1. Enhygromyxa salina gen. nov., sp. nov., a slightly halophilic myxobacterium isolated from the coastal areas of Japan.
- Author
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Iizuka T, Jojima Y, Fudou R, Tokura M, Hiraishi A, and Yamanaka S
- Subjects
- Bacterial Typing Techniques, Base Composition, Calcium pharmacology, DNA, Bacterial genetics, Fatty Acids analysis, Japan, Magnesium pharmacology, Molecular Sequence Data, Myxococcales chemistry, Myxococcales classification, Myxococcales drug effects, Myxococcales ultrastructure, Pacific Ocean, Phenotype, Phylogeny, Ribotyping, Seawater microbiology, Sodium Chloride pharmacology, Species Specificity, Vitamin K 2 analysis, Geologic Sediments microbiology, Myxococcales isolation & purification, Water Microbiology
- Abstract
Six isolates of novel marine myxobacteria, designated strains SHK-1T, SMK-1-1, SMK-1-3, SMK-10, SKK-2, and SMP-6, were obtained from various coastal samples (mud, sands and algae) collected around Japan. All of the isolates had Gram-negative rod-shaped cells, motile by gliding and grew aerobically. They showed bacteriolytic action, fruiting body formation, and NaCl requirement for growth with an optimum concentration of 1.0-2.0% (w/v). In addition, divalent cationic components of seawater, such as Mg2+ or Ca2+, were also needed for growth. The major respiratory quinone was MK-7. The G+C content of genomic DNA ranged from 65.6 to 67.4 mol% (by HPLC). The isolates shared almost identical 16S rDNA sequences, and clustered with a recently described marine myxobacterium, Plesiocystis pacifica, as their closest relative on a phylogenetic tree (95.9-96.0% similarity). Physiological and chemotaxonomic differences between the new strains and strains of the genus Plesiocystis justify the proposal of a new genus. Therefore, we propose to classify the six isolates into a new taxon of marine myxobacteria with the name, Enhygromyxa salina gen. nov., sp. nov. The type strain is SHK-1(T) (JCM 11769(T) = DSM 15217(T) = AJ 110011(T)).
- Published
- 2003
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