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Introduction: Accurate and timely information about nicotine and alcohol consumption is needed to inform effective policy. Wastewater‐based epidemiology provides an opportunity to quantify consumption, which can complement traditional data collection methods. Methods: Wastewater samples were collected from seven wastewater treatment plants on seven consecutive days in three regions of New Zealand during the same week as the national census (6 March 2018). Samples were analysed for nicotine and alcohol metabolites using liquid chromatography–tandem mass spectrometry. Detailed catchment maps were developed and per capita consumption calculated. Results: Observed nicotine consumption (mean 1528 ± 412 cigarettes/day/1000 people) was similar to national sales data. Observed alcohol consumption (mean 1155 ± 764 standard drinks/day/1000 people) was lower than estimated using alcohol availability data. Consumption of nicotine and alcohol was generally higher in the Bay of Plenty and Canterbury compared to Auckland, mirroring trends in the New Zealand Health Survey. Intra‐regional differences were observed and the patterns could not be attributed to urbanisation alone. Nicotine consumption was consistent throughout the week whereas alcohol consumption often peaked at the weekend. Nicotine consumption was correlated with neighbourhood‐deprivation. There was little correlation for alcohol. Discussion and Conclusions: Wastewater‐based epidemiology provides a quantitative dataset that complements traditional methods of investigating nicotine and alcohol consumption. Timing data collection to coincide with the census helps to account for the influence of population mobility when normalising consumption. [ABSTRACT FROM AUTHOR]

Introduction and Aims: New Zealand is considered to have unusual drug use patterns by international standards. However, this understanding has largely been obtained from social surveys where respondents self-report use. The aim of this paper is to conduct the first wastewater study of drug use in Auckland.Design and Methods: Wastewater sampling was completed from 2 May to 18 July 2014 at 2 Auckland wastewater treatment plants which service 1.3 million people. Samples were analysed for 17 drug residues by using liquid chromatography-tandem mass spectrometry. Consumption of methamphetamine, 3,4-methylenedioxymethamphetamine (MDMA), cocaine, codeine and methadone (mg/day/1000 people) was estimated by using a back-calculation formula.Results: Methamphetamine, codeine, morphine and methadone were detected with high frequency (80-100%), followed by amphetamine (~60%), MDMA (~7%, i.e. 8 occasions) and methylone (3 occasions). An overall mean of 360 mg of methamphetamine and 60 mg of MDMA was estimated to have been consumed per day per 1000 people. Methamphetamine consumption was found at similar levels in both catchments (377 and 351 mg/day/1000 people). Cocaine was only detected in 1 catchment and on only 8 occasions. JWH-018 was detected in 1 catchment and only on 1 occasion. Methamphetamine, codeine and other opioids were detected at a consistent level throughout the week. 3,4-Methylenedioxymethamphetamine and methylone were detected only during the weekends.Conclusions: Wastewater analysis confirms that methamphetamine was one of the most commonly detected illegal drugs in Auckland and was detected consistently throughout the week. In contrast, cocaine and MDMA were rarely detected, with detection limited to weekends. [Lai FY, Wilkins C, Thai P, Mueller JF. An exploratory wastewater analysis study of drug use in Auckland, New Zealand. Drug Alcohol Rev 2017;00:000-000]. [ABSTRACT FROM AUTHOR]

[Display omitted] • Urinary pesticide metabolites are measured in New Zealand children for the first time. • TCPy and PYR levels are higher than measured in other countries. • TCPy levels are higher for children living on a farm. • Rural residence, dog ownership, and in-home pest control increase pyrethroid exposure. This study aimed to assess pesticide exposure and its determinants in children aged 5–14 years. Urine samples (n = 953) were collected from 501 participating children living in urban areas (participant n = 300), rural areas but not on a farm (n = 76), and living on a farm (n = 125). The majority provided two samples, one in the high and one in the low spraying season. Information on diet, lifestyle, and demographic factors was collected by questionnaire. Urine was analysed for 20 pesticide biomarkers by GC–MS/MS and LC-MS/MS. Nine analytes were detected in > 80% of samples, including six organophosphate insecticide metabolites (DMP, DMTP, DEP, DETP, TCPy, PNP), two pyrethroid insecticide metabolites (3-PBA, trans-DCCA), and one herbicide (2,4-D). The highest concentration was measured for TCPy (median 13 μg/g creatinine), a metabolite of chlorpyrifos and triclopyr, followed by DMP (11 μg/g) and DMTP (3.7 μg/g). Urine metabolite levels were generally similar or low compared to those reported for other countries, while relatively high for TCPy and pyrethroid metabolites. Living on a farm was associated with higher TCPy levels during the high spray season. Living in rural areas, dog ownership and in-home pest control were associated with higher levels of pyrethroid metabolites. Urinary concentrations of several pesticide metabolites were higher during the low spraying season, possibly due to consumption of imported fruits and vegetables. Organic fruit consumption was not associated with lower urine concentrations, but consumption of organic food other than fruit or vegetables was associated with lower concentrations of TCPy in the high spray season. In conclusion, compared to other countries such as the U.S., New Zealand children had relatively high exposures to chlorpyrifos/triclopyr and pyrethroids. Factors associated with exposure included age, season, area of residence, diet, in-home pest control, and pets. [ABSTRACT FROM AUTHOR]

