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The study was carried out on 24 athletes, who performed a 2000 m rowing ergometer test at the maximum rate and power of rowing. Before the exercise, one minute after its termination, and after the subsequent 24 hours, blood samples were drawn from the antecubital vein. The following parameters were evaluated in the blood: antioxidative enzymes activity (SOD, GPx) and thiobarbituric acid reactive substances (TBARS). Furthermore, the concentration of serum creatine kinase (CK) and Total Antioxidant Capacity (TAC) were assessed. The study results revealed that the rowers' peak exercise resulted in a significant increase in SOD, TAC and CK activity and free radical injury measured with TBARS concentration. During a 24-hour recovery period, none of the parameters evaluated returned to the rest values, which indicates persisting disturbances of the antioxidative defense mechanisms. The results of the study showed that intensive physical exercise increased free radical injury, in spite of an adaptive response of the antioxidative defense system. [ABSTRACT FROM AUTHOR]

Chlorfenvinphos is an organophosphate insecticide widely used alone or in combination in Poland. In the present study, the influence of chlorfenvinphos on the activity of mitochondrial antioxidative system as well as hydrogen peroxide concentration was examinated. The experiments were conducted on male Wistar rats of 180 ± 20g body weight. The animals were divided into two groups: the control group, which received oil intragastrically by stomach tube and the experimental groups, which received oil solution of chlorfenvinphos in doses of 0.02 LD50, 0.1 LD50 or 0.5 LD50. After 1, 24, 48 hours the livers were quickly removed. Liver mitochondria were isolated as described elsewhere. Glutathione peroxidase, superoxide dismutase, isocitrate dehydrogenase activity as well as reduced glutathione and hydrogen peroxide concentrations were determined in liver mitochondria using BIOXY-TECH Assay kits produced by OXIS International, Inc., Portland, USA. The results of this work indicate that chlorfenvinphos induces oxidative stress to rat liver mitochondria. In acute chlorfenvinphos intoxication, we demonstrated that the key role in the oxidative mitochondrial damage play MnSOD and GSH pool, as well as accumulation of hydrogen peroxide. [ABSTRACT FROM AUTHOR]