1. Leishmanicidal active withanolides from a pakistani medicinal plant, Withania coagulans.
- Author
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Kuroyanagi M, Murata M, Nakane T, Shirota O, Sekita S, Fuchino H, and Shinwari ZK
- Subjects
- Antiprotozoal Agents isolation & purification, Antiprotozoal Agents pharmacology, Leishmania major drug effects, Magnetic Resonance Spectroscopy, Molecular Conformation, Pakistan, Plants, Medicinal chemistry, Withanolides isolation & purification, Withanolides pharmacology, Antiprotozoal Agents chemistry, Withania chemistry, Withanolides chemistry
- Abstract
In the course of screening for leishmanicidal constituents from Asian and South American medicinal plants, a Pakistani medicinal plant, Withania coagulans, showed activity. We therefore studied the active components of the methanol extract of aerial parts of W. coagulans. From the ethyl acetate soluble fraction of the extract, four new withanolides (1-4) were isolated along with seven known withanolides (5-11). The new compounds were elucidated to be (14R,15R,17S,20S,22R)-14,15,17,20-tetrahydroxy-1-oxowitha-2,5,24-trienolide (1), (14R,15R,17S,20S,22R)-14,15,17,20-tetrahydroxy-1-oxowitha-3,5,24-trienolide (2), (14S,17R,20S,22R)-14,17,20-trihydroxy-1-oxowitha-2,5,24-trienolide (3), and (14S,17R,20S,22R)-14,17,20-trihydroxy-1-oxowitha-3,5,24-trienolide (4), from 1H-NMR, 13C-NMR, 2D-NMR and high resolution (HR)-MS data. Some of these compounds having the partial structure 1-oxo-2,5-diene showed strong leishmanicidal activity against Leishmania major.
- Published
- 2012
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