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Background: Stream ecosystems comprise complex interactions among biological communities and their physicochemical surroundings, contributing to their overall ecological health. Despite this, many monitoring programs ignore changes in the bacterial communities that are the base of food webs in streams, often focusing on stream physicochemical assessments or macroinvertebrate community diversity instead. We used 16S rRNA gene sequencing to assess bacterial community compositions within 600 New Zealand stream biofilm samples from 204 sites within a 6-week period (February–March 2010). Sites were either dominated by indigenous forests, exotic plantation forests, horticulture, or pastoral grasslands in the upstream catchment. We sought to predict each site's catchment land use and environmental conditions based on the composition of the stream bacterial communities. Results: Random forest modelling allowed us to use bacterial community composition to predict upstream catchment land use with 65% accuracy; urban sites were correctly assigned 90% of the time. Despite the variation inherent when sampling across a ~ 1000-km distance, bacterial community data could correctly differentiate undisturbed sites, grouped by their dominant environmental properties, with 75% accuracy. The positive correlations between actual values and those predicted by the models built using the stream biofilm bacterial data ranged from weak (average log N concentration in the stream water, R2 = 0.02) to strong (annual mean air temperature, R2 = 0.69). Conclusions: Freshwater bacterial community data provide useful insights into land use impacts on stream ecosystems; they may be used as an additional measure to screen stream catchment attributes. [ABSTRACT FROM AUTHOR]

In this paper we test a long-held assumption regarding Otago, New Zealand, goldfields life and death- that scurvy was a ubiquitous and persistent cause of misery and death among the goldminers. We will also explore a parallel argument that the Chinese market gardeners played a large role in stamping out the disease in the goldfields. Through the interrogation of various archival medical primary sources, we show that scurvy was indeed a terrible scourge in the Otago goldfields, but only during the initial rushes into new regions. We also argue that while Chinese market gardeners undoubtedly contributed to a more nutritious and varied diet for European miners and settlers, scurvy had already markedly reduced in frequency by the time of their arrival in the gold fields. Patient-oriented accounts of scurvy in the gold demonstrate the clinical and functional cost of scurvy in during the initial gold rushes of Otago. Furthermore, individual stories of previously anonymous patients found locked in these archival sources demonstrate the importance of re-humanising the past to understand the biological and social context of these frontier times. [ABSTRACT FROM AUTHOR]

Stick insects (Phasmatodea) have many different oviposition strategies, reflecting a range of adaptive behaviours and morphologies to best place and secure eggs in their environments. Oviposition strategies in Aotearoa New Zealand phasmids are not well documented, but the literature so far suggests that they drop individual eggs to the ground from their position in the foliage. Here, we present evidence for an oviposition strategy unique among the Rō stick insects of Aotearoa New Zealand. Individual female Spinotectarchus acornutus were observed inserting their eggs in a range of substrates, in particular the bark of trees and in spaces within the textured surfaces of tree fern trunks. We also highlight how the specific morphology of their eggs may be an adaptation to assist in substrate attachment, while their elongated secondary ovipositor could aid in egg insertion into substrates. [ABSTRACT FROM AUTHOR]

Before European contact, Māori disposed of the dead in environmentally sustainable ways. Revitalizing pre-colonial burial practices presents an opportunity for Māori to evaluate current practices and reconnect with their ancient tribal customs and practices. The research question asks: What is the decolonizing potential of urupā tautaiao (natural burials)? Paradoxically, environmentally unsustainable modern tangihanga (funerals) retain the ethos of customary funerary traditions. Urupā tautaiao presents an opportunity for iwi (tribes) to retain cultural integrity in the death space, without compromising Papatūānuku (earthmother). Methodologically, a Māori worldview frames an action research mindset. The study captures a tribal community's exploratory journey into urupā tautaiao. [ABSTRACT FROM AUTHOR]

Rapid species radiations present difficulties for phylogenetic reconstruction due to lack of phylogenetic information and processes such as deep coalescence/incomplete lineage sorting and hybridization. Phylogenomic data can overcome some of these difficulties. In this study, we use anchored hybrid enrichment (AHE) nuclear phylogenomic data and mitochondrial genomes recovered from AHE bycatch with several concatenated and coalescent approaches to reconstruct the poorly resolved radiation of the New Zealand cicada species in the genera Kikihia Dugdale and Maoricicada Dugdale. Compared with previous studies using only three to five Sanger‐sequenced genes, we find increased resolution across our phylogenies, but several branches remain unresolved due to topological conflict among genes. Some nodes that are strongly supported by traditional support measures like bootstraps and posterior probabilities still show significant gene and site concordance conflict. In addition, we find strong mito‐nuclear discordance; likely the result of interspecific hybridization events in the evolutionary history of Kikihia and Maoricicada. [ABSTRACT FROM AUTHOR]

