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The aim of the present research was to investigate the presence of heavy metals such as lead (Pb), copper (Cu), chromium (Cr), and cadmium (Cd) in blood samples from cows raised with irrigated wastewater, as well as in the wastewater itself, in the North-western region of Pakistan. A total of 60 blood samples were collected from five different locations in Kohat, namely Tappi Road (TR), Pindi Road (PR), Gul Malik Road (GMR), Markaz Road (MR), and a control group. The samples of both i.e. cow blood and wastewater were analyzed for the concentrations of heavy metals. The highest concentration of Cd was detected in the MR site with a mean value of 0.03 mg/L, and the highest concentration of Cu (0.04 mg/L) was recorded in the TR site, while the lowest level was found in the control group with a mean of 0.002 mg/L in blood samples. The highest Cr and Pb concentrations were found at the PR site, with mean values of 0.03 and 0.07 mg/L, respectively, whereas the control group had the lowest concentrations, with mean values of 0.002 and 0.01 mg/L. Similarly, heavy metal concentrations were analyzed in wastewater used for irrigation in the study area. Results indicated elevated concentrations of Cu and Cr in wastewater, although they remained below the World Health Organization (WHO) recommended values except for Cr (0.13 mg/L) in the GMR site, which exceeded permissible limits. Cd and Pb concentrations in wastewater were relatively low, but Cd concentration surpassed WHO limits, particularly with a mean concentration of 0.08 mg/L in the TR site. Comparison between heavy metal concentrations in blood and wastewater revealed higher values of Cd and Pb in blood samples than in wastewater, while Cu and Cr concentrations were higher in water compared to blood. Additionally, elevated levels of Super Oxide Dismutase (SOD), antioxidant enzyme Catalase (CAT), and oxidative stress marker malondialdehyde (MDA) were detected in blood samples. Cluster and principal component analyses were employed to assess heavy metal toxicity among the groups, indicating potential long-term adverse health effects on animals, transfer to humans, and toxicity in living organisms. [ABSTRACT FROM AUTHOR]

Life cannot exist without water. Water scarcity is caused by massive groundwater decline. Water contamination is the most common problem spreading worldwide quicker than ever, alongwith anthropogenicwater scarcity. Since tainted water can harm health, water contamination is also contributing to water scarcity. Heavy metals in drinking water have plagued most Asian, African, and European nations for decades. Studies reveal that heavy metals have caused damage in Pakistan, India, and China. This study detects heavy metals in groundwater and treats them with phytoremediation along the river Kabul in district Charsadda. It also measures pH, EC, TDS, turbidity, fluoride, phosphates, nitrites, and nitrates. All indicatorsmeet the WHO and national environmental quality criteria for drinking water, except for turbidity, which exceeds the limit of =5 NTU in four examined regions, reaching a maximum of 9.99 NTU. Chemical parameters were within the standard limits, except for high concentrations of arsenic (As) and cadmium (Cd) in samples from S2 (15.20 µg/L) and S1 (20.50 µg/L) compared to WHO's 10 and 5 µg/L standards. However, the limit is within EPA Pakistan's 50 and 100 µg/L standards for drinking water, which Pakistan still follows. Heavymetals can harmhealth, even at lowlevels. Since themajority of the study area's population relies on groundwater for drinking and other needs, heavy metal pollution of the groundwater can cause many ailments. Thus, phytoremediation is increasingly vital to reduce these heavy metals to WHO limits to protect human health and the environment. [ABSTRACT FROM AUTHOR]

First-ever measurements of particulate matter (PM2.5, PM10, and TSP) along with gaseous pollutants (CO, NO2, and SO2) were performed from June 2019 to April 2020 in Faisalabad, Metropolitan, Pakistan, to assess their seasonal variations; Summer 2019, Autumn 2019, Winter 2019–2020, and Spring 2020. Pollutant measurements were carried out at 30 locations with a 3-km grid distance from the Sitara Chemical Industry in District Faisalabad to Bhianwala, Sargodha Road, Tehsil Lalian, District Chiniot. ArcGIS 10.8 was used to interpolate pollutant concentrations using the inverse distance weightage method. PM2.5, PM10, and TSP concentrations were highest in summer, and lowest in autumn or winter. CO, NO2, and SO2 concentrations were highest in summer or spring and lowest in winter. Seasonal average NO2 and SO2 concentrations exceeded WHO annual air quality guide values. For all 4 seasons, some sites had better air quality than others. Even in these cleaner sites air quality index (AQI) was unhealthy for sensitive groups and the less good sites showed Very critical AQI (> 500). Dust-bound carbon and sulfur contents were higher in spring (64 mg g−1) and summer (1.17 mg g−1) and lower in autumn (55 mg g−1) and winter (1.08 mg g−1). Venous blood analysis of 20 individuals showed cadmium and lead concentrations higher than WHO permissible limits. Those individuals exposed to direct roadside pollution for longer periods because of their occupation tended to show higher Pb and Cd blood concentrations. It is concluded that air quality along the roadside is extremely poor and potentially damaging to the health of exposed workers. [ABSTRACT FROM AUTHOR]

Environmental pollution as a result of industrial activity is widespread in many urban areas including Chicago. In an effort to evaluate the heavy metal fraction originating from industrial activities, plant samples of Daucus Carota or wild carrot were collected at or adjacent to six sites located in two of Chicago's designated industrial corridors. Plants, especially herbaceous species, have been deemed suitable as environmental pollution monitors as they are able to provide information about the heavy metal fraction accessible to biota. The leaves of Daucus Carota were acid digested and analyzed with total reflection X‐ray fluorescence spectrometry TXRF. The results showed elevated heavy metal mass fractions for at least one collection site which is close to an operational railyard. Other studies investigating heavy metals in proximity to railroad operations found elevated mass fractions for several elements, but specifically manganese as well. This suggests that abrasion from shunting and breaking releases certain pollutants into the local environment. The data were compared with studies executed in Rome, Italy, and Pakistan, which used Daucus Carota to evaluate heavy metal pollution. It was found that the heavy metal mass fractions obtained for Chicago were higher for some elements indicating an increased pollutant burden for these elements. The same samples were also analyzed by graphite furnace atomic absorption spectrometry GFAAS for the elements copper and lead and the data compared. Those two elements were chosen as they were present at each location and GFAAS has proven to be highly sensitive for them. It was found that the two methods provided comparable results for copper, whereas for lead, TXRF overestimated the mass fractions most likely due to limitations of the spectra evaluation software. The analysis of a certified reference material ‘BCR 679 white cabbage’ showed that most data obtained by TXRF were in good agreement with the certified values, with the exception of lead, which was not certified. However, since GFAAS has high sensitivity toward lead and is considered reference method for that element by regulatory agencies, a comparison between GFAAS and TXRF data for lead in the same sample can serve as good indicator for TXRF performance. [ABSTRACT FROM AUTHOR]

The current study worked on heavy metals concentrations in sediments of the alpine lakes in Gilgit-Baltistan, Northern Pakistan. Sediments samples (n = 41) from alpine lakes: Sadpara Lake, Rama Lake, Upper Kachura Lake, and Shausar Lake were collected. Sediments samples were digested according to standard protocol and analysed by atomic absorption spectrometry. Among heavy metals, Mn exhibited the highest mean concentration (333 mg/kg) in the sediments of alpine lakes, while Cd showed the lowest (1.48 mg/kg). The Ni, Cu, Cr, Mn, and Zn showed the highest mean concentrations in Sadpara Lake compared to other lakes. Sediments heavy metals concentrations were used for the calculation of pollution factors of alpine lakes, such as contamination factor (CF), pollution loads index (PLI), and ecological risk assessment (ERA). Among alpine lakes, the Sadpara Lake showed the highest PLI and CF values for most of the heavy metals. However, the highest mean (74.2 and 104) values of ERA and risk index (RI) were noted in the Rama Lake and exhibited a low-level risk to the alpine lake's system. Statistical analyses showed that weathering of bedrock and rich mineralised zone in the vicinity were dominating contributors of heavy metals contaminations. [ABSTRACT FROM AUTHOR]

Urbanization significantly contributes to soil contamination, and tourism-related activities may exacerbate the problem. Murree is a renowned tourist destination in Pakistan. In recent years, Murree's contamination levels have increased due to growing tourism and rapid urbanization. This study was designed to evaluate the contamination levels and measure the natural radioactivity in the urban soils of Murree. In this study, elemental analysis of soil samples from the urban areas of Murree was performed using Inductively Coupled Plasma-Optical Emission Spectroscopy (ICP-OES) and an elemental analyzer, while the activities of naturally occurring radionuclides (NORMs) were measured using Gamma Spectrometry.. The concentrations of 26 elements were measured, and Ca (47,761 mg/kg) was found to have the highest concentration and while Sn (3 mg/kg) had the lowest. Various parameters, such as Enrichment factor, Geo-accumulation index, Pollution and Integrated pollution index, and Ecological risk factor were calculated to assess the soil contamination levels. These parameters revealed low to moderate contamination at most of the sites and high contamination levels at one site. Principal Component Analysis (PCA) and correlation matrix revealed various sources for these metals. Non-carcinogenic and carcinogenic health hazards related to Cu, Pb, As, Ni, Cr, Mn, Ba, Zn and Co, exposure via three pathways (inhalation, dermal contact, and ingestion) were calculated for both adults and children; namely Average Daily Dose (ADD), Hazard Quotient (HQ), Hazard Index (HI) and Cancer Risk for Lifetime Exposure (CRLE). The highest HI value observed in adults was 0.023 for Ni and in children 0.207 for Co. Cr exhibited a carcinogenic risk for both adults and children, whereas As posed a cancer risk only to children. The average specific activities of Ra-226, Th-232, K-40 and Cs-137 in Bq/kg were 26.8 ± 14.4, 17.4 ± 5, 495.9 ± 82, 8 ± 3.2 respectively. Health risks associated with exposure to radiation from radionuclides were also assessed. The spatial distribution of heavy metals and NORMs were studied using interpolation to quantify their distribution geographically in Murree. This study concludes that some urban areas of Murree, near the city center are highly contaminated and the radiological risk to the population and environment is low. [ABSTRACT FROM AUTHOR]

