1. Characterization of bacteria in ballast water using MALDI-TOF mass spectrometry.
- Author
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Emami K, Askari V, Ullrich M, Mohinudeen K, Anil AC, Khandeparker L, Burgess JG, and Mesbahi E
- Subjects
- Bacteria classification, Bacteria isolation & purification, Bacteriological Techniques methods, DNA, Bacterial chemistry, DNA, Bacterial genetics, Enterococcus genetics, Enterococcus isolation & purification, Molecular Sequence Data, North Sea, Proteus vulgaris genetics, Proteus vulgaris isolation & purification, Pseudoalteromonas genetics, Pseudoalteromonas isolation & purification, Pseudomonas aeruginosa genetics, Pseudomonas aeruginosa isolation & purification, Pseudomonas stutzeri genetics, Pseudomonas stutzeri isolation & purification, Reproducibility of Results, Seawater microbiology, Sequence Analysis, DNA, Vibrio genetics, Vibrio isolation & purification, Bacteria genetics, RNA, Ribosomal, 16S genetics, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization methods, Water Microbiology
- Abstract
To evaluate a rapid and cost-effective method for monitoring bacteria in ballast water, several marine bacterial isolates were characterized by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). Since International Maritime Organization (IMO) regulations are concerned with the unintended transportation of pathogenic bacteria through ballast water, emphasis was placed on detecting species of Vibrio, enterococci and coliforms. Seawater samples collected from the North Sea were incubated in steel ballast tanks and the presence of potentially harmful species of Pseudomonas was also investigated. At the genus-level, the identification of thirty six isolates using MALDI-TOF MS produced similar results to those obtained by 16S rRNA gene sequencing. No pathogenic species were detected either by 16S rRNA gene analysis or by MALDI-TOF MS except for the opportunistically pathogenic bacterium Pseudomonas aeruginosa. In addition, in house software that calculated the correlation coefficient values (CCV) of the mass spectral raw data and their variation was developed and used to allow the rapid and efficient identification of marine bacteria in ballast water for the first time.
- Published
- 2012
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