1. Mesoaciditoga lauensis gen. nov., sp. nov., a moderately thermoacidophilic member of the order Thermotogales from a deep-sea hydrothermal vent.
- Author
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Reysenbach AL, Liu Y, Lindgren AR, Wagner ID, Sislak CD, Mets A, and Schouten S
- Subjects
- Bacterial Typing Techniques, Base Composition, DNA, Bacterial genetics, Fatty Acids chemistry, Gram-Negative Anaerobic Straight, Curved, and Helical Rods genetics, Gram-Negative Anaerobic Straight, Curved, and Helical Rods isolation & purification, Heterotrophic Processes, Molecular Sequence Data, Pacific Ocean, Phospholipids chemistry, RNA, Ribosomal, 16S genetics, Gram-Negative Anaerobic Straight, Curved, and Helical Rods classification, Hydrothermal Vents microbiology, Phylogeny, Seawater microbiology
- Abstract
A novel moderately thermophilic, heterotrophic bacterium was isolated from a deep-sea hydrothermal vent deposit from the Mariner field along the Eastern Lau Spreading Center of the south-western Pacific Ocean. Cells were short motile rods (about 0.4×0.8 µm) that occurred singly or in pairs and were surrounded by a sheath-like membrane or 'toga'. The cells grew between 45 and 65 °C (optimum 57-60 °C) and at pH 4.1-6.0 (optimum pH 5.5-5.7) and grew optimally at 3 % (w/v) NaCl. The isolate grew on a range of carbon and proteinaceous substrates and reduced sulfur. The G+C content of the DNA was about 45 mol%. Phylogenetic analysis of the 16S rRNA gene sequence placed the new isolate as a deeply diverging lineage within the order Thermotogales. Based on the physiological, morphological and phylogenetic data, the isolate represents a novel species of a new genus with the proposed name Mesoaciditoga lauensis gen. nov., sp. nov. The type strain of Mesoaciditoga lauensis is cd-1655R(T) ( = DSM 25116(T) = OCM 1212(T)).
- Published
- 2013
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