1. Rapid antigen-capture assay to detect West Nile virus in dead corvids.
- Author
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Lindsay R, Barker Ik, Nayar G, Drebot M, Calvin S, Scammell C, Sachvie C, Fleur TS, Dibernardo A, Andonova M, and Artsob H
- Subjects
- Animals, Manitoba, Ontario, Oropharynx virology, Population Surveillance, Species Specificity, Birds virology, West Nile virus isolation & purification
- Abstract
The utility of the VecTest antigen-capture assay to detect West Nile virus (WNV) in field-collected dead corvids was evaluated in Manitoba and Ontario, Canada, in 2001 and 2002. Swabs were taken from the oropharynx, cloaca, or both of 109 American Crows, 31 Blue Jays, 6 Common Ravens, and 4 Black-billed Magpies from Manitoba, and 255 American Crows and 28 Blue Jays from Ontario. The sensitivity and specificity of the antigen-capture assay were greatest for samples from American Crows; oropharyngeal swabs were more sensitive than cloacal swabs, and interlaboratory variation in the results was minimal. The sensitivity and specificity of the VecTest using oropharyngeal swabs from crows were 83.9% and 93.6%, respectively, for Manitoba samples and 83.3% and 95.8%, respectively, for Ontario birds. The VecTest antigen-capture assay on oropharyngeal secretions from crows is a reliable and rapid diagnostic test that appears suitable for incorporation into a WNV surveillance program.
- Published
- 2003
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