1. Mutation in the intracellular chloride channel CLCC1 associated with autosomal recessive retinitis pigmentosa.
- Author
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Li L, Jiao X, D'Atri I, Ono F, Nelson R, Chan CC, Nakaya N, Ma Z, Ma Y, Cai X, Zhang L, Lin S, Hameed A, Chioza BA, Hardy H, Arno G, Hull S, Khan MI, Fasham J, Harlalka GV, Michaelides M, Moore AT, Coban Akdemir ZH, Jhangiani S, Lupski JR, Cremers FPM, Qamar R, Salman A, Chilton J, Self J, Ayyagari R, Kabir F, Naeem MA, Ali M, Akram J, Sieving PA, Riazuddin S, Baple EL, Riazuddin SA, Crosby AH, and Hejtmancik JF
- Subjects
- Animals, Asian People genetics, Cell Line, Chloride Channels metabolism, Cytoplasm metabolism, Eye Proteins genetics, Eye Proteins metabolism, HEK293 Cells, Homozygote, Humans, Mice, Mice, Knockout, Pakistan, Retina metabolism, Retinal Cone Photoreceptor Cells metabolism, Retinal Rod Photoreceptor Cells metabolism, Retinitis Pigmentosa diagnosis, Zebrafish genetics, Zebrafish metabolism, Chloride Channels genetics, Mutation, Missense, Retinitis Pigmentosa genetics
- Abstract
We identified a homozygous missense alteration (c.75C>A, p.D25E) in CLCC1, encoding a presumptive intracellular chloride channel highly expressed in the retina, associated with autosomal recessive retinitis pigmentosa (arRP) in eight consanguineous families of Pakistani descent. The p.D25E alteration decreased CLCC1 channel function accompanied by accumulation of mutant protein in granules within the ER lumen, while siRNA knockdown of CLCC1 mRNA induced apoptosis in cultured ARPE-19 cells. TALEN KO in zebrafish was lethal 11 days post fertilization. The depressed electroretinogram (ERG) cone response and cone spectral sensitivity of 5 dpf KO zebrafish and reduced eye size, retinal thickness, and expression of rod and cone opsins could be rescued by injection of wild type CLCC1 mRNA. Clcc1+/- KO mice showed decreased ERGs and photoreceptor number. Together these results strongly suggest that intracellular chloride transport by CLCC1 is a critical process in maintaining retinal integrity, and CLCC1 is crucial for survival and function of retinal cells., Competing Interests: The authors have declared that no competing interests exist.
- Published
- 2018
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