[Display omitted] Controlling importation and transmission of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) from overseas travelers is essential for countries, such as Australia, New Zealand, and other island nations, that have adopted a suppression strategy to manage very low community transmission. Wastewater surveillance of SARS-CoV-2 RNA has emerged as a promising tool employed in public health response in many countries globally. This study aimed to establish whether the surveillance of aircraft wastewater can be used to provide an additional layer of information to augment individual clinical testing. Wastewater from 37 long-haul flights chartered to repatriate Australians was tested for the presence of SARS-CoV-2 RNA. Children 5 years or older on these flights tested negative for coronavirus disease 19 (COVID-19) (deep nasal and oropharyngeal reverse-transcription (RT)-PCR swab) 48 h before departure. All passengers underwent mandatory quarantine for 14-day post arrival in Howard Springs, NT, Australia. Wastewater from 24 (64.9 %) of the 37 flights tested positive for SARS-CoV-2 RNA. During the 14 day mandatory quarantine, clinical testing identified 112 cases of COVID-19. Surveillance for SARS-CoV-2 RNA in repatriation flight wastewater using pooled results from three RT-qPCR assays demonstrated a positive predictive value (PPV) of 87.5 %, a negative predictive value (NPV) of 76.9 % and 83.7% accuracy for COVID-19 cases during the post-arrival 14-day quarantine period. The study successfully demonstrates that the surveillance of wastewater from aircraft for SARS-CoV-2 can provide an additional and effective tool for informing the management of returning overseas travelers and for monitoring the importation of SARS CoV-2 and other clinically significant pathogens. [ABSTRACT FROM AUTHOR]

Glyphosate is the most used herbicide globally, but our understanding of human exposure and how different uses affect exposure is not well understood. The aim of this study was to obtain the first data on glyphosate and its primary degradation product aminomethylphosphonic acid (AMPA) concentrations in pooled and individual urine from the Australia and New Zealand region using a sensitive direct injection method and compare results with studies from elsewhere. Pooled urine samples from the Australian general population (n = 125 pools representing >1875 individuals) and individual urine samples (n = 27) from occupationally exposed New Zealand farmers were analysed by LC-MS/MS. Glyphosate was detected above the LOD (0.20-1.25 μg/L) in 8 % of the Australian population pooled urine samples with most detections in the 45-60 years age group. Furthermore, glyphosate (0.85 to 153 μg/L) and AMPA (0.50 to 3.35 μg/L) were detected in 96 % and 33 % of farmers, respectively. The maximum glyphosate urine concentration was 1.7 times above the recommended acceptable daily intake (ADI), when assuming a urinary excretion rate of 1 %. The pooled sampling and analysis approach proved effective for rapid large-scale screening of populations and could be used to determine where targeted and more specific individual sampling may be required., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier B.V. All rights reserved.)

Aims: A discrete experiment in wastewater-based epidemiology (WBE) timed to coincide with the census was used to investigate the spatial, temporal and socioeconomic patterns of illicit drug consumption in Auckland, Bay of Plenty and Canterbury., Methods: For seven consecutive days over census week (6 March 2018), wastewater was sampled from seven wastewater treatment plants and analysed for methamphetamine, cocaine (as benzoylecgonine) and 3,4-methylenedioxymethamphetamine (MDMA). Detailed sewer catchment maps were developed and, together with the data, were used to analyse drug consumption., Results: Methamphetamine (mean 22.9 ± 9.9 doses/day/1000 people) was the most consumed drug, followed by MDMA (mean 1.7 ± 1.5 doses/day/1000 people) and cocaine (mean 0.5 ± 0.3 doses/day/1000 people). Methamphetamine consumption (and to a lesser extent MDMA) was high compared to that reported for Western nations, while cocaine consumption was extremely low. Cocaine and MDMA consumption were higher in cities compared to towns. In contrast, methamphetamine was typically higher in towns. Cocaine and MDMA were consumed more at weekends. Methamphetamine use was more consistent throughout the week. MDMA and cocaine were correlated with socioeconomic advantage, whereas methamphetamine was correlated with disadvantage., Conclusions: This paper contextualises illicit drug use in three New Zealand regions containing 18.3% of the national population and confirms the pervasiveness of methamphetamine consumption in New Zealand towns. This work demonstrates how WBE can be used to explore the socioeconomic dimensions of drug use when duly combined with other data sources like censuses., Competing Interests: Nil.