Hindukush (HK), Karakoram (KK), and Himalayan (HM) ranges (collectively called HKH), an extension of the Tibetan plateau, are sensitive areas for mercury (Hg) and other trace metals (TMs) contamination. These metals reach remote regions via long-range atmospheric transport from distant transboundary pollution sources, whereas local emissions, physiography, and climatic properties of alpine regions cause further enrichment of Hg and other TMs. Little is known about the chemical cycling of Hg and other TMs in the HKH region, which was investigated in the current study. Sediment and peat cores were taken from 10 remote lakes of the region, comprising three sediments and one peat core each from HM and KK, and two sediment cores from HK region. The mean concentration of total Hg in HM lakes was 13.08 µg/g, 8.46 µg/g in HK lakes, and 4.65 µg/g in KK lakes. Other metals, including iron (Fe), manganese (Mn), nickel (Ni), cobalt (Co), chromium (Cr), zinc (Zn), cadmium (Cd), arsenic (As), and selenium (Se), were also investigated in these cores. The mean concentrations of these metals in all three ranges were observed to be in decreasing order of Fe > Mn > Zn > Ni > Cr > As > Pb > Se > Cd. Overall, HM lakes were found to be highly enriched in Hg and other TMs, compared to KK and HK lakes. Both the mass burial rate (MBR) and mass burial flux (MBF) of Hg and other TMs were in decreasing order of HM > KK > HK. As a result of these findings, Hg might pose a potential risk within the remote lakes of HKH; therefore, further studies are highly recommended to understand the geochemistry, source apportionment, and bioaccumulation of Hg and other toxic metals in this pristine region. [ABSTRACT FROM AUTHOR]

The heavy metal burden on natural freshwater ecosystems is uninterruptedly increasing, which could affect their biodiversity, particularly regarding avian species. Three river barrages were selected for the sampling of water birds from autumn 2021 to spring 2022. Seven heavy metals—nickel (Ni), copper (Cu), cobalt (Co), zinc (Zn), lead (Pb), cadmium (Cd), and manganese (Mn)—were estimated in the hearts and livers of Fulica atra (Common Coot), Anas strepera (Gad-wall), and Anas crecca (Eurasian Teal) (Linnaeus, 1758) by atomic absorption spectrometry. The mean concentrations of metals were found to be significantly (p < 0.05) different among the sam-pling sites, species, and tissues. In the livers of F. atra and A. strepera, respectively, the highest mean concentration among the metals belonged to Zn (521 µg/g), and the lowest belonged to Cd (0.17 µg/g). The concentrations of Zn, Cu, Pb, Cd, and Co were highest in A. strepera heart samples. However, the concentrations of Zn, Pb, Cd, and Mn were found to be highest in the livers of F. atra. A comparison between both tissues indicated that the concentration of Ni is significantly (p < 0.05) higher (except for F. atra from Trimmu barrage) in the livers of water birds and that the concentration of Cd is significantly higher in the hearts of water birds. The mean metal concentrations were higher than the background limits reported in various studies, suggesting that the wintering water birds of Pakistan are under a significant load of heavy metal pollution. Histopathological analysis suggested that the observed heavy metals altered the normal histologies of hearts and livers of Fulica atra (Common Coot), Anas strepera (Gadwall), and Anas crecca (Eurasian Teal) sampled from three wetlands of Punjab Pakistan. [ABSTRACT FROM AUTHOR]

The current study was conducted on the inhabitants living in the area adjacent to the Hudiara drain using bore water and vegetables adjacent to the Hudiara drain. Toxic heavy metals badly affect human health because of industrial environmental contamination. Particularly hundreds of millions of individuals globally have faced the consequences of consuming water and food tainted with pollutants. Concentrations of heavy metals in human blood were elevated in Hudiara drainings in Lahore city, Pakistan, due to highly polluted industrial effluents. The study determined the health effects of high levels of heavy metals (Cd, Cu, Zn, Fe, Pb, Ni, Hg, Cr) on residents of the Hudiara draining area, including serum MDA, 8-Isoprostane, 8-hydroxyguanosine, and creatinine levels. An absorption spectrophotometer was used to determine heavy metals in wate water, drinking water, soil, plants and human beings blood sampleas and ELISA kits were used to assess the level of 8-hydroxyguanosine, MDA, 8-Isoprostane in plasma serum creatinine level. Waste water samples, irrigation water samples, drinking water samples, Soil samples, Plants samples and blood specimens of adult of different weights and ages were collected from the polluted area of the Hudiara drain (Laloo and Mohanwal), and control samples were obtained from the unpolluted site Sheiikhpura, 60 km away from the site. Toxic heavy metals in blood damage the cell membrane and DNA structures, increasing the 8-hydroxyguanosine, MDA, creatinine, and 8-Isoprostane. Toxic metals contaminated bore water and vegetables, resulting in increased levels of creatinine, MDA, Isoprostane, and 8-hydroxy-2-guanosine in the blood of inhabitants from the adjacent area Hudiara drain compared to the control group. In addition,. This study also investigated heavy metal concentrations in meat and milk samples from buffaloes, cows, and goats. In meat, cow samples showed the highest Cd, Cu, Fe and Mn concentrations. In milk also, cows exhibited elevated Cu and Fe levels compared to goats. The results highlight species-specific variations in heavy metal accumulation, emphasizing the need for targeted monitoring to address potential health risks. The significant difference between the two groups i.e., the control group and the affected group, in all traits of the respondents (weight, age, heavy metal values MDA, 8-Isoprostane, 8-hydroxyguaniosine, and serum creatinine level). Pearson's correlation coefficient was calculated. The study has shown that the level of serum MDA, 8-Isoprostane, 8-hydroxyguaniosine, or creatinine has not significantly correlated with age, so it is independent of age. This study has proved that in Pakistan, the selected area of Lahore in the villages of Laloo and Mohanwal, excess of heavy metals in the human body damages the DNA and increases the level of 8-Isoprostane, MDA, creatinine, and 8-hydroxyguaniosine. As a result, National and international cooperation must take major steps to control exposure to heavy metals. [ABSTRACT FROM AUTHOR]

Soil pollution with heavy metals has grown to be a big hassle, leading to the loss in farming production particularly in developing countries like Pakistan, where no proper channel is present for irrigation and extraction of these toxic heavy metals. The present study aims to ameliorate the damages caused by heavy metal ions (Hg-Mercury) on rapeseed (Brassica napus L.) via a growth regulator (α-tocopherol 150 mg/L) and thermopriming technique at 4 °C and 50 °C to maintain plant agronomical and physiological characteristics. In pot experiments, we designed total of 11 treatments viz.(T0 (control), T1 (Hg4ppm), T2 (Hg8ppm), T3 (Hg4ppm + 4 °C), T4 (Hg4ppm + 4 °C + tocopherol (150 m/L)), T5 (Hg4ppm + 50 °C), T6 (Hg4ppm + 50 °C + tocopherol (150 mg/L)), T7 (Hg8ppm + 4 °C), T8 (Hg8ppm + 4 °C + tocopherol (150 mg/L)), T9 (Hg8ppm + 50 °C), T10 (Hg8ppm + 50 °C + tocopherol (150 mg/L) the results revealed that chlorophyll content at p < 0.05 with growth regulator and antioxidant enzymes such as catalase, peroxidase, and malondialdehyde enhanced up to the maximum level at T5 = Hg4ppm + 50 °C (50 °C thermopriming under 4 ppm mercuric chloride stress), suggesting that high temperature initiate the antioxidant system to reduce photosystem damage. However, protein, proline, superoxide dismutase at p < 0.05, and carotenoid, soluble sugar, and ascorbate peroxidase were increased non-significantly (p > 0.05) 50 °C thermopriming under 8 ppm high mercuric chloride stress (T9 = Hg8ppm + 50 °C) representing the tolerance of selected specie by synthesizing osmolytes to resist oxidation mechanism. Furthermore, reduction in % MC (moisture content) is easily improved with foliar application of α-tocopherol and 50 °C thermopriming and 4 ppm heavy metal stress at T6 = Hg4ppm + 50 °C + α-tocopherol (150 mg/L), with a remarkable increase in plant vigor and germination energy. It has resulted that the inhibitory effect of only lower concentration (4 ppm) of heavy metal stress was ameliorated by exogenous application of α-tocopherol and thermopriming technique by synthesizing high levels of proline and antioxidant activities in maintaining seedling growth and development on heavy metal contaminated soil. [ABSTRACT FROM AUTHOR]

The China-Pakistan Economic Corridor (CPEC) between China and Pakistan passes through the environmentally fragile region of the Himalayas−Karakorum−Hindukush (HKH). Surging human influence in the form of transportation and tourism may pose a potential threat to the socio-environmental sustainability especially, the water resources of the region. Therefore, it is important to evaluate the status and hydrogeochemistry of freshwater in mountainous catchments. For this purpose, water samples were collected from the Hunza, Gilgit, and Indus River Basins from 2019 to 2020. The physicochemical and biological characteristics of surface water were investigated using the water quality index (WQI), multivariate statistics, Piper diagram, and Gibbs plots. The results revealed high seasonal variability in the hydrochemistry of water samples in all basins. Heavy metals (HM) i.e., arsenic (As), and lead (Pb) were detected in more than 50% of total water samples, whereas cadmium (Cd) and mercury (Hg) were detected in less than 15% of total water samples. 82% of water samples collected during summer and 48% in winter were contaminated with bacterial contamination (Escherichia coli (E. coli) and total coliform (T. coli)). Piper diagram and Gibbs plot suggested that rock weathering and dissolution of carbonate rocks are the dominating factors controlling the chemistry of glacial streams and river water. The results of this study have important implications for policymakers, regulators, and practitioners involved in water and environmental management across CPEC. [ABSTRACT FROM AUTHOR]