Background and Objectives: Free, publicly-funded oral health services are available to New Zealand young people up to age 18 years when they transition into private care. We investigate 15-26-year-olds' dental health, care and need in this transition. Methods: The data come from seven cross-sectional, nationally-representative, health surveys run from 2011 to 2018. Each survey sampled from 12,500 to 13,900 people, aged 15+, who were usually resident in New Zealand. Respondents were categorised into four age groups: 15-17, 18-20, 21-23 and 24-26 years. Questions about dental health, care and need were analysed primarily by age group, along with ethnicity and quintiles of the New Zealand Deprivation Index (NZDep). Results: In the older age groups, respondents reported having more teeth removed, less frequent visits to the dentist and were more likely to report unmet need for dental health care with Māori and Pacific peoples and those in the most deprived decile, having less favourable outcomes on these variables. In the youngest age group, there were some differences in access to dental care and unmet need by ethnic group and NZDep quintiles. Conclusion: The dental care of 15-17-year-olds appears to meet the needs of most of this group. Those in younger age groups have better oral health, better access to dental care and have less unmet need than those in the older age groups, with Maori, Pacific people and those in high deprivation groups being most negatively affected. The Government should consider its policy settings to improve the dental health of young adults. [ABSTRACT FROM AUTHOR]

New Zealand alpine environments host a diverse assemblage of insect lineages, with virtually every major insect group represented. The modern mountain ranges of New Zealand are relatively young and large areas of habitat above the tree line have only been in continual existence for the past one million years. We discuss the geological history and physical characteristics of New Zealand alpine environments and the resulting selective pressures placed on insect species. Some notable alpine taxa and previous faunistic research is highlighted. We discuss examples where single lineages have colonised the alpine zone and contrast these with larger radiations of alpine species which in some cases are the result of multiple colonisation events. The age of most alpine lineages is consistent with the young geological age of the mountains, nevertheless there are some much older alpine lineages of uncertain evolutionary history. We show that alpine species have employed a very broad range of morphological, physiological, and behavioural adaptations to survive in the alpine zone, and new studies are starting to unpick their genomic basis. Finally, we look to the future and assess threats to the unique New Zealand alpine insect fauna. [ABSTRACT FROM AUTHOR]

Many mid‐19th‐century immigrants to New Zealand are presumed to have been in pursuit of a "better life" than was achievable in their origin countries. Here, we utilize histological analyses of internal indicators of enamel growth disruption (accentuated lines [ALs]) in 19 European and Chinese immigrants and five colony‐born children from three 19th‐century Otago sites. Observations of regular enamel microstructure were used to estimate a chronology of periods of enamel growth disruption. Clear or potential ALs were present in 18/19 (95%) adults and 4/5 (80%) subadults. Mean occurrence of ALs was higher in Chinese individuals than in European individuals between birth and 1 year of age (Chinese mean = 11; European mean = 3.8) and between 3 and 5 years of age (Chinese mean = 12.7; European mean = 1.3). Potential prenatal ALs were observed in three colony‐born children. Although similar stressors such as malnutrition and infectious disease would have been present in both Europe and China, their expression in these individuals may reflect the embodiment of different push factors that stimulated emigration to New World colonies such as New Zealand. The presence of ALs in colony‐born children indicates some continuation of developmental stressors in New Zealand. The results presented here highlight the value of utilizing microscopic analyses on poorly preserved archeological samples that are frequently excluded from histological examination. This preliminary glimpse into enamel formation disruption challenges the ubiquitous 19th‐century narrative of New Zealand as free from the hardships of industrial revolution era Europe and sheds light on the stresses of childhoods spent in rural China and the potential attractions of the Pacific goldfields in comparison. Future work with larger sample sizes will contribute to a critical exploration of experiences of childhood physiological stress in those who lived and died in colonial New Zealand. [ABSTRACT FROM AUTHOR]

