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Plant growth is affected by light availability, light capture, and the efficiency of light energy utilisation within the photosynthetic uptake processes. The radiation use efficiency (RUE) of four even-aged, fully stocked mature Norway spruce stands along a temperature, precipitation, and altitudinal gradient of the Czech Republic was investigated. A new straightforward, methodological approach involving an analysis of digital hemispherical photographs for RUE estimation was applied. The highest annual RUE value (0.72 g MJ -1 ) was observed in the stand characterised by the lowest mean annual air temperature, the highest annual amount of precipitation, located at the highest altitude, and with the lowest site index reflecting site fertility. From the viewpoint of global climate change mitigation, this stand fixed 4.14 Mg ha -1 and 13.93 Mg ha -1 of carbon units and CO 2 molecules into above-ground biomass, respectively. The lowest RUE value (0.21 g MJ -1 ) within the studied growing season was found in the stand located at the lowest altitude representing the site with the highest mean air temperature and the lowest amount of precipitation where 1.27 Mg ha -1 and 4.28 Mg ha -1 of carbon units and CO 2 molecules, respectively, were fixed. From the tested meteorological variables (mean air temperature, the monthly sums of temperature, precipitation, and air humidity), RUE was only significantly dependent on air temperature. Therefore, global warming can lead to diminishing RUE and carbon sequestration in Norway spruce stands, especially at low altitudes.