1. Salino-alkaline lime of anthropogenic origin a reservoir of diverse microbial communities.
- Author
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Kalwasińska A, Deja-Sikora E, Szabó A, Felföldi T, Kosobucki P, Brzezinska MS, and Walczak M
- Subjects
- Archaea classification, Archaea genetics, Archaea isolation & purification, Bacteria classification, Bacteria genetics, Bacteria isolation & purification, Extreme Environments, Poland, Ponds analysis, RNA, Ribosomal, 16S genetics, Sodium Chloride analysis, Biodiversity, Calcium Compounds, Microbiota genetics, Oxides, Ponds microbiology, Salinity, Sodium Hydroxide
- Abstract
This paper presents study on the microbiome of a unique extreme environment - saline and alkaline lime, a by-product of soda ash and table salt production in Janikowo, central Poland. High-throughput 16S rDNA amplicon sequencing was used to reveal the structure of bacterial and archaeal communities in the lime samples, taken from repository ponds differing in salinity (2.3-25.5% NaCl). Surprisingly abundant and diverse bacterial communities were discovered in this extreme environment. The most important geochemical drivers of the observed microbial diversity were salinity, calcium ions, nutrients, and water content. The bacterial and archaeal communities in saline, alkaline lime were similar to those found in natural haloalkaline environments. Although the archaeal contribution to the whole microbial community was lower than 4%, the four archaeal genera Natronomonas, Halorubrum, Halobellus, and Halapricum constituted the core microbiome of saline, alkaline lime - a set of OTUs (> 0.1% of total archaeal relative abundance) present in all samples under study. The high proportion of novel, unclassified archaeal and bacterial sequences (not identified at 97% similarity level) in the 16S rRNA gene libraries indicated that potentially new genera, especially within the class of Thermoplasmata inhabit this unique environment., (Copyright © 2018 Elsevier B.V. All rights reserved.)
- Published
- 2019
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