Toxic metal or element exposure has the potential to cause significant negative health effects in human populations. During the goldrushes of the colonial period, mercury amalgamation was one of the most common methods of extracting gold from alluvial deposits or crushed ore, and exposure to mercury was an occupational health hazard. In this study we examine mercury exposure in mining populations from New Zealand's first major goldrush, which began in Central Otago in 1861. We explore mercury toxicity through laser ablation inductively coupled mass spectrometric analysis of archaeological hair and sediment samples associated with the Tuapeka goldfields. Our analysis highlights ubiquitous low‐level mercury exposure on the goldfields, as well as one individual with such high mercury concentrations in their hair that we suspect direct mercury intake, perhaps medicinally, rather than from environmental contamination. [ABSTRACT FROM AUTHOR]

Background: Soil and phyllosphere (leaves and fruit) microbes play critical roles in the productivity and health of crops. However, microbial community dynamics are currently understudied in orchards, with a limited number incorporating temporal monitoring. We used 16S rRNA gene amplicon sequencing to investigate bacterial community temporal dynamics and community assembly processes on the leaves and fruit, and in the soil of 12 kiwifruit orchards across a cropping season in New Zealand. Results: Community composition significantly differed (P < 0.001) among the three sample types. However, the communities in the phyllosphere substrates more closely resembled each other, relative to the communities in the soil. There was more temporal stability in the soil bacterial community composition, relative to the communities residing on the leaves and fruit, and low similarity between the belowground and aboveground communities. Bacteria in the soil were more influenced by deterministic processes, while stochastic processes were more important for community assembly in the phyllosphere. Conclusions: The higher temporal variability and the stochastic nature of the community assembly processes observed in the phyllosphere communities highlights why predicting the responsiveness of phyllosphere communities to environmental change, or the likelihood of pathogen invasion, can be challenging. The relative temporal stability and the influence of deterministic selection on soil microbial communities suggests a greater potential for their prediction and reliable manipulation. [ABSTRACT FROM AUTHOR]

Understanding the musculoskeletal anatomy of soft tissues of the head and neck is important for surgical applications, biomechanical modelling and management of injuries, such as whiplash. Additionally, analysing sex and population differences in cervical anatomy can inform how biological sex and population variation may impact these anatomical applications. Although some muscles of the head and neck are well‐studied, there is limited architectural information that also analyses sex and population variation, for many small cervical soft tissues (muscles and ligaments) and associated entheses (soft tissue attachment sites). Therefore, the aim of this study was to present architectural data (e.g., proximal and distal attachment sites, muscle physiological cross‐sectional area, ligament mass, enthesis area) and analyse sex and population differences in soft tissues and entheses associated with sexually dimorphic landmarks on the cranium (nuchal crest and mastoid process) and clavicle (rhomboid fossa). Through the dissection and three‐dimensional analysis of 20 donated cadavers from New Zealand (five males, five females; mean age 83 ± 8 years; range 67–93 years) and Thailand (five males, five females; 69 ± 13 years; range 44–87 years), the following soft tissues and their associated entheses were analysed: upper trapezius, semispinalis capitis and the nuchal ligament (nuchal crest); sternocleidomastoid, splenius capitis and longissimus capitis (mastoid process); the clavicular head of pectoralis major, subclavius, sternohyoid and the costoclavicular (rhomboid) ligament (rhomboid fossa). Findings indicate that although muscle, ligament and enthesis sizes were generally similar to previously published data, muscle size was smaller for six of the eight muscles in this study, with only the upper trapezius and subclavius demonstrating similar values to previous studies. Proximal and distal attachment sites were largely consistent with the current research. However, some individuals (six of 20) had proximal upper trapezius attachments on the cranium, with most attaching solely to the nuchal ligament, contrasting with existing literature, which often describes attachment to the occipital bone. With respect to sexual dimorphism, the Thai sample exhibited more sex differences in muscle size than the New Zealand sample, but for enthesis size (area), both samples had the same amount of statistically significant sex differences (5 of 10). Additionally, some significant population differences were found when comparing muscle and enthesis size data between the New Zealand and Thai samples. Despite these findings, no sex or population differences were found for ligament size (mass) in either group. This paper presents new architectural data for several understudied areas of the head and neck, as well as providing analyses on sex and population differences, two areas that have limited representation in anatomy. [ABSTRACT FROM AUTHOR]