This study sought to asses drinking and irrigation water quality in the Dera Ismail (DI) Khan District, Pakistan with the aid of using drinking water quality index (WQI) and irrigation water quality indices (IWQI). Seventy two water samples were collected from various water sources such as tube wells, dug wells, hand pumps, streams, and springs throughout the DI Khan district. The investigated physicochemical parameters include pH, TDS, EC, turbidity, total hardness, major cations (Na, K, Ca, Mg), anions (Cl, HCO3, SO4), and heavy metals (Fe, Mo, Cr, Ni, Cu, and Zn). IWQI used in the study includes Kelly’s ratio (KR), permeability index (PI), sodium percent (Na%), Magnesium Hazard (MH), potential salinity (PS), residual sodium carbonate (RSC), and sodium adsorption ratio (SAR). With the mean value of 63, WQI revealed that most samples are placed in the good category, with a smaller fraction in the excellent and poor classes. On the other hand, a health risk assessment of the data pointed out that the molybdenum (Mo) and chromium (Cr) concentrations in drinking water could pose health risks to children of the concerned population. Rock weathering appears to be the dominant hydro-geochemical pathway controlling water chemistry, Ca-Mg-HCO3 being the dominant facies, followed by Na-HCO3. Kelly’s ratio (KR), sodium percent (Na%), sodium adsorption ratio (SAR), and residual sodium carbonate (RSC) had values higher than the recommended threshold. The use of water for irrigation of agricultural fields may raise relevant ecological concerns in the studied district. [ABSTRACT FROM AUTHOR]

One of the primary environmental routes through which humans are exposed to metals and may be exposed to health risks is the food chain's contamination with heavy metals. The study observed the risks posed by contaminants in vegetables produced in soil that received wastewater irrigation, as well as their origins and the human health impacts. Eight harmful metals (Cu, Fe, Zn, Mn, Pb, Cd, Ni, and Cr) were tested for concentration levels in water, soil, and vegetable samples using analytical techniques and an atomic absorption spectrophotometer. The present study investigated the potential health implications associated with the consumption of vegetables irrigated using wastewater containing heavy metals. The results indicated a notable accumulation of heavy metals in plant and soil samples obtained from Kirri Shamozai, Pakistan. In comparison to vegetables cultivated in soil irrigated with fresh water, the concentration levels of heavy metals in vegetables grown on soil irrigated with untreated wastewater were considerably higher at (P ≤ 0.001) and above the World Health Organization (WHO) recommended limits. The results showed that heavy metals had significantly accumulated in the soil and had permeated into the crops. Heavy metal concentrations in vegetables cultivated on land irrigated with wastewater were more significant than those grown on land irrigated with freshwater. They exceeded US EPA and World Health Organization (WHO) limits. PCA results for Pb, Cu, and Cr are the main issues impacting water quality and health hazards. The PCA results show that the soil has an extensive loading of heavy metals Cd, Ni, and Mn. [ABSTRACT FROM AUTHOR]

Background. Heavy metal pollution has become a global environmental issue. Heavy metals are contaminating the agro-soils, growing crops, and vegetables through different agricultural practices. In this study, besides the phytoremediation potential of maize, the role of chromium (Cr) and lead (Pb) on crop and soil health has been investigated. Methods. Two maize varieties, Pak-Afgoi and Neelem, were grown under varying concentrations of Cr (50-300 ppm) and Pb (30-300 ppm) and different growth parameters i.e., seed germination, leaf size/number, stem girth, plant height, biomass, chlorophyll content, relative growth rate (RGR), and net assimilation rate (NAR) were studied under Cr and Pb stress. Likewise, the effect of metals was also assessed on different soil characteristics including soil texture, pH, EC, soil organic matter, urease activity and nutrients. Results. Studied plant attributes were adversely affected by heavy metals toxicity. Affected values of RGR and NAR showed a linear correlation with affected growth and dry matter yield of maize. Heavy metals impacted different soil parameters including soil microbial performance and revealed a declining trend as compared to control soil. Maize varieties showed a significant phytoremediation potential i.e., uptake of Cr and Pb was 33% and 22% in Pak-Afgoi, while Neelem showed 38% and 24% at 300 ppm, respectively. Data regarding metal translocation factor (TF), bioaccumulation factor (ACF), and biomagnification ratio (BMR) significantly revealed the potential of maize varieties in the removal of Cr and Pb metals from affected soils. However, Cr-accumulation was higher in shoots, and Pb accumulated in plant roots showed a differential behavior of metal translocation and affinity with the varieties. These maize varieties may be recommended for general cultivation in the Cr and Pb-contaminated areas. [ABSTRACT FROM AUTHOR]

Sediments were considered a sink and potential source of heavy metals in the aquatic system. For this purpose, the present study examined surface sediments for spatial and temporal variation of heavy metals pollution and risk indices in the Gomal Zam Dam Basin (GZDB), Pakistan. Sediment samples (n = 20) were collected from the GZDB, i.e., Gomal Zam Dam, its inlets, and outlets in the winter and summer seasons of 2020, and examined for heavy metals such as zinc (Zn), nickel (Ni), manganese (Mn), lead (Pb), chromium (Cr), copper (Cu), iron (Fe), and cobalt (Co) concentrations. Among GZDB, results showed that the Zhob River Inlet had a higher levels of heavy metals in both seasons. The results revealed that pollution load index values were < 1, observing no pollution in the aquatic system. The risk indices values revealed that sampling sites showed no or very low risk during the summer, 84% of samples showed no or very low risk during the winter, and the rest noted with reasonable risks. Winter season showed higher average values of contamination and risk indices than summer. Statistical analyses revealed that the heavy metals contaminations were mainly due to geogenic sources of rock weathering and ore deposits, with minor contributions from anthropic activities. This study recommends regular monitoring of temporal studies on heavy metals contamination in the water of the GZDB. [ABSTRACT FROM AUTHOR]

The most recent research has shown that the saline soil in Hyderabad, Sindh, Pakistan (southwestern Pakistan), was contaminated with trace and toxic metal concentrations above background levels. Therefore, the potential environmental hazards associated with the availability of Cd, Co, Cr, Cu, Pb, and Zn in this environment are of high ecological value. The mean values for Cd, Co, Cr, Cu, Pb, and Zn were found in the range of 1.01–3.26, 18.6–29.1, 5.07–13.6, 122–191, 4.01–9.99, and 170–193 μg/g in wetland metal-contaminated saline soil, respectively. The validation of the method was carried out by analysis of certified reference material; Community Bureau of Reference-483 (BCR-483) showed the percentage recovery > 98% in each case. The contamination factor (Cf), potential ecological risk factor (PERI), toxic response factor (Trf), degree of contamination (Dc), and potential ecological risk index (RI) showed that BS1 and BS3 contain more contamination of heavy metals. According to environmental risk analysis for heavy elements studies, the metal-polluted area of Hyderabad, Sindh, Pakistan, poses a high to moderate ecological danger. [ABSTRACT FROM AUTHOR]

Water and soil polluted by heavy metals (HMs) are the primary problem due to rapidly increasing urbanization and industrialization. For the treatment of polluted soil, phytoremediation turns into a cost-effective and eco-friendly technique. The current research aimed to examine the load of pollution, specifically HMs, in sediment and wastewater (WW) of the GadoonAmazai Industrial Estate (GAIE), Pakistan and compare the ability of native grass species Cynodon dactylon and Digiteria sanguinalis for the phytoaccumulation of HMs. The industrially polluted soil was analysed for HMs using atomic absorption spectrophotometry (AAS) and compared with healthy soil (irrigated by freshwater), which served as a control. The HM accumulation was considerably higher in the soil irrigated with WW than in control soil samples. The most substantial metal pollutant was manganese (Mn), which accumulated up to 2491.7 mg/kg in the WW irrigated soil. For assessing the bioremoval efficiency of grass species, pot experimentation was performed for 90 days. Soil samples and grasses were collected from the pots to examine the HM removal efficiency. A significant reduction was noted in physicochemical characteristics of the soil, such as electrical conductivity, total organic matter, phosphorus, potassium, and saturation. The grasses removed up to 59.0% of the Cu and 59.9% of Mn from the soil. The highest bioconcentration factor (BCF) and translocation factor (TF) of Cu were observed for D. sanguinalis. While the highest BCF and TF of Mn were obtained for C. dactylon. The research showed that the grass significantly (p ≤ 0.05) reduced HM in soil samples. Moreover, the selected grasses found a higher capability to accumulate HM in the roots than in the shoot. The maximum Cu removal was obtained by D. sanguinalis and Mn by C. dactylon. The research study concluded that phytoremediation using D. sanguinalis and C. dactylon is an eco-friendly and cost-effective method that can be utilized for soil remediation. [ABSTRACT FROM AUTHOR]

