1. Phylogenetic analysis of urease-positive thermophilic Campylobacter (UPTC) strains based on the molecular characterization of the flaA gene.
- Author
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Murayama M, Tazumi A, Hayashi K, Nakanishi S, Tasaki E, Ueno H, Nakajima T, Matsubara K, Moore JE, Millar BC, and Matsuda M
- Subjects
- Amino Acid Sequence, Animals, Campylobacter classification, Campylobacter isolation & purification, Campylobacter metabolism, Campylobacter lari classification, Campylobacter lari genetics, Campylobacter lari isolation & purification, Campylobacter lari metabolism, Cloning, Molecular, England, Flagellin chemistry, Flagellin metabolism, Molecular Sequence Data, Northern Ireland, Open Reading Frames, Phylogeny, Polymerase Chain Reaction, Recombinant Proteins chemistry, Recombinant Proteins metabolism, Sequence Alignment, Urease metabolism, Bivalvia microbiology, Campylobacter genetics, Flagellin genetics, Recombinant Proteins genetics, Seawater microbiology, Sequence Analysis, DNA methods
- Abstract
Molecular cloning, nucleotide sequencing, and characterization of the flaA gene from additional isolates of urease-positive thermophilic Campylobacter (UPTC) were performed. These isolates were obtained from the natural environment in Northern Ireland (n = 9 from mussels) and in England (n = 1 from sea water). All isolates carried the shorter flaA gene, [open reading frames (ORFs), 1,461 to 1,503 base pairs], without any internal termination codons, and did not carry any flaA pseudogenes. The UPTC isolates were well discriminated by the neighbor joining (NJ) phylogenetic tree constructed based on the putative flaA genes ORFs nucleotide sequence information. In addition, the NJ tree constructed based on the flaA-short variable region sequence information discriminated the Campylobacter lari isolates with a similar degree of discrimination power.
- Published
- 2011
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