The Companies Act 1993 provides a process for incorporation of companies and then regulates those companies. 2023 marks thirty years since the inception of our current Act, meaning it has been in place for only half of the life of this journal. Aotearoa New Zealand had general incorporation prior to the current Act. The Companies Act 1993 is significant because we ceased to mirror English legislation; the prior Companies Act 1955 was almost a replica of the Companies Act 1948 (UK). But how independent are we really of our common law legislative forebears? The departures from the English common law may go only as far as drawing features from the North American statutes also based on the common law, even though these departures relate to an earlier version of corporations' law in place before the American Revolution. But is corporate law wholly derived from England and the United States wholly right for New Zealand? This article evaluates the New Zealand company and the application of the Companies Act 1993, asking whether both are fit for purpose in 21st-century Aotearoa New Zealand, a land of small and medium enterprises with a bicultural base and evident desire for more sustainable approaches to enterprise. Questions considered are whether our one-size-fits-all Act, with a key feature extreme ease of incorporation, best serves the needs and aspirations of our peoples and our piece of the planet. And, as a reimagining, what possibilities might be realised with the existing form? [ABSTRACT FROM AUTHOR]

Miscanthus x giganteus (Miscanthus) grass shelterbelts can deliver multiple ecosystem services on New Zealand commercial dairy farms. However, there has been little investigation into how these shelterbelts contribute to insect pest management. Here, on a Canterbury dairy farm, we investigated what generalist surface-dwelling invertebrate natural enemies of insect pests inhabit three separate Miscanthus shelterbelts compared to three unmanipulated field margin plots. The potential contribution of these natural enemy assemblages to future biological control was also investigated. To measure this, live moth egg baits with associated pitfall trapping were deployed in autumn, winter and spring of 2015. Miscanthus and the field margin plots were found to have similar potential natural enemy richness but differed in their community composition. The potential predation rate of pests in Miscanthus was 85% higher than in the field margin. Infrared video and Sanger sequencing confirmed that the harvestman Phalangium opilio and the slug Deroceras reticulatum consumed the egg baits in Miscanthus. Conversely, no bait-consuming invertebrates were identified in the field margin. These preliminary results indicate potential natural enemies inhabit Miscanthus and may consume insect pest eggs on the soil-surface. However, to achieve anyinsect pest suppression, further habitat manipulation would be required. [ABSTRACT FROM AUTHOR]

There is a continued need to consider ways to prevent early adolescent engagement in a variety of harmful risk-taking behaviours for example, violence, road-related risks and alcohol use. The current prospective study examined adolescents' reports of intervening to try and stop friends' engagement in such behaviours among 207 early adolescents (mean age = 13.51 years, 50.1% females). Findings showed that intervening behaviour after three months was predicted by the confidence to intervene which in turn was predicted by student and teacher support although not parental support. The findings suggest that the benefits of positive relationship experiences might extend to the safety of early adolescent friendship groups particularly through the development of confidence to try and stop friends' risky and dangerous behaviours. Findings from the study support the important role of the school in creating a culture of positive adolescent behaviour whereby young people take social responsibility. (Contains 1 figure.)

The use of portfolios in teacher education has grown in popularity over the last decade. Attempts to harness the potential of portfolios as a means to enhance learning and reflection have sometimes led to a complex or document-driven process that appears several steps removed from the act of teaching. In response this paper describes the development of a portfolio process based upon digital photographs taken to document the first teaching practicum of student teachers. Central to students' initial experiences of learning portfolios is a process that is based upon team discussion and reflection, which leads to the successful completion of a group-based portfolio product. (Contains 5 figures.)

The longer-term effectiveness of phonological awareness intervention for children with reading difficulty is likely to be influenced by the quality of general classroom instruction. This descriptive project used a case study design to follow the literacy progress of three children aged six and seven years with speech and language impairment after responding strongly to an intensive phonological awareness intervention. The study analysed the opportunities provided in the children's general classroom programme within the New Zealand English curriculum for ongoing facilitation of phonological awareness skills. Findings indicated that the classroom environment provided multiple opportunities for implicit teaching of phonological awareness skills which may have contributed to the children's ability to maintain skills taught within the intervention. Children with significant reading difficulties underpinned by poor phonological awareness are likely to require ongoing access to intensive intervention in addition to quality classroom instruction.