The present study was conducted on Head Punjnad (HP) and Head Taunsa (HT) to evaluate the contamination of Pb, Cr, As, Hg, and Cd in water, soil, sediment, fish as a whole and fish organs. Fish, water, soil and sediment samples were collected from different sites of HT and HP on a monthly basis for 8 months. Heavy metals in water, soil, and sediment were determined by a polarized Zeeman atomic absorption spectrophotometer and in fish and fish organs by an atomic absorption spectrophotometer. Contamination of Cd, Hg, and As was significantly (P<0.05) higher in water of HP as compared to HT, while Cr showed a non-significant (P>0.05) difference at HP and HT. Pb was significantly (P<0.05) higher in water of HT as compared to HP. In the case of soil, Cd, Hg, and Pb were higher at HT as compared to HP, while As and Cr were significantly (P<0.05) higher at HP as compared to HT. In sediment, contamination of Cd, Hg, and As were significantly (P<0.05) higher at HP as compared to HT, while the Cr difference was non-significant (P>0.05) but Pb showed a significantly (P<0.05) higher value at HT than HP. Cd accumulation in different fish species was recorded as R. rita ˃O. niloticus ˃C. marulius ˃S. sarwari ˃C. idella ˃C. catla ˃N. notopterus ˃E. vacha ˃L. rohita ˃C. carpio, respectively. Hg as O. niloticus ˃S. sarwari ˃R. rita ˃C. marulius ˃C. catla ˃N. notopterus ˃E. vacha ˃L. rohita ˃C. carpio ˃C. idella, respectively. As as O. niloticus ˃R. rita ˃S. sarwari ˃C. marulius ˃C. catla ˃C. carpio ˃N. notopterus ˃C. idella ˃E. vacha ˃L. rohita, respectively. Cr accumulation recorded as L. rohita ˃C. idella ˃O. niloticus ˃C. marulius ˃E. vacha ˃R. rita ˃C. catla ˃C. carpio ˃S. sarwari ˃N. notopterus, respectively. Pb accumulation in different fish species was recorded as C. idella ˃C. carpio ˃N. notopterus ˃L. rohita ˃O. niloticus ˃C. marulius ˃R. rita ˃S. sarwari ˃E. vacha ˃C. catla, respectively. Cd accumulation in different organs was recorded as kidney ˃liver ˃gills ˃muscle ˃skin ˃scale. Hg accumulation in different organs was recorded as kidney ˃gills ˃liver ˃skin ˃muscle ˃scale. As accumulation in different organs was recorded as kidney ˃liver ˃gills ˃muscle ˃skin ˃scale. Cr accumulation in different organs was recorded as gills ˃ liver ˃skin ˃muscle ˃kidney ˃scale. Pb accumulation in different organs was recorded as gills˃ kidney˃ skin˃ liver˃ muscle˃ scale. [ABSTRACT FROM AUTHOR]

Pakistan is the fourth largest yarn producer in the world heavily that relies on cotton crop which receives a substantial 62% of all pesticide applications. The present study was conducted to quantify the levels of pesticides such as bifenthrin, spirotetramat, pyriproxyfen, imidacloprid, and diafenthiuron in soil and plants residue at selected cotton fields of Multan District, Pakistan. In addition to pesticides, the assessment of heavy metal concentration was also conducted in order to determine the overall risks that these compounds to both plants and human population. For this analysis, 20 soil samples and 10 plant samples were collected from 10 selected cotton fields. Pesticides and heavy metals in soil and plant samples were analyzed using high-performance liquid chromatography (HPLC) and Inductively Coupled Plasma-Optical Emission Spectroscopy (ICP-OES), respectively. It was observed that all samples collected from selected fields contained pesticide residue in top soil (0–15 cm). However, no pesticides were detected in the lower soil layer (16–30 cm). In case of heavy metals, the highest concentration of Fe, Pb, and Mn was observed in both soil and plant residue samples. The heavy metals were found in the order of Fe > Mn > Pb > Zn > Cu > Cd in the soil. The total carcinogenic risk values for a few pesticides were found to range from 10−6 to 10−2, indicating that residents of the study area have low to higher chances of developing cancer. A positive correlation was observed among the pesticides (r = 0.18–0.95) as well as in metals related parameters (r = 0.49–0.96), where a weak negative correlation was found among metal to pesticide parameters except Pd where the maximum r value was 0.62. In general, the finding of this study encourages the development and adoption of sustainable agricultural practices that lower the dependence on toxic pesticides and endorse environmentally friendly alternatives. [ABSTRACT FROM AUTHOR]

Heavy metals are environmental pollutants and carcinogenic for human health if ingested. In developing countries, including Pakistan, untreated sewerage water is one of the major sources of irrigation for vegetable production in the vicinities of urban areas which might be toxic to human health due to heavy metals contamination. The present study was conducted to investigate the uptake of heavy metals by sewage water application and its impact on human health. The experiment consisted of five vegetable crops (Raphanus sativus L, Daucus carota, Brassica rapa, Spinacia oleracea, and Trigonella foenum-graecum L.) and two irrigation sources (clean water irrigation and sewage water irrigation). Each treatment was three time replicated for all five vegetables, and standard agronomic practices were applied. The results demonstrated that shoot and root growth in radish, carrot, turnip, spinach, and fenugreek was enhanced significantly with sewerage water, probably due to enhanced organic matter. However, pithiness was observed in the root of radish under sewerage water treatment. Very high concentrations of Cd, up to 7.08 ppm in turnip roots while up to 5.10 ppm in fenugreek shoot, were observed, and other vegetables also contained higher concentrations of Cd. Zn concentrations in the edible parts of carrot (control (C) = 129.17 ppm, sewerage (S) = 164.10 ppm), radish (C = 173.73 ppm, S = 253.03), turnip (C = 109.77 ppm, S = 149.67 ppm), and fenugreek (C = 131.87 ppm, S = 186.36 ppm) were increased by sewerage water treatment but a decrease in Zn concentration in spinach (C = 262.17 ppm, S = 226.97 ppm) was observed. Fe concentration in edible parts of carrot (C = 888.00 ppm, S = 524.80 ppm), radish (C = 139.69 ppm, S = 123.60 ppm), turnip (C = 195.00 ppm, S = 121.37 ppm), and fenugreek (C = 1054.93 ppm, S = 461.77 ppm) were also decreased by sewerage water treatment while spinach leaves had accumulated higher Fe (C = 1560.33 ppm, S = 1682.67 ppm) in sewerage water treatment. The highest bioaccumulation factor value was 4.17 for Cd in carrots irrigated with sewerage water. The maximum value of bioconcentration factor was 3.11 for Cd in turnip under control, and the highest value of translocation factor was 4.82 in fenugreek irrigated with sewerage water. Daily intake of metals and health risk index (HRI) calculation indicated that HRI for Cd was more than 1, suggesting toxicity in these vegetables while HRI for Fe and Zn is still under safe limit. Correlation analysis among different traits of all vegetables under both treatments revealed valuable information for selecting traits in the next crop breeding programs. It is concluded that untreated sewerage-irrigated vegetables, highly contaminated with Cd, are potentially toxic for human consumption and should be banned in Pakistan. Furthermore, it is suggested that the sewerage water should be treated to eliminate toxic compounds, particularly Cd, before irrigation usage and non-edible/phytoremediation crops might be grown in contaminated soils. [ABSTRACT FROM AUTHOR]

Heavy metals (HMs) are extensively found in occupationally exposed miners and industrial workers, which may cause serious health-related problems to the large workforce. In order to evaluate the impact of these toxic pollutants, we have investigated the effect of cadmium (Cd), chromium (Cr), copper (Cu), and lead (Pb) concentration on exposed workers of mining, and woolen textile mill and compared the findings with unexposed individuals. From each category like exposed workers (mining, and woolen mill textile site) and unexposed individuals, 50 blood samples were taken. The occurrence of HMs in a sample was investigated through atomic absorption spectrometry while the oxidative stress marker malondialdehyde (MDA) and antioxidant enzyme statuses such as superoxide dismutase (SOD) and catalase (CAT) were analyzed in exposed and control samples. The results showed significant (p < 0.05) variation in Cd, Cr, Cu, and Pb levels in exposed and control samples. The concentration of Cd in the blood of WMWs, KMWs, and control group was 5.75, 3.89, and 0.42 μg/dL, respectively. On the other hand, the concentration of Pb in the blood of WMWs, MWs, and control was 32.34, 24.39, and 0.39 µg/dL while the concentrations of Cr and Cu in the blood of WMWs, MWs, and control group were 11.61 and 104.14 μg/dL, 4.21 and 113.21 μg/dL, 0.32 and 65.53 μg/dL, respectively. An increase in MDA was recorded in the exposed workers' group as compared to control subjects, whereas SOD and CAT activities decreased. Meanwhile, MDA was significantly and positively (p < 0.01) correlated with HMs, while negative significant correlations were found among HMs with SOD and CAT. [ABSTRACT FROM AUTHOR]

Freshwater shortage and its contamination with various types of pollutants are becoming the most alarming issues worldwide due to impacts on socioeconomic values. Considering an increasing freshwater scarcity, it is imperative for the growers, particularly in semiarid and arid areas, to use wastewater for crop production. Wastewaters generally contain numerous essential inorganic and organic nutrients which are considered necessary for plant metabolism. Besides, this practice provokes various hygienic, ecological and health concerns due to the occurrence of toxic substances such as heavy metals. Pakistan nowadays faces a severe freshwater scarcity. Consequently, untreated wastewater is used routinely in the agriculture sector. In this review, we have highlighted the negative and positive affectivity of wastewater on the chemical characteristics of the soil. This review critically delineates toxic metal accumulation in soil and their possible soil–plant–human transfer. We have also estimated and deliberated possible health hazards linked with the utilization of untreated city waste effluents for the cultivation of food/vegetable crops. Moreover, we carried out a multivariate analysis of data (144 studies of wastewater crop irrigation in Pakistan) to trace out common trends in published data. We have also compared the limit values of toxic metals in irrigation water, soil and plants. Furthermore, some viable solutions and future viewpoints are anticipated taking into account the on-ground situation in Pakistan—such as planning and sanitary matters, remedial/management technologies, awareness among local habitants (especially farmers) and the role of the government, NGOs and pertinent stakeholders. The data are supported by 13 tables and 7 figures. [ABSTRACT FROM AUTHOR]

