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Global urbanization has not only promoted social and economic development, but also contributed to seriously ecological challenges. As a type of sustainable landscape patterns, ecological security pattern is considered as an effective spatial pathway to simultaneously conserve ecological security and maintain social-economic development. However, the fragmentation issue of ecological sources of ecological security pattern has not been effectively addressed, although many case studies have been conducted to identify ecological security pattern. In this study, we used spatial conservation prioritization to identify the ecological security pattern of the city belt along the Yellow River in Ningxia, China. Ecological sources were selected using Zonation model while ecological corridors and key ecological nodes were identified with circuit model. The results showed that the ecological security pattern was composed of 97 ecological sources, 226 ecological corridors, 267 pinch points and 22 barriers, covering a total area of 7713.1 km2 and accounting for 34% of the study area. Ecological sources were concentrated in the Helan Mountain, Xiang Mountain and along the Yellow River. Besides, ecological corridors were dense in the southern and eastern part of the study area. Both indicated that the Yellow River and Helan Mountain were the conservation hotspots. Landscape connectivity of ecological sources identified through Zonation-based spatial conservation prioritization was better than that with the scoring approach based on ecosystem service importance. Particularly, in the Zonation approach the landscape connectivity increased with 44% while the average patch area increased with 28% when comparing with the scoring approach. The spatial conservation prioritization approach proposed in this study provides a new effective tool to construct ecological security pattern, which is conducive to the synergic enhancement of landscape connectivity and ecosystem services conservation. • Conservation prioritization was used to identify ecological security pattern. • Ecological sources, corridors, key nodes of Ningxia were identified using Zonation. • The Yellow River and Helan Mountain were ecological conservation hotspots. • Landscape connectivity of ecological security pattern was enhanced. [ABSTRACT FROM AUTHOR]