Importance: In children undergoing heart surgery, nitric oxide administered into the gas flow of the cardiopulmonary bypass oxygenator may reduce postoperative low cardiac output syndrome, leading to improved recovery and shorter duration of respiratory support. It remains uncertain whether nitric oxide administered into the cardiopulmonary bypass oxygenator improves ventilator-free days (days alive and free from mechanical ventilation).Objective: To determine the effect of nitric oxide applied into the cardiopulmonary bypass oxygenator vs standard care on ventilator-free days in children undergoing surgery for congenital heart disease.Design, Setting, and Participants: Double-blind, multicenter, randomized clinical trial in 6 pediatric cardiac surgical centers in Australia, New Zealand, and the Netherlands. A total of 1371 children younger than 2 years undergoing congenital heart surgery were randomized between July 2017 and April 2021, with 28-day follow-up of the last participant completed on May 24, 2021.Interventions: Patients were assigned to receive nitric oxide at 20 ppm delivered into the cardiopulmonary bypass oxygenator (n = 679) or standard care cardiopulmonary bypass without nitric oxide (n = 685).Main Outcomes and Measures: The primary end point was the number of ventilator-free days from commencement of bypass until day 28. There were 4 secondary end points including a composite of low cardiac output syndrome, extracorporeal life support, or death; length of stay in the intensive care unit; length of stay in the hospital; and postoperative troponin levels.Results: Among 1371 patients who were randomized (mean [SD] age, 21.2 [23.5] weeks; 587 girls [42.8%]), 1364 (99.5%) completed the trial. The number of ventilator-free days did not differ significantly between the nitric oxide and standard care groups, with a median of 26.6 days (IQR, 24.4 to 27.4) vs 26.4 days (IQR, 24.0 to 27.2), respectively, for an absolute difference of -0.01 days (95% CI, -0.25 to 0.22; P = .92). A total of 22.5% of the nitric oxide group and 20.9% of the standard care group developed low cardiac output syndrome within 48 hours, needed extracorporeal support within 48 hours, or died by day 28, for an adjusted odds ratio of 1.12 (95% CI, 0.85 to 1.47). Other secondary outcomes were not significantly different between the groups.Conclusions and Relevance: In children younger than 2 years undergoing cardiopulmonary bypass surgery for congenital heart disease, the use of nitric oxide via cardiopulmonary bypass did not significantly affect the number of ventilator-free days. These findings do not support the use of nitric oxide delivered into the cardiopulmonary bypass oxygenator during heart surgery.Trial Registration: anzctr.org.au Identifier: ACTRN12617000821392. [ABSTRACT FROM AUTHOR]

Lettuce necrotic yellows virus is a type of species in the Cytorhabdovirus genus and appears to be endemic to Australia and Aotearoa New Zealand (NZ). The population of lettuce necrotic yellows virus (LNYV) is made up of two subgroups, SI and SII. Previous studies demonstrated that SII appears to be outcompeting SI and suggested that SII may have greater vector transmission efficiency and/or higher replication rate in its host plant or insect vector. Rhabdovirus glycoproteins are important for virus–insect interactions. Here, we present an analysis of LNYV glycoprotein sequences to identify key features and variations that may cause SII to interact with its aphid vector with greater efficiency than SI. Phylogenetic analysis of glycoprotein sequences from NZ isolates confirmed the existence of two subgroups within the NZ LNYV population, while predicted 3D structures revealed the LNYV glycoproteins have domain architectures similar to Vesicular Stomatitis Virus (VSV). Importantly, changing amino acids at positions 244 and 247 of the post-fusion form of the LNYV glycoprotein altered the predicted structure of Domain III, glycosylation at N248 and the overall stability of the protein. These data support the glycoprotein as having a role in the population differences of LNYV observed between Australia and New Zealand. [ABSTRACT FROM AUTHOR]

Experiences of childhood in colonial New Zealand are difficult to reconstruct from the historical record alone. Many of those who came to the colony were illiterate, and the Victorian tendency to avoid discussion of pregnancy and breastfeeding practices restricts our understanding of this important period. Bioarchaeological investigation, however, has the potential to illuminate the life stories of these first-generation Pākehā (European) settlers. Here we use isotopic evidence combined with dental pathology from children interred in a historic cemetery from Otago, New Zealand, to examine colonial childhood. We show how weaning practices in the colony differ from those experienced by their emigrant parents, highlight periods of illness likely associated with the weaning process, and bring to light the potential problems caused by maternal Vitamin D deficiency in the colony. [ABSTRACT FROM AUTHOR]

