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Context:Perovskia atriplicifoliaBenth (Labiantae) has long been used as a traditional herbal medicine for anti-inflammation in Pakistan; this prompted us to isolate anti-inflammatory compounds from this plant. Objective: The objective of this study was to isolate and characterize the anti-inflammatory principles fromPerovskia atriplicifolia. Materials and methods: The CHCl3-soluble fraction of the methanol extract of the whole plant on column chromatography yielded compounds1–6. The anti-inflammatory potential of the compounds1–6was evaluated by Leukotriene C4 (LTC4) Release Assay which was performed according to the established protocol. LTC4in the supernatant of each well was measured using an ELISA kit (Cayman Chemical Co., Ann Arbor, MI). Results: The bioassay-guided phytochemical investigation of the CHCl3soluble fraction of the methanol extract ofPerovskia atriplicifoliafurnished six compounds, abrotanone (1), abrotandiol (2), (+)-pinoresinol (3), (+)-syringaresinol (4), (+)-lariciresinol (5), and (+)-taxiresinol (6). The compounds (1–6) were evaluated for their inhibitory activities on LTC4release. Among the tested compounds, (+)-taxiresinol (6) exhibited the most potent inhibition of LTC4release with an IC50value of 3.4 ± 0.09 µM followed by compounds4,5,3,and2with an IC50value ranging from 7.9 ± 0.04 to 17.2 ± 0.07 µM. Abrotanone (1) showed the lowest inhibition of LTC4release with an IC50value of 35.1 ± 0.05 µM (the positive control, zileuton, 0.77 ± 0.05 µM). Conclusion: Compounds1–6were found to possess inhibitory activity and seem to have potential therapeutic effect on inflammatory diseases. [ABSTRACT FROM PUBLISHER]

Abstract: Two new pentacyclic triterpenes named kirmanoic acid (1) and kurramanoic acid (2) have been isolated from the chloroform-soluble portion of the whole plant of Nepeta clarkei Hook. The structures of the two new compounds were assigned on the basis of their 1H and 13C NMR spectra including two-dimensional NMR techniques such as COSY, HMQC, and HMBC experiments. Kirmanoic acid (1) was investigated for analgesic, anti-inflammatory, and CNS depressant activities. Interestingly kirmanoic acid (1) showed strong analgesic activity than standard drug in acetic induced writhing and formalin tests. Similarly kirmanoic acid (1) also showed strong anti-inflammatory activity than its standard drug. The gross behavioral study of kirmanoic acid (1) revealed that it exhibited mild CNS stimulant and muscle relaxant in the mice. Compound 1 showed a slight increase in Locomotor activity and possesses the antidepressant effect [Copyright &y& Elsevier]

Vicarin (1), a new isoflavone, has been isolated from the ethyl acetate-soluble fraction of the ethanolic extract of Eremostachys vicaryi, along with soforanarin B (2), luteolin 7-O-β-d-glucopyranoside (3), and hamighriprasin (4). Their structures were elucidated on the basis of their spectral data including MS and 2D NMR. [ABSTRACT FROM PUBLISHER]

Two new phthalates bis(2-ethylundecyl)phthalate (1) and bis(2-ethyltridecyl)phthalate (2) have been isolated from the chloroform-soluble portion of the whole plant of Nepeta clarkei along with one known compound β-amyrin. The structures of the two new compounds and β-amyrin were assigned on the basis of their 1H and 13C NMR spectra including 2D NMR techniques such as COSY, HMQC, and HMBC experiments and compared with the literature data. [ABSTRACT FROM AUTHOR]