Water, forages, and soil contamination with potentially toxic metals (PTMs) through anthropogenic activities has become a significant environmental concern. It is crucial to find out the level of PTMs in water, soil, and forages near industrial areas. The PTMs enter the body of living organisms through these sources and have become a potential risk for humans and animals. Therefore, the present study aims at the health risk assessment of PTMs and their accumulation in soil, water, and forages of three tehsils (Kallar Kahar, Choa Saidan Shah, and Chakwal) in district Chakwal. Samples of wastewater, soil, and forages were collected from various sites of district Chakwal. PTMs detected in the present study were cadmium (Cd), chromium (Cr), lead (Pb), zinc (Zn), cobalt (Co), copper (Cu), and nickel (Ni), and their levels were measured through atomic absorption spectrophotometer (AAs GF95 graphite furnace auto sampler). Pollution load index (PLI), bio concentration factor (BCF), soil enrichment factors (EF), daily intake value (DIM), and health risk index (HRI) in sheep, cow, and buffalo were also analyzed. The results revealed that the mean concentration (mg/L) of Cd (0.72–0.91 mg/L), Cr (1.84–2.23 mg/L), Pb (0.95–3.22 mg/L), Co (0.74–2.93 mg/L), Cu (0.84–1.96 mg/L), and Ni (1.39–4.39 mg/L) in wastewater samples was higher than permissible limits set by WHO, NEQS, WWF, USEPA, and Pakistan in all three tehsils of district Chakwal. Similarly, in soil samples, concentrations of Cd (1.21–1.95 mg/kg), Cr (38.1–56.4 mg/kg), and Ni (28.3–55.9 mg/kg) were higher than their respective threshold values. The mean concentration of PTMs in forage samples (Parthenium hysterophorus, Mentha spicata, Justicia adhatoda, Calotropis procera, Xanthium strumarium, Amaranthaceae sp.) showed that maximum values of Cd (5.35–7.55 mg/kg), Cr (5.47–7.51 mg/kg), Pb (30–36 mg/kg), and Ni (12.6–57.5 mg/kg) were beyond their safe limit set for forages. PLI, BCF, and EF were > 1.0 for almost all the PTMs. The DIM and HRI for sheep were less than < 1.0 but for cows and buffalo were > 1.0. The current study showed that soil, water, and forages near coal mines area are contaminated with PTMs which enter the food chain and pose significant harm to humans and animals. In order to prevent their dangerous concentration in the food chain, regular assessment of PTMs present in soil, forages, irrigating water, and food is recommended. [ABSTRACT FROM AUTHOR]

Groundwater quality evaluation is the main concern in the regions like Chakwal where it is major source of water for drinking and irrigation due to low storage capacity of the surface water and lack of proper irrigation system. The aim of the present study was to evaluate various physicochemical parameters (pH, EC, TDS, DO, TA, TH and chlorides) and selected essential/toxic trace metal concentrations (Na, K, Ca, Mg, Sr, Li, Ag, Zn, Fe, Cu, Co, Mn, Cr, Cd, and Pb) in order to explore their distribution, correlation, spatial variations and health risk assessment. Average concentration of some trace metals (Co, Cd and Pb) and physicochemical parameters (EC, TDS, and alkalinity) were found to exceed the national/international standards. Multivariate methods of analysis showed strong associations among Fe–Li–K, Sr–Mg–Ca, Cd–Mn, Cu–Zn, Ag–Co, and Cr–Pb–Na which were significantly contributed by anthropogenic activities. Irrigation water quality index exhibited intermediate suitability of the groundwater for irrigation purpose. Health risk evaluation of the trace metals revealed significant non-carcinogenic risks for Cd, Co and Pb (HQing > 1) especially for children. Similarly, significant carcinogenic risk was found to be associated with Pb and Cr which exceeded the safe limit, suggesting the lifetime carcinogenic risk associated with these metals in the groundwater. The present health risk problems should be considered on top priority and immediate actions should be taken to safeguard the water quality in the study area. [ABSTRACT FROM AUTHOR]

Heavy metal (HM) pollution is one of the major issues of concern in the world due to its serious health consequences on humans and ecology. In this study, riverine water from the River Kabul in Pakistan was studied using inductively coupled plasma mass spectrometry (ICP-MS) to determine the variation, routes, and possible socio-ecological hazards of chromium (Cr), manganese (Mn), cobalt (Co), nickel (Ni), copper (Cu), zinc (Zn) cadmium (Cd), mercury (Hg), and lead (Pb). The results revealed significant HMs variation (p < 0.05) in the sequence of Cr > Zn > Ni > Cu > Cd > Pb > Mn > Co > Hg, indicating prevalent metal contaminations in the river. Multivariate statistics showed significant strong positive correlations (p ≤ 0.01) between the individual HMs contents along the monitoring sites. The strong-moderate levels of Cu, Co, Zn, Mn, Pb, and Cd in riverine systems were observed to be caused by surrounding industrial, agrochemicals, mining, and domestic wastewater discharges along with geogenic sources, the weak levels of Cr and Ni could be induced by erosion of mafic and ultramafic rocks, and mining activities, whereas the low contamination of Hg suggests minimal atmospheric deposition with fewer industrial discharges in the environment. The overall mass flux of the ∑HMs was estimated to be around 164.10 kg/year, with significant HM pollution index (HPI) and pollution index (PI) variations along the river characterizing the potential risk of HMs in decreasing order of Cd > Hg > Cr > Ni > Co > Pb > Mn > Cu > Zn and Cd > Hg > Ni > Pb > Cr > Co > Cu > Mn > Zn, respectively. Individual HM contamination was within the ecological risk threshold (ERI < 110), where, the chronic daily intake (CDIs), hazard quotients (HQs), health indices (HIs), and cancer risks (CRs) of Cd, Ni, Co, Cr, and Pb by daily riverine water ingestion and dermal contact posing considerable human health concerns. To protect the environment and public health, our findings suggest that untreated anthropogenic wastewater discharge into the river system be strictly controlled and regulated through public awareness campaigns and legislation prohibiting the use of herbicides and fertilizers containing high levels of Cr, Ni, Co, Cd, and Pb. [ABSTRACT FROM AUTHOR]

The intake of toxic metals from cooking utensils through food is of growing concern to the medical community. This intake poses serious risk to human health. In many developing countries, different types of contaminated metals scraps are used to make cooking utensils. The leaching of both nutritionally essential and toxic metals in significant quantities from cookware during the cooking process results in food contamination and poses a substantial health risk. In the present study, the leaching of some toxic and potentially toxic metals from cooking utensils into different solutions and food was investigated. A preliminary survey indicated that the majority of individuals tend to use aluminum cookware due to its affordability, overlooking the potential health risks associated with these inexpensive and lower-quality cooking utensils. XRF analysis revealed that aluminum, steel, and copper cookware had K, Ca, Pb, Cd, Ni, V, Sn Mo, Zn, Bi, and Tb as contaminants. In addition, aluminum (3.2 ± 0.25 to 4.64 ± 0.20 g/kg) and copper cookware (2.90 ± 0.12 g/kg) were highly contaminated with lead. The time and pH-dependent study revealed that leaching of metals (Al, Pb, Ni, Cr, Cd, Cu, and Fe, etc.) into food was predominantly from anodized and non-anodized aluminum cookware. More metal leaching was observed from new aluminum cookware compared to old. Acidic food was found to cause more metals to leach during cooking. Blood metal analysis of the local population revealed the presence of high concentrations of Al, Pb, Cd, and Ni. In conclusion, leaching of toxic or potentially toxic metals from cookware into food, especially from anodized and non-anodized aluminum cookware, poses a potential public health risk. Practical applications: Cooking utensils are routinely used for the preparation of food. However, the harmful impact posed by these essential items is largely unknown. The current research briefly explains the toxic metals leaching from cookware in a pH-dependent manner and leaves a message to the public, especially in developing countries like Pakistan, regarding the type of cookware suitable for cooking purposes. [ABSTRACT FROM AUTHOR]

Globally, the hazardous substance in children's goods is of great concern. Toxic chemicals are potentially harmful to the health and growth of infants and children. Lead (Pb) and cadmium (Cd)-contaminated children's jewelry is widely encountered in many countries. This study aims to determine the concentration of metal toxicants (Pb, Cd, Ni, Cu, Zn, Co, and Fe) in event-based (Independence Day festival) children's jewelry, considering time-limited and fast production products that may compromise the quality and safety parameters during manufacturing. The determinations are for the time-constraint industrial production of children's jewelry in the context of the toxic substances in a variety of base materials used. This is the first time event-based children's jewelry has been monitored and critically assessed for metal contamination. Forty-two samples, including metallic, wooden, textile, rubber, plastic, and paint-coated plastic children's jewelry, were tested. Seventy-four percent of samples detected Pb and Cd in quantifiable amounts. Ni in 71%, Cu in 67%, Co in 43%, and Zn and Fe were detected in 100% samples with quantifiable amounts. Twenty-two ID-CJ samples exceeded the US regulatory limit for Pb and four samples for Cd. However, twenty-nine samples for Pb, eleven for Cd, five for Co, and one for Cu exceeded the EU regulatory limit. The highest concentration of Pb was found in paint-coated plastic jewelry, and the highest Cd was found in metallic jewelry. These results suggest that the potential hazards of event-based children's jewelry deserve the attention of government agencies seeking to limit children's exposure to toxic chemicals. Intergovernmental organizations and individual countries regulate chemicals in consumer products, but a coordinated international approach is lacking. Some continents and countries still lack in regulations for children's products, especially jewelry, and toys. [ABSTRACT FROM AUTHOR]

Faisalabad is a major industrial area in Pakistan's Punjab province that discharges wastewater into the Chenab River. Industrial effluents in Faisalabad are predicted to pose a significant threat to the riparian vegetation of the Chenab River and nearby vegetation. Heavy metal pollution of plants, water, and soils is one of the biggest problems worldwide that needs to be addressed because heavy metals above normal levels are extremely dangerous to both riparian vegetation and wildlife. The results indicated high levels of pollution in the industrial effluents as well as in the river in terms of salinity, metal toxicity, TSS, TDS, SAR, the acidic and alkaline nature of the industrial effluents, and the spread of industrial effluents up to 15 square kilometres in the Chenab River. Despite the higher pollution, four plants were found at all sites: Calotropis procera, Phyla nodiflora, Eclipta alba and Ranunculus sceleratus. It was found that most of the selected plants were phytoaccumulators, making them best suited to survive in harsh environments such as those with industrial pollution. The Fe concentration in the plant constituents was the highest, along with Zn, Pb, Cd, and Cu, all of which were above the permissible limits of the WHO. The metal transfer factor (MTF) was higher in most of the plants studied, and even exceeded 10 at some severely affected sites. Calotropis procera proved to be the most suitable plant for growth on drainage systems and also at river sites, as it had the highest importance value across all sites and seasons. [ABSTRACT FROM AUTHOR]