As people, animals and materials are transported across increasingly large distances in a globalized world, threats to our biosecurity and food security are rising. Aotearoa New Zealand is an island nation with many endemic species, a strong local agricultural industry, and a need to protect these from pest threats, as well as the economy from fraudulent commodities. Mitigation of such threats is much more effective if their origins and pathways for entry are understood. We propose that this may be addressed in Aotearoa using strontium isotope analysis of both pests and products. Bioavailable radiogenic isotopes of strontium are ubiquitous markers of provenance that are increasingly used to trace the origin of animals and plants as well as products, but currently a baseline map across Aotearoa is lacking, preventing use of this technique. Here, we have improved an existing methodology to develop a regional bioavailable strontium isoscape using the best available geospatial datasets for Aotearoa. The isoscape explains 53% of the variation (R2 = 0.53 and RMSE = 0.00098) across the region, for which the primary drivers are the underlying geology, soil pH, and aerosol deposition (dust and sea salt). We tested the potential of this model to determine the origin of cow milk produced across Aotearoa. Predictions for cow milk (n = 33) highlighted all potential origin locations that share similar 87Sr/86Sr values, with the closest predictions averaging 7.05 km away from their true place of origin. These results demonstrate that this bioavailable strontium isoscape is effective for tracing locally produced agricultural products in Aotearoa. Accordingly, it could be used to certify the origin of Aotearoa's products, while also helping to determine if new pest detections were of locally breeding populations or not, or to raise awareness of imported illegal agricultural products. [ABSTRACT FROM AUTHOR]

During the nineteenth century, New Zealand was promoted as a land of plenty, promising a 'better life', to encourage families to settle and develop the growing colony. This paper characterises the life-course of early settlers to New Zealand through historical epidemiological and osteological analyses of the St John's burial ground in Milton, Otago. These people represent some of the first European colonists to Aotearoa, and their children. The analyses provided glimpses into the past of strenuous manual labour, repeated risk of injury, and oral and skeletal infections. Mortality of infants was very high in the skeletal sample and the death certificates outlined the varied risks of infection and accidents they faced. Osteobiographies of seven well-preserved adults demonstrated the detailed narratives that can be gleaned from careful consideration of individuals. The skeletal record indicates childhood stress affecting growth and risk of injury prior to migration. However, the historical record suggests that occupational risks of death to the working class were similar in the new colony as at home. The snapshot of this Victorian-era population provided by these data suggests that the colonial society transported their biosocial landscape upon immigration and little changed for these initial colonists. [ABSTRACT FROM AUTHOR]

Background: Increasing nitric oxide bioavailability may induce physiological effects that enhance endurance exercise performance. This review sought to evaluate the performance effects of consuming foods containing compounds that may promote nitric oxide bioavailability. Methods: Scopus, Web of Science, Ovid Medline, EMBASE and SportDiscus were searched, with included studies assessing endurance performance following consumption of foods containing nitrate, L-arginine, L-citrulline or polyphenols. Random effects meta-analysis was conducted, with subgroup analyses performed based on food sources, sex, fitness, performance test type and supplementation protocol (e.g. duration). Results: One hundred and eighteen studies were included in the meta-analysis, which encompassed 59 polyphenol studies, 56 nitrate studies and three L-citrulline studies. No effect on exercise performance following consumption of foods rich in L-citrulline was identified (SMD=-0.03, p=0.24). Trivial but significant benefits were demonstrated for consumption of nitrate and polyphenol-rich foods (SMD=0.15 and 0.17, respectively, p<0.001), including performance in time-trial, time-to-exhaustion and intermittent-type tests, and following both acute and multiple-day supplementation, but no effect of nitrate or polyphenol consumption was found in females. Among nitrate-rich foods, beneficial effects were seen for beetroot, but not red spinach or Swiss chard and rhubarb. For polyphenol-rich foods, benefits were found for grape, (nitrate-depleted) beetroot, French maritime pine, Montmorency cherry and pomegranate, while no significant effects were evident for New Zealand blackcurrant, cocoa, ginseng, green tea or raisins. Considerable heterogeneity between polyphenol studies may reflect food-specific effects or differences in study designs and subject characteristics. Well-trained males (V̇O2max ≥65 ml.kg.min-1) exhibited small, significant benefits following polyphenol, but not nitrate consumption. Conclusion: Foods rich in polyphenols and nitrate provide trivial benefits for endurance exercise performance, although these effects may be food dependent. Highly trained endurance athletes do not appear to benefit from consuming nitrate-rich foods but may benefit from polyphenol consumption. Further research into food sources, dosage and supplementation duration to optimise the ergogenic response to polyphenol consumption is warranted. Further studies should evaluate whether differential sex-based responses to nitrate and polyphenol consumption are attributable to physiological differences or sample size limitations. Other: The review protocol was registered on the Open Science Framework (https://osf.io/u7nsj) and no funding was provided. [ABSTRACT FROM AUTHOR]

