecimdentata Holothuis, 1941 for the first time in Puducherry coastal waters. A single specimen of Lysiosquilla tredecimdentata was collected from by-catch in the Nallavadu landing centre, Puducherry coast on 19 November 2019 and two specimens of L. tredecimdentata were recorded again in Pillaichavadi landing centre of Puducherry coast on 22 November 2019. One specimen of Odontodactylus japonicus was collected at Nallavadu landing centre, Puducherry coast on 20 December 2019. The present study was undertaken to identify the status of distribution, habitat, and ecological aspects along with the information of spread, confinement, endemism as well as rare, threatened and endangered species. The significance of these new observations is to discern the taxonomic position and characteristics for better understanding of the mantis shrimp group. The specimens were identified, described, illustrated, and measured morphometrically. [ABSTRACT FROM AUTHOR]

A structured survey was conducted to assess the knowledge, awareness and trends in disposal of broiler slaughter waste in Puducherry. Total of 50 broiler retailers were selected randomly and information was collected by interviewing with a pre-tested questionnaire to assess their knowledge on meat borne diseases, awareness on good slaughter practices, methods of slaughter waste disposal and sanitary measures followed. The data collected was analyzed statistically by Chisquare test using the software graph pad prism to find out the relationship between education and other variables. Among the respondents only 38 percent had awareness on transmission of meat borne diseases. Around 90 per cent broiler retailers sanitized the place at the end of slaughter. Only 26 per cent of respondents were aware of proper slaughter waste disposal methods. Eighty two per cent of them disposed their effluent into public drainage and 18 per cent by letting it to flow into the ground. Among them 56 per cent disposed their solid waste into garbage bins, 32 per cent gave to the fish farmers and 12 per cent fed it to stray dogs. The study also revealed the willingness of the broiler retailers in adaptingan economical cum eco-friendly method of waste disposal. Two methods with minimum investment for the disposal of broiler slaughter waste were attempted in the laboratory with an aim of transfer of technology to the broiler retailers in Puducherry. Slaughter waste (gut, offals and feathers) was brought to laboratory and subjected to both aerobic and anaerobic composting. Based on the physical and chemical analysis, quality and time taken to convert the waste into compost, aerobic decomposition was found to be extremely viable. Moreover this can fetch additional income for the retailers by selling compost. [ABSTRACT FROM AUTHOR]

Abstract Biofilm are group of microbial community enclosed inside a well-organized complex structure made up of extracellular polymeric substances. Biofilm forming bacteria are the growing concern of morbidity and mortality in aquatic population. Among the different biofilm forming bacteria, Vibrio spp. are pathogenic, diverse and abundant group of aquatic organism. Aquatic animals like fish, mollusks and shrimp harbors pathogenic and non-pathogenic forms of Vibrio spp. In the present study, Vibrio strains were isolated from various region of Tamil Nadu (Chennai, Cuddalore and Tiruchirappalli) and Puducherry. A total of 155 strains were isolated from various sampling sites, among them 84 (54.7%) were confirmed as Vibrio spp. through biochemical characterization and Vibrio specific 16S rRNA gene amplification. Among the 84 strains 50 strains were confirmed as biofilm formers and they were categorized as strong (10), moderate (17) and weak (23), based on their biofilm forming ability. Further the pathogenicity of all the Vibrio strains were tested through lytic enzyme production (protease, gelatinase and hemolysin) and swarming motility. After 16S rRNA gene sequencing, the 10 strong biofilm forming strains were Vibrio alginolyticus (TFM17, TFM30, and UFM24), Vibrio parahaemolyticus (TFM1), Vibrio harveyi (CUS2), Vibrio spp. (CUS4) and Gammaproteobacteria (TFM4, TFM19, TFM61, CUS5). The present study is a first report dealing with the biofilm forming Vibrio spp. isolation from south east coastal regions of Tamil Nadu and Puducherry. Further the informations in the present study will help in finding novel strategies to combat multi drug resistance biofilm formers in aquaculture industries. Graphical abstract fx1 Highlights • Dissemination of biofilm forming Vibrio spp. was scrutinized from collected fish samples. • Rapidly identification of Vibrio spp. through Vibrio specific 16S rRNA gene amplification. • Morphological of the biofilm was observed under light and confocal laser scanning microscope. • Predominant strains like V. alginolyticus , V. harveyi and V. parahaemolyticus were identified from the fish samples. • This is the first report of Vibrio spp. population in south east region of Tamil Nadu and Puducherry. [ABSTRACT FROM AUTHOR]