Due to papulation increase in Pakistan the demand of drinking water increased day by day. After the industrial grooming the wastewater producing harmful effect on the surrounding area of industries and drinking water contamination increased. Pakistan is on 82 amongst the 125 countries of the word having drinking water problem. Different parameter set by World Health Organization (WHO) and Pakistan Environmental Protection Agency (Pak‐EPA) of hazard dimension index (HD) and Lifetime Cancerogenic risk Calculation (LFCR) of Potentially toxic metals that are As, Cd, Cr, Ni, Pb (PTMs) are higher, and the school age children are under high risk of cancer. All the drinking constraints set by regulating authority is fully neglected by inopportune human process by solid waste dumping and houses and industrial disposal with any proper treatment. This study discusses all the HD and LFCR parameter in three age group children, male and female through drinking water around the Hattar Industrial Estate (HIE) in Pakistan. In children and women more HD and LFCR thread than the adult man. The overall result shows that the HD risk is higher than the LFCR due to the PTMs in drinking water only HD is exceed from the limit of WHO and Pak‐EPA. If there is no proper arrangement take place for the removal of As and PTMs from the industrial wastewater and drinking water than within few years all the population around the study area effected with different type of cancer. [ABSTRACT FROM AUTHOR]

Heavy metals exposure through dust emissions pose a health risk to workers in coal and chromite mines. The processes involved in mining are noteworthy for the generation of heavy metal-contaminated dust which causes human health implications, especially to the workers that are mainly exposed to such toxins. This study determined pollution levels in coal and chromite mines and calculated the health risk of workers being exposed to heavy metal-contaminated dust. We used fractioned dust with particle sizes < 75, 75–106, and 107–150 µm to assess the pollution levels, anthropogenic impacts, geo-accumulation index, and enrichment factor for selected coal and chromite mines. Through a probabilistic approach, Monte Carlo simulations were used to determine health risks. The findings revealed that the smallest size dust fraction (< 75 μm) contained the highest metal concentrations. Ingestion was considered a prominent exposure route contributing to health risk. In the dust fraction (< 75 μm), chromite mines exhibited the highest Cr (340.6 mg/kg) and lowest Cd (8.4 mg/kg) concentrations. In coal mines, Mn (284.9 mg/kg) and Cd (2.1 mg/kg) were measured highest and lowest, respectively. Pollution assessment revealed dust to be moderately polluted. Health risk assessment showed that Cr in chromite mines exhibited a mean HI value of 1.16E + 00 that was higher than the safe level (HI > 1) having the potential to cause significant health risk to workers. In coal mines, the estimated total HI was 6E-1. Sensitivity analysis revealed concentration and exposure time to be the most influential parameters contributing to risk. Therefore, governmental and nongovernmental organizations must develop dust pollution control guidelines and mitigation measures to safeguard the health of mineworkers by limiting heavy metal exposure. [ABSTRACT FROM AUTHOR]

The present study is based on the measurement of potentially toxic metal contents employing various extraction methodologies aimed at the evaluation of their mobility, bioaccessibility and bioavailability in the urban soil (n = 56) of Lahore, Pakistan. Selected metal levels in the soil were quantified using flame atomic absorption spectrometry. On the average basis, aqua regia and glycine extracts revealed comparatively higher contents for most of the metals; average concentrations of Fe, Mn, Zn, Pb, Cu, Cr, Co and Cd were found at 1566, 451.1, 114.8, 52.84, 39.15, 24.82, 12.59 and 3.953 mg/kg in aqua regia extract, while in glycine extract the metal levels were found at 579.6, 174.2, 74.72, 49.74, 19.28, 7.103, 4.692 and 3.357 mg/kg, respectively. However, Cd, Pb, Cu and Zn showed significantly higher mobility and bioavailability in the soil, while Co, Fe and Mn were least mobile/bioavailable. The pollution index was assessed in terms of enrichment factor and modified degree of contamination which revealed severe to significant contamination and anthropogenic enrichment of Cd, Pb, Cu and Zn. Multivariate analysis showed mostly anthropogenic contributions for Zn–Cu–Cr–Pb–Cd. Health risk assessment revealed relatively higher exposure of the metals through ingestion, while only minor contributions were noted for inhalation and dermal contact. Hazard quotient index was within the safe limit (< 1.0) in all soil extractions, thereby indicating no significant non-carcinogenic health risks. The incremental lifetime cancer risk for Cr (4.1E-06) through ingestion was comparatively higher than the safe limit which showed significant lifetime cancer risk to the local population. [ABSTRACT FROM AUTHOR]

The decontamination of heavy metals contaminated industrial soil is challenging. The use of wild plants is an environment friendly and economical solution. In this study, phytoremediation potential of wild plants collected from the Hayatabad Industrial estate, KPK, Pakistan was evaluated. Total 17 plant species which belongs to 11 family were collected. Plant and soil samples were acid digested and heavy metals including nickel (Ni), copper (Cu), chromium (Cr), cadmium (Cd) and lead (Pb) were analyzed. pH of collected soil ranged between 7.20–8.13, and the dominant soil textures were clay loam, loam and sandy loam. Results showed that among all collected plant species, Erigeron conyzanthus L and Chenopodium murale showed maximum concentration of multi-heavy metals in roots. Collected plant species were not able to accumulate higher concentration of heavy metals in shoots and therefore no plant species was heavy metal hyperaccumulator except Solanum xanthocarpum and Argemone Mexicana accumulated > 1000 mg Pb kg−1 and 300 mg Cr kg−1 in aerial parts, respectively. Solanum xanthocarpum showed maximum metal extraction ratio (%) for all heavy metals. Among all collected plant species, maximum plant species showed translocation factor > 1. Present study showed that collected plant species have ability to transfer heavy metals from roots to shoots and could be used for the phytoremediation of multi-metals contaminated soil. [ABSTRACT FROM AUTHOR]

Purpose: Riverine sediments are an important component of the aquatic environment as they play an essential role in influencing its eco-environmental functions. Sediments may serve as both the source and sink of heavy metals (HM). The Astore River basin is located in the Himalayas, northern Pakistan. The area is frequented by a high number of tourists and is characterized by a fragile biophysical system. This study aimed to assess the occurrence, spatial patterns, and potential sources of HM in the sediments of the Astore River network. Methods: Thirty-nine samples from the Astore River and its tributaries were collected and analyzed for nine HM (Fe, Mn, Cu, Zn, Cd, Cu, Co, Pb, and Ni) using atomic absorption spectrometry. Various modified indices, i.e., modified ecological risk index (mERI) and modified sediments pollution index (mSPI), were utilized to assess the sediment ecological and pollution risks. The data were subjected to compositional data analysis (Coda) using the R program for the source characterization of HM. Results: The results indicated that among the HM, Fe and Cd had the highest and lowest concentrations, respectively. Mn contributed the most to the pollution of the sediments, while Cd has the highest potential ecological impact. The mSPI indicated that the sediments were either in their natural state or showed low to moderate contamination, while only 2.5% were classified as extremely polluted. At the same time, 12.8% of the samples were categorized in the high pollution class. The ecological risk, however, showed more profound and negative bearings on the sediment quality as 33.3% and 2.5% of the samples showed considerable to high ecological risks. The spatial distribution of HM and the modified indices indicated Deosai valley as the zone of concern for management and abatement options. Conclusion: Overall, this study concluded that the major contribution to HM concentration in the area was from natural sources. However, in the case of Cd and Zn, an amplification in concentration due to anthropic factors was noted. Mn and Cd stood out as the major contaminants posing profound ecological risks. A differential spatial pattern of HM contamination in valleys suggested the need for targeted and specific management plans for prevention. [ABSTRACT FROM AUTHOR]

Herein we have determined the heavy metals concentrations (Pb, Cd, and Ni) estimated in River Swat water, irregated soils samples, fodder plant's parts, farmed animal milk/meat samples and human blood from two location Mingora and Barikot in District Swat, Pakistan, using atomic absorption spectrophotometer. Heavy metals such as Cd, Pb, and Ni were found 0.016 ± 0.008, 0.50 ± 0.03, and 0.022 ± 0.004 mg/L in Mingora drain water, respectively, while in the Barikot sample the aforementioned metal were in the range of 0.014 ± 0.01, 0.29 ± 0.02, and 0.108 ± 0.08 mg/L. In the field water samples of Mingora the Cd, Pb and Ni were detected in the range of 0.008 ± 0.001, 0.65 ± 0.05 and 0.032 ± 0.006 mg/L, respectively, while in the field water samples of Barikot contained Cd = 0.016 ± 0.007 mg/L, Pb = 0.48 ± 0.04 mg/L and Ni = 0.043 ± 0.003 mg/L. Drain soil of Mingora contained the highest concentrations of heavy metals, with Cd = 3.9 ± 0.62 mg/kg, Pb = 45.5 ± 3.2 mg/kg, and Ni = 19.95 ± 2.1 mg/kg, whereas field soil contained Cd = 1.6 ± 0.44 mg/kg, Pb = 54.5 ± 2.26 mg/kg, and Ni = 34.75 ± 3.2 mg/kg. Cd in the Barikot drain soil was 3.95 ± 0.71 mg/kg, while it was 0.8 ± 0.06 mg/kg in the field soil. Lead in the same drain sample was 33 ± 3.23 mg/kg, while it was 72 ± 4.15 mg/kg in the field sample. Ni concentrations in Barikot drain soil samples was 13.85 ± 1.1 mg/kg, while 6.9 ± 0.8 mg/kg was detected in field samples. From the results it can be concluded that appreciable concentrations of heavy metals are present in milk and meat samples of farmed animals whereas significant quantities are also there in human blood samples indicating the transport of the selected metals from water through fodder plants into human. However, it may not be the sole reason heavy metal contaminations in human blood other factors like inhalation etc. may also be equally responsible. [ABSTRACT FROM AUTHOR]