We evaluate a range of blue intensity (BI) tree-ring parameters in eight conifer species (12 sites) from Tasmania and New Zealand for their dendroclimatic potential, and as surrogate wood anatomical proxies. Using a dataset of ca. 10–15 trees per site, we measured earlywood maximum blue intensity (EWB), latewood minimum blue intensity (LWB), and the associated delta blue intensity (DB) parameter for dendrochronological analysis. No resin extraction was performed, impacting low-frequency trends. Therefore, we focused only on the high-frequency signal by detrending all tree-ring and climate data using a 20-year cubic smoothing spline. All BI parameters express low relative variance and weak signal strength compared to ring width. Correlation analysis and principal component regression experiments identified a weak and variable climate response for most ring-width chronologies. However, for most sites, the EWB data, despite weak signal strength, expressed strong coherence with summer temperatures. Significant correlations for LWB were also noted, but the sign of the relationship for most species is opposite to that reported for all conifer species in the Northern Hemisphere. DB results were mixed but performed better for the Tasmanian sites when combined through principal component regression methods than for New Zealand. Using the full multi-species/parameter network, excellent summer temperature calibration was identified for both Tasmania and New Zealand ranging from 52 % to 78 % explained variance for split periods (1901–1950/1951–1995), with equally robust independent validation (coefficient of efficiency = 0.41 to 0.77). Comparison of the Tasmanian BI reconstruction with a quantitative wood anatomical (QWA) reconstruction shows that these parameters record essentially the same strong high-frequency summer temperature signal. Despite these excellent results, a substantial challenge exists with the capture of potential secular-scale climate trends. Although DB, band-pass, and other signal processing methods may help with this issue, substantially more experimentation is needed in conjunction with comparative analysis with ring density and QWA measurements. [ABSTRACT FROM AUTHOR]

Questions: How are plant–plant interactions mediated by environmental factors to influence plant community structure on gravel beaches? Location: Two coastal gravel beaches on the eastern coast of New Zealand. Methods: We surveyed plant co‐occurrence patterns on the beaches and performed plant addition experiments, varying nutrients, water, and presence of potential nurse plants. We used C‐score analysis to test whether observed individual and species co‐occurrence patterns deviated from random expectation. Generalised linear modelling of survival within the experiments tested the importance of potential nurse plants vs both resource‐ and non‐resource‐based stress. Results: Despite the harsh conditions, species co‐occurred less frequently than expected if distributions were random. The presence of adult nurse plants had a negative, rather than facilitative effect on growth and survival of planted shrub seedlings. Abiotic factors also influenced seedling survival, although responses were species‐specific. Conclusions: We found no evidence that facilitation plays a role in structuring plant communities in the highly stressed environment of New Zealand gravel beaches. Rather, our results support the prediction that resource competition is the dominant factor in determining community structure in highly stressed environments and is inconsistent with the stress gradient hypothesis. [ABSTRACT FROM AUTHOR]