In this study, 24-hour composite wastewater samples were collected from a wastewater treatment plant of New Zealand with parallel secondary treatment units. The aim was to investigate the occurrence, removal, and consumption of 13 drugs of abuse (DOAs) including illicit drugs, alcohol, nicotine, and their metabolites. The filtered samples were analysed through direct injection on LC-MS/MS. Ethyl sulfate, one of the major metabolites of alcohol, was detected at the highest concentration (mean = 8300 ng/L) in wastewater influent. The mean concentrations of methamphetamine and hydroxycotinine in the influent were found to be 935 ng/L and 5000 ng/L, respectively. Amphetamine (383 ng/L) and cocaine (286 ng/L) were detected at the highest concentrations in the effluent. The removal efficiency of the treatment plant varied for DOAs: >99% for morphine, ethyl sulfate, and hydroxycotinine and <50% for methadone and 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP). Primary treatment did not show any significant removal of DOAs while the removal efficiencies of total monitored DOAs by Membrane Bioreactor (MBR) and Bardenpho processes were found to be similar (~95% removal). The population was estimated using hydrochemical parameters and human urine biomarkers and showed good agreement with wastewater treatment plant's estimates. Weekday-weekend variation in the consumption of alcohol and methamphetamine was found to be significant, with a higher estimated consumption during the weekends. Monitored DOAs in influent were present at highest concentrations during summer (23 μg/L), at low concentrations during winter (17 μg/L), and at lowest concentrations during heavy rainfall event (11 μg/L), possibly due to dilution. The population normalised mass loads of DOAs were found to correlate with their metabolites, and morphine was found to correlate with nicotine metabolites., (Copyright © 2018 Elsevier B.V. All rights reserved.)

Aims: To compare levels of drug use in Auckland with four Australian major cities using wastewater-based epidemiology (WBE)., Methods: A week of daily wastewater samples were selected from two Auckland and eight Australian urban wastewater treatment plants (WWTPs) during 2014 and 2015. Samples were analysed for drug residues using liquid chromatography-tandem mass spectrometry. Consumption of methamphetamine, methylenedioxymethamphetamine (MDMA), cocaine, codeine and methadone (mg/day/1,000 people) was estimated for each WWTP from mass loads using an internationally validated back-calculation formula., Results: Cocaine was not detected at either of the two Auckland WTTPs, and MDMA was detected on only one day of the sampled week in each of the Auckland WWTPs. In contrast, cocaine and MDMA was detected on every day at all eight Australian WWTPs. Methamphetamine was detected on every day at both the New Zealand and Australian WWTPs. Levels of methamphetamine consumption at the Auckland WWTPs were lower than five of the Australian WWTPs. Lower levels of codeine and methadone consumption were detected in Auckland than Australian sites., Conclusions: MDMA and cocaine use is low in Auckland compared to sampled Australia cities. Both Auckland and the selected Australian cities have significant methamphetamine problems compared to many European cities., Competing Interests: Nil.

Halogenated natural products (HNPs) are frequently detected in marine organisms. High HNP concentrations have previously been found in marine mammals from the Great Barrier Reef, Australia, including in the blubber of herbivorous dugongs (Dugong dugon). To identify the source of HNPs we initially focused on the analysis of Australian seagrass (Halophila ovalis) which serves as the principal food source for dugongs. GC/MS analysis of the seagrass indicated the presence of several organobromine compounds. One compound was identified as 2,3,4,5-tetrabromo-1-methylpyrrole (TBMP) by synthesis. Subsequent analysis of semipermeable membrane devices demonstrated that the photo-sensitive TBMP is widespread in the Great Barrier Reef (Queensland, Australia). The detection of larger TBMP concentrations in fish fillets from Chile and traces in mussels from New Zealand indicated that this potential HNP may be distributed throughout the Southern Pacific Ocean., (Copyright © 2011 Elsevier Ltd. All rights reserved.)