Metal pollutants released from motor vehicles are deposited in roadside environments. Metals are non-biodegradable and biomagnify in the food chain causing significant health hazards at all levels of the ecosystem. Hence, management of contaminated roadside verges is critically important and should be kept in mind while planning specific management strategies of such areas. Native vegetation could help to decontaminate heavy metal polluted soils in the best sustainable way. Therefore, this study was designed to assess the potential of Nerium oleander to accumulate heavy metals commonly released by automobiles such as Pb, Cd, Ni, and Zn along with various C and N compounds from five different locations along a busy road in Punjab, Pakistan, during summer and winter seasons. N. oleander showed the ability to absorb C, N, and heavy metals Pb and Cd; the maximum concentration of Pb and Cd was 8.991 mg kg−1 and 0.599 mg kg−1, respectively. These pollutants negatively affected photosynthetic pigments, gas exchange attributes, soluble proteins, and free amino acids. But antioxidant activity of N. oleander was found to be increased in both seasons. The metal accumulation in the plant was higher in the summer though. We highly recommend that by growing N. oleander at roadside verges for decontamination of vehicular pollutants could lead to sustainable management of these corridors. [ABSTRACT FROM AUTHOR]

The amount of heavy metals (HMs) in sediment is regarded as a global crisis, with a large share in developing countries like Pakistan. Water and sediment samples were collected from ten locations of the Kunhar River basin, Pakistan. Eleven HMs such as (As, Cd, Co, Cr, Cu, Fe, Hg, Na, Se, Pb, and Zn) in water and sediments were investigated from Kunhar River basin. According to the results, the Kunhar River's water quality is excellent, except for a slightly higher BOD value that can be minimized by preventing the dumping of human-oriented trash around the river or stream. Concentrations of metals in water have the trend of decreasing in water and sediments, indicating that the accumulation of metals in the sediment can affect the water chemistry of aquatic systems due to any physical or chemical process in the system. River ecosystems already have suffered from impacts of climate change, and these impacts are only expected to worsen. Predictors of climatic variability effects on water quality and quantity may benefit from our research, greater consideration should be given to the variety of metals in relationship to climate variation in order to get a clearer perspective about how much risk aquatic life will be faced by such metals. [ABSTRACT FROM AUTHOR]

This study examined the concentration of heavy metal(loid)s (HM) in groundwater and associated health risks in the Hangu District, Pakistan. Seventy-one groundwater samples were selected from various sources to determine the concentration of twelve HM using the ICP-MS. The average concentrations of HM in groundwater were observed within acceptable guidelines proposed by the World Health Organization (WHO). Similarly, the groundwater of the study area based on the HM contamination index results was noted as an excellent group. Generally, the chronic daily intake (CDI) values for both adults and children were observed in the sequence of Fe > Zn > Ni > Mn > Cu > Sb > Cr > Mo > As > Pb > Co > Cd. The highest hazard quotient was computed for children through groundwater from the tube well. Moreover, both carcinogenic and non-carcinogenic hazards of groundwater were observed in the order of tube well > dug well > spring > bore well. The present study suggests that children were more susceptible to health risks than adults. [ABSTRACT FROM AUTHOR]

Industrialization and urbanization are the major contributors of heavy metal accumulation in soil and vegetables grown under peri-urban agro-ecosystems in Pakistan where farmers usually mix sewage waste water with irrigation water. However, the heavy metal accumulation among different functional groups of insects (i.e. pollinators, pests and predators) largely remains unknown under peri-urban agro-ecosystems. Therefore current study was planned to evaluate the accumulation of copper and nickel heavy metals in insect pollinators, predators and pests on luffa gourd grown in three peri-urban areas of Multan i.e. Soraj Miani, Vehari Chowk and Rangeelpur. Source of irrigation at Soraj Miani was industrial and household sewage water while it was only household sewage water at other two locations. From each of the location, samples of three insect groups (Apis dorsata, Chrysoperla carnea and Spodoptera litura) were collected along with samples of water, plant, and soil. These samples were analyzed using the atomic absorption spectrophotometer (AAS) in order to quantify the accumulation of copper and nickel heavy metals. It was found that residues of copper and nickel metals were present in samples of soil (0.75 Cu, 0.57 Ni µg/g), water (0.10 Cu, 0.14 Ni µg/g), plants (0.13 Cu, 0.19 Ni µg/g) and insect pest (0.15 Cu, 0.15 Ni µg/g) up to varying extent. Therefore, heavy metals can pose serious threat to ecosystem services of pollination and predation in per-urban agro-ecosystem. Future studies should further investigate the impact of heavy metals on other important pollinators and predators in sewage waste water irrigated agro-ecosystems. [ABSTRACT FROM AUTHOR]

Present study was conducted to investigate the content of toxic heavy metals; lead, cadmium, manganese, nickel and zinc in organs of edible fish of River Ravi. Samples of Labeo rohita, Cyprinus carpio, Gibelion catla and Cirrhinus mrigala were captured from two sites at River Ravi i.e. Kala Khatai and Head Balloki. Zinc and lead content was higher in organs of fish specimens collected from Head Balloki than those collected from Village Kala Khatai. Higher average cadmium content was detected in most of the organs of fish collected from Head Balloki than those of Village Kala Khatai. However, higher average manganese content was found in examined organs of fish captured from Village Kala Khatai than those of Head Balloki. Lead and cadmium content in muscles of fish collected from Head Balloki was higher than acceptable level proposed by European Commission (0.2 µg/g and 0.05 µg/g, respectively). Hepatosomatic index of fish species (except L. rohita) collected from Head Balloki was lower than those of Village Kala Khatai. Study has led to the conclusion that edible fish of River Ravi is contaminated with toxic metals and contamination level is higher in fish at Head Balloki as compared to that observed at Village Kala Khatai. [ABSTRACT FROM AUTHOR]

This study examined the physicochemical parameters of water and sediments in the Naltar Lakes, northern Pakistan. Water and sediments were sampled and analyzed for physicochemical parameters. Heavy metals such as iron (Fe, 11% and 12%), nickel (Ni, 100% and 88%), chromium (Cr, 22% and 12%), and arsenic (As, 0% and 12%) of sampling sites had surpassed the threshold of drinking water set by world health organization (WHO) in the Naltar Lake I (Naltar I) and Naltar Lake II (Naltar II), respectively. Water quality parameters were evaluated for the drinking and irrigation water quality indices (WQI). Drinking WQI values were found within the excellent category for Naltar I and Naltar II, except for the 6% of sampling sites observed in the good category. Toxic parameter concentrations were used for the risk indices, which revealed the highest average daily dose (ADD) values of 138 µg/kg-day and hazard quotient (HQ) 1.8 for children through nitrate (NO3) and As consumption in drinking water from the Naltar I and Naltar II, respectively. Water of the Naltar Lakes were characterized by rock weathering dominance. Heavy metal concentrations of sediments showed a moderate level of contamination that poses a low risk to the Naltar Lake ecosystem. [ABSTRACT FROM AUTHOR]

Anthropogenic activities are the major source of chemical pollutants including heavy metals in the coastal ecosystem which may accumulate in the aquatic organisms through the food web. Metal contamination in the marine fish pose serious threat to human health. This study deals with the human health risks of metal accumulation from Sarda orientalis consumption. The quantities of heavy metals were investigated in muscle, gills, and liver of striped bonito from the commercial landings of Karachi Fish Harbor, Pakistan (northern Arabian Sea). Risk for human health was evaluated by iron, zinc, manganese, copper, lead, cadmium, estimated daily intake, hazard index, and risk index. The hazard index value is <1, indicating that the estimated exposure is below reference dose for the relevant metals for monsoon, winter, and pre-monsoon. The mean hazard index for iron, zinc, manganese, copper, lead, and cadmium was 1.20 × 10−2 for consumers. The mean risk index of lead for S. orientalis was 7.39 × 10−9. As a result, the metals did not exceed the World Health Organization's and the United States Environmental Protection Agency's toxicological reference. [ABSTRACT FROM AUTHOR]

Brick kilns constitute one of the major sources of environmental degradation in Pakistan. The present study was conducted to determine the soil quality and total suspended particulates (TSPs) around the brick kilns and assess the health effects on brick kiln workers. Four brick kilns were selected, two each at Sheikhupura and Pattoki city. The soil quality was assessed by analyzing pH, electrical conductivity, moisture content, nitrogen, phosphorus, potassium content, and heavy metal concentration while TSPs were measured by hand-held Microdust Pro Real time monitor. Average TSP concentration around brick kilns at Sheikhupura and Pattoki ranged between 1100 and 1200 µg/m3 and between 550 and 600 µg/m3 respectively which exceeded the Punjab Ambient Air Standards. The soil was slightly acidic (Sheikhupura: average pH = 6.41; Pattoki: average pH = 6.6) while all other analyzed parameters, except heavy metals, were lower than the required FAO Standards for good quality agriculture soil. Heavy metal accumulation in soil samples followed the general order zinc (Zn) > chromium (Cr) > nickel (Ni) > manganese (Mn) > cadmium (Cd) > copper (Cu). For assessment of occupational health and safety issues, all 440 brick kiln workers from the study areas were interviewed. Mostly, brick kiln workers suffered from chronic back pain (84%), skin issues (80.5%), dry cough (72.9%), common cold (46.1%), and shortness of breath (17.1%). The vulnerability risk varied with the nature of the work as workers engaged in brick baking were more vulnerable to respiratory issues than were those in molding task. The study concludes that brick kilns are a source of increased TSP, deteriorated surrounding soil quality, and impaired worker health. It is necessary to regulate these activities and implement requisite health and safety measures. [ABSTRACT FROM AUTHOR]

