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Hybridisation between related taxa has a range of possible biological consequences, ranging from the production of sterile offspring, through introgression of alleles into populations, to the formation of new species. Examples of plant and animal species hybridising with related taxa abound in the New Zealand region. We review New Zealand examples of hybridisation that have been verified with chromosomal, protein or DNA data. Contemporary hybridisation has been studied at hybrid zones where distinct populations meet and mate in a defined and stable zone of contact. The role of human habitat modification is highlighted with examples of recent range changes that have led to hybridisation and subsequent conservation problems. Hybridisation can result in the swamping of endangered species, although it can also act as a bridge for the transfer of adaptations among lineages. Historical hybridisation in New Zealand has been examined with phylogenetics and there are many examples of organelle introgression or capture. The origin of new species of New Zealand stick insects, ferns and daisies via hybridisation has been demonstrated with cytogenetic and DNA sequence evidence. Thus the importance of hybridisation in the evolution of New Zealand's flora and fauna is highlighted. [ABSTRACT FROM AUTHOR]

Background: Heart failure (HF) is the most common long-term complication of acute myocardial infarction (MI). Understanding plasma proteins associated with post-MI HF and their gene expression may identify new candidates for biomarker and drug target discovery.Methods: We used aptamer-based affinity-capture plasma proteomics to measure 1305 plasma proteins at 1 month post-MI in a New Zealand cohort (CDCS [Coronary Disease Cohort Study]) including 181 patients post-MI who were subsequently hospitalized for HF in comparison with 250 patients post-MI who remained event free over a median follow-up of 4.9 years. We then correlated plasma proteins with left ventricular ejection fraction measured at 4 months post-MI and identified proteins potentially coregulated in post-MI HF using weighted gene co-expression network analysis. A Singapore cohort (IMMACULATE [Improving Outcomes in Myocardial Infarction through Reversal of Cardiac Remodelling]) of 223 patients post-MI, of which 33 patients were hospitalized for HF (median follow-up, 2.0 years), was used for further candidate enrichment of plasma proteins by using Fisher meta-analysis, resampling-based statistical testing, and machine learning. We then cross-referenced differentially expressed proteins with their differentially expressed genes from single-cell transcriptomes of nonmyocyte cardiac cells isolated from a murine MI model, and single-cell and single-nucleus transcriptomes of cardiac myocytes from murine HF models and human patients with HF.Results: In the CDCS cohort, 212 differentially expressed plasma proteins were significantly associated with subsequent HF events. Of these, 96 correlated with left ventricular ejection fraction measured at 4 months post-MI. Weighted gene co-expression network analysis prioritized 63 of the 212 proteins that demonstrated significantly higher correlations among patients who developed post-MI HF in comparison with event-free controls (data set 1). Cross-cohort meta-analysis of the IMMACULATE cohort identified 36 plasma proteins associated with post-MI HF (data set 2), whereas single-cell transcriptomes identified 15 gene-protein candidates (data set 3). The majority of prioritized proteins were of matricellular origin. The 6 most highly enriched proteins that were common to all 3 data sets included well-established biomarkers of post-MI HF: N-terminal B-type natriuretic peptide and troponin T, and newly emergent biomarkers, angiopoietin-2, thrombospondin-2, latent transforming growth factor-β binding protein-4, and follistatin-related protein-3, as well.Conclusions: Large-scale human plasma proteomics, cross-referenced to unbiased cardiac transcriptomics at single-cell resolution, prioritized protein candidates associated with post-MI HF for further mechanistic and clinical validation. [ABSTRACT FROM AUTHOR]

Background: Mental illness is common among the New Zealand population. Several roles have been identified for community pharmacists in providing care to people with mental illness; however, the specifics of these roles have not been studied in New Zealand., Objectives: To explore the services currently provided by community pharmacists, the barriers that may be encountered, and possible solutions when providing care to people with mental illness., Methods: Community pharmacists across the North Island of New Zealand, who expressed a special interest in mental health or had a clozapine-dispensing contract, were invited to participate. Eleven participants were interviewed using a semistructured format guided by principles of inductive analysis. Using a thematic approach, transcripts from the interviews were analyzed with the assistance of NVivo 7® (QSR International Pty Ltd 2007)., Results: Four major themes were identified: provision of care, relationships, challenges within the health care system and resources, and future roles for pharmacists. Provision of care was associated with the type of services pharmacists provided, patient-focused care, family involvement, education, psychological support, referrals, and culturally appropriate practice. Building effective relationships was described by participants as adopting a nonjudgmental and culturally sensitive manner, respecting patient privacy, spending more time with patients, and using varied communication techniques. Significant challenges involved working at the end of the health care chain, inadequate patient information and contact time, prescription and payment issues, and difficulties ensuring compliance with medication. Participants identified education, support, and funding as resources that needed to be improved. Their perspectives about future roles of the pharmacists included providing more education and acting as medicine managers for patients., Conclusions: Pharmacists provided a variety of services to people with mental illness despite the barriers they encountered. If the roles of community pharmacists within mental health were to be expanded, solutions to these barriers would need to be identified and implemented., (Copyright © 2011 Elsevier Inc. All rights reserved.)