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The purpose of this research was to collect efficacy data on biological, biochemical, and chemical fungicide seed treatments on hemp (Cannabis sativa L.) to mitigate damping-off and enhance field stand establishment. Seed treatments were evaluated in fields in New York (NY), North Dakota (ND), and Virginia (VA) and at two planting dates in each state in 2020. A single seed lot of a dual-purpose (fiber + grain) cultivar ('Anka') was treated using a laboratory-scale rotary pan coater. Five biological, two biochemical, and four chemical seed treatments were tested. A laboratory germination test revealed that seed treatments did not exhibit phytotoxicity when compared to the non-treated control. A laboratory bioassay with naturally infested soil was used to assess the preliminary activity of seed treatments for protection against damping-off. The biochemical seed treatment Ultim® (active ingredient; organic copper) performed as well as the chemical treatments Apron XL® + Maxim® 4FS and Mertect® 340F in preventing damping-off whereas the biological treatments did not differ from the non-treated control in terms of disease incidence. In all field tests, biological seed treatments did not improve plant stands compared to the non-treated control. Biochemical seed treatments Prudent 44® with Nutrol® (active ingredient; phosphite) and Ultim®, along with chemical seed treatments, had acceptable efficacy and improved stand establishment compared to the non-treated control across field locations. Based on efficacy results from laboratory and field trials, the copper seed treatment has potential for both conventional and organic hemp production. [ABSTRACT FROM AUTHOR]