This study was undertaken in order to determine heavy metal content in fifty-two (n = 52) cosmetic products both imported, locally manufactured and marketed at Quetta, Balochistan. An analytical test was performed for six heavy metal contents in cosmetic products using flame atomic absorption spectrophotometer. The heavy metal which was determined in cosmetic products are Pb, Cr, Mn, Zn, Cu and Fe. The results obtained in this study showed that the concentration of Mn, Zn, Cr and Cu in the analysed samples of each class was relatively higher. It is emphasised that the spurious nature of these products cannot be ignored because developing countries are facing problems to prepare good cosmetics. Hence, some of the vendors are selling locally made products under the brand name of well-reputed national and international companies. Since no safe limit relating to cosmetic products is yet established in Pakistan, therefore, it is difficult to ascertain if the metal concentration values obtained in this study are too high or low. However, literature revealed that prolonged use of such products comprising heavy metals in excess can carry a potential threat to human health and could curb the beauty of the environment. [ABSTRACT FROM AUTHOR]

Land use has a greater influence on the contamination of soils. The current research is conducted to study the effect of different land-use patterns and soil depth on heavy metal fractions in the soil. Significant differences among heavy metals (Cd, Cu, Fe, Ni, Pb, and Zn) in soils under various land use systems were observed. Variations in the soil heavy metals concentration were found dependent on land use patterns. Irrespective of the type of metal, Land uses differed for heavy metals concentrations in the order of fallow>pasture>crop>forest soils. Extracting reagents considerably varied for the concentrations of metals: HNO3>Na2EDTA>NaOH>KNO3>H2O. However, Ni concentration was found higher with NaOH than Na2EDTA. Surface soil indicated a higher magnitude of heavy metals than sub-surface soil. This study will provide valuable information on heavy metal fractions associated with land use in the Abbottabad region of lesser Himalayas, Pakistan. [ABSTRACT FROM AUTHOR]

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Heavy metal pollution has increasingly deteriorated many natural ecosystems around the globe resulting in significant loss of environmental health including endangerment of many biological species. The impact of heavy metal contamination is more pronounced in the developing and resource limited countries due to poor compliance to environmental policies. Pakistan stands on the forefront of pollution threat with unprecedented levels of heavy metal contamination in soils and waters. Phytoremediation has emerged as a promising eco-friendly green technique that has proved to be inexpensive, effective, and easily applicable in different ecosystems in recent years. Different strategies of phytoremediation include phytoextraction, phytovolatilization, phytostabilization, rhizofiltration, and phytofiltration, which have shown promising results against heavy metal-contaminated areas. This paper aims to review the studies conducted in Pakistan in the last two decades that deploy phytoremediation as a green technique to remove heavy metals from the metal-contaminated environment. Merits and demerits as well as advancements and limitations of phytoremediation use in Pakistan are also discussed in the present manuscript. The role of microorganisms in metal uptake is also briefly discussed. The manuscript concludes that despite abundant availability and diversity of plant species, only a few phytoremediation studies have been conducted in Pakistan with limited scope and applicability. Nevertheless, potential of this solar driven green technique is overwhelmingly untapped and during recent years, an increasing trend on phytoremediation studies is evident in the country. [ABSTRACT FROM AUTHOR]

This study was aimed to raise public awareness about the importance of consuming high-quality fish as a healthy protein source for a sustainable lifestyle. Proximate body composition analysis including water, fat, protein, and ash content of farmed and wild caught fish was determined. Fish species i.e. Labeo rohita was selected being one of the most preferred edible fish in the region. The fish samples were collected both from the river and farm using gill nets. To avoid any compromise in fish quality, the fish were kept separately in polythene bags under the laboratory conditions until dispatched for analysis. Statistical analysis using Analysis of Variance (ANOVA) revealed that farmed fish had higher protein content (83.91 %) than wild caught fish (81.42 %). The ash (6.23 %) and moisture (76.12 %) levels in wild fish were higher than in farmed fish (5.59 % and 73.74 %, respectively). Fat content was also higher in wild fish (12.66 %) than in farmed fish (10.53 %). According to the findings, farmed Labeo rohita is of extravagant quality than wild Labeo rohita due to consistent accumulation of heavy metals and pollutants in riverine ecosystems. It is suggested that water and food quality parameters particularly for fish should be under controlled surveillance for health risk assessment and quality assurance. [ABSTRACT FROM AUTHOR]

The current study investigates the water quality parameters of drinking water resources in District Neelam (DNLM), Azad Jammu & Kashmir (AJK), Northwestern Pakistan. The studied area has been recently reported with many waterborne diseases, which probed this analytical study. The samples were aseptically collected from springs, taps, and surface water bodies. The water quality parameters, such as physical, microbiological, anions, and heavy metals, were tested. Results showed that the electrical conductance (EC) and total dissolved solids (TDS), were 974.60 µS/cm and 912.10 mg/L, respectively, exacerbating the quality of drinking water in DNLM. For microbial water testing, we used 3M-Petrifilms as a detection source, which could separate coliform bacteria from E. coli by creating unique surface chromophores. Out of sixty collected samples, 76% had bacterial contamination. Nitrite, nitrate, and phosphate (9.8, 15.0, and 15.1 mg/L), were also surpassing the safe limits of the World Health Organization (WHO) standards for water quality measurement. The heavy metals, i.e., As, Cr, Cu, and Pb were also tested in current analysis. Pb and Cr (0.04 mg/L and 0.06 mg/L) exceeded from safe drinking water guidelines of the WHO and more than 50% of the collected samples had Pb as a major water pollutant in DNLM. Poor waste management, open sludge discharge, lack of municipality measures, and mineral leaching into the freshwaters of DNLM due to mining and metal extraction processes were the main sources of water pollution in the region. The inorganic pollutants were responsible for the sudden rise of different malignancies and other fatal diseases (vital organ failures and reproductive disorders) in the region, which has not been reported in the past. The current investigation yielded useful baseline data of the drinking water reserves of NW Pakistan that could help to develop techniques for the mitigation of water pollutants present in the region. [ABSTRACT FROM AUTHOR]

The study was conducted to analyze three different tissues (liver, gizzard and muscle tissue) of the broiler chicken for absorption of four heavy metal contents (Cu, Zn, Pb and Ni). In addition, analysis of five electrolytes (Ca, Na, K, P and Mg), moisture content, protein, lipids and ash were carried out. The samples were obtained from five different sites of the University Campus, Peshawar-Pakistan. The Atomic Absorption Spectrometry was employed for determination of Cu, Zn, Pb, Ni, Ca and Mg. Furthermore, a spectrophotometer was also used for the purpose of phosphorus (P). However, the analysis of sodium (Na) and potassium (K) content was done through a flame photometer. It was observed that gizzard had the highest concentration of Zn (24.84mg/kg), Pb (2.25mg/kg) and Ni (2.34mg/kg) while, had lowest concentration of Cu (3.30mg/kg) Ca (8.05mg/kg) Na (74.29mg/kg), P (130.67mg/kg) and Mg (15.85mg/kg). Liver had highest concentration of Cu (7.74mg/kg) and Mg (24.23mg/kg) while lowest concentration of Pb (1.24mg/kg) Ni (0.64mg/kg), K (205.20mg/kg) and Na (74.32mg/kg). The muscle tissue come up with highest concentration of Ca, K, Na, P and Mg were 18.65mg/kg, 258.13mg/kg, 110.59mg/kg, 455.53mg/kg and 27.06mg/kg respectively, whereas lowest concentration of Zn (13.13mg/kg). It is concluded that muscle tissue of the broiler chicken is comparatively a good source of macro-nutrients (Protein, Lipid) and micronutrients (Ca, K, Na, P and Mg) and less contaminated with heavy metals. [ABSTRACT FROM AUTHOR]

Water is a vital, finite resource whose quantity and quality are deteriorating as the world population increases. The current study aims to investigate the concentration of heavy metals (HM) in surface water for irrigation purposes with associated human health risks and pollution sources near the marble industry in Malakand, Pakistan. Twenty-seven water samples were randomly collected and analyzed for HM concentration by inductively coupled plasma‒optical emission spectrometry (ICP‒OES). pH, electrical conductivity (EC), total dissolved solids (TDS), biological oxygen demand (BOD), and chemical oxygen demand (COD) were measured using standard methods of American Public Health Association (APHA). Irrigation suitability was assessed using specific water quality parameters. The associated health risks from ingestion and dermal exposure to heavy metals were calculated by USEPA health risk indices. Pollution sources and spatial distribution mapping were studied using compositional data analysis (CoDa) and the application of a geographic information system (GIS) to understand the changing behavior of heavy metals in surface waters. The concentrations of BOD (89%), COD (89%), Al (89%), Ca (89%), Cr (56%), Cu (78%), Fe (56%), K (34%) Mg (23%), Mn (56%), Na (89%), Ni (56%), P (89%), and Zn (11%) exceeded the safety limits of National Environmental Quality standards (NEQs) of Pakistan. The results of Kelly's ratio (KR) classified surface water as unsuitable for irrigation. The average daily doses (ADD, mg/kg/day) for Al, Cu, Cr, Fe, Mn, Ni, and Zn were higher in children than in adults. The hazard index (HI) for children and adults was above the threshold (HI > 1), indicating a significant risk of non-carcinogenic toxicity. The carcinogenic risk values for Cr and Ni were above the USEPA limit (1 × 10−6 to 1 × 10−4), suggesting a potential carcinogenic risk for the target population. Principal component analysis (PCA), biplot (CLR), and the CoDa-dendrogram allowed for the identification of elemental associations, and their potential source was anthropogenic rather than natural in origin. Regular monitoring and phytoremediation strategies are proposed to safeguard crops and human health. [ABSTRACT FROM AUTHOR]