Seedlings of New Zealand's treeline-forming Fuscospora cliffortioides (Hook.f.) perform poorly beyond the established canopy, limiting treeline advance. To test the long-standing assumption that photoinhibition impairs regeneration in the subalpine belt of New Zealand's Southern Alps, we assessed photosystem II (PSII) performance of seedling-sized individuals and microclimate variation. We performed diurnal, non-invasive chlorophyll- a -fluorescence measurements on exposed and canopy-sheltered individuals at two sites in New Zealand's Southern Alps during summer and winter. Diurnal recordings of the effective (ΦPSII) and optimal (F v/ F m) photosynthetic quantum yield were supplemented with light response curves and micro-temperature recordings. ΦPSII returned to near-optimal values around 0.8 after 30 min of shading, which rules out accumulative or long-term photoinhibition effects. The maximum electron transport rate derived from rapid light curves was significantly higher (+12%) in exposed compared with canopy-shaded individuals. Summer temperature fluctuated widely on the scree (−0.5 to 60.5 °C) and near seedlings (−2 to 26.5 °C). Our results revealed a remarkable level of light adaptation and contradict previous studies hinting at high light-induced photoinhibition as a treeline-limiting factor in the Southern Alps. By linking low ΦPSII on winter mornings, and large, sudden temperature drops in summer, we suspect that cold-induced photoinhibition might occur but the rapid recovery of ΦPSII seen across a wide temperature range makes lethal photo-oxidative damage rather unlikely. Given the demonstrably low summer frost tolerance of F. cliffortioides , cold-related damage resulting from frost events during the growing season or embolism induced by frost drought may offer more plausible explanations for the poor seedling establishment. Duration and frequency of these events could diminish with global warming, which may promote treeline advance. [ABSTRACT FROM AUTHOR]

We used long read sequencing data generated from Knightia excelsa, a nectar‐producing Proteaceae tree endemic to Aotearoa (New Zealand), to explore how sequencing data type, volume and workflows can impact final assembly accuracy and chromosome reconstruction. Establishing a high‐quality genome for this species has specific cultural importance to Māori and commercial importance to honey producers in Aotearoa. Assemblies were produced by five long read assemblers using data subsampled based on read lengths, two polishing strategies and two Hi‐C mapping methods. Our results from subsampling the data by read length showed that each assembler tested performed differently depending on the coverage and the read length of the data. Subsampling highlighted that input data with longer read lengths but perhaps lower coverage constructed more contiguous, kmers and gene‐complete assemblies than short read length input data with higher coverage. The final genome assembly was constructed into 14 pseudochromosomes using an initial flye long read assembly, a racon/medaka/pilon combined polishing strategy, salsa2 and allhic scaffolding, juicebox curation, and Macadamia linkage map validation. We highlighted the importance of developing assembly workflows based on the volume and read length of sequencing data and established a robust set of quality metrics for generating high‐quality assemblies. Scaffolding analyses highlighted that problems found in the initial assemblies could not be resolved accurately by Hi‐C data and that assembly scaffolding was more successful when the underlying contig assembly was of higher accuracy. These findings provide insight into how quality assessment tools can be implemented throughout genome assembly pipelines to inform the de novo reconstruction of a high‐quality genome assembly for nonmodel organisms. [ABSTRACT FROM AUTHOR]

We evaluate a range of blue intensity (BI) tree-ring parameters in eight conifer species (12 sites) from Tasmania and New Zealand for their dendroclimatic potential, and as surrogate wood anatomical proxies. Using a dataset of ca. 10-15 trees per site, we measured earlywood maximum blue reflectance intensity (EWB), latewood minimum blue reflectance intensity (LWB) and the associated delta blue intensity (DB) parameter for dendrochronological analysis. No resin extraction was performed, impacting low frequency trends. Therefore, we focused only on the high frequency signal by detrending all tree-ring and climate data using a 20-year cubic smoothing spline. All BI parameters express low relative variance and weak signal strength compared to ring-width. Correlation analysis and principal component regression experiments identified a weak and variable climate response for most ring-width chronologies. However, for most sites, the EWB data, despite weak signal strength, expressed strong calibrations with summer temperatures. Significant correlations for LWB were also noted, but the sign of the relationship for most species is opposite to that reported for all conifer species in the Northern Hemisphere. DB performed well for the Tasmanian sites but explained minimal temperature variance in New Zealand. Using the full multi-species/parameter network, excellent summer temperature calibration was identified for both Tasmania and New Zealand ranging from 52 % to 78 % explained variance, with equally robust independent validation (Coefficient of Efficiency = 0.41 to 0.77). Comparison of the Tasmanian BI reconstruction with a wood anatomical reconstruction shows that these parameters record essentially the same strong high frequency summer temperature signal. Despite these excellent results, a substantial challenge exists with the capture of potential secular scale climate trends. Although DB, band-pass and other signal processing methods may help with this issue, substantially more experimentation is needed in conjunction with comparative analysis with ring density and quantitative WA measurements. [ABSTRACT FROM AUTHOR]

Some animal groups, such as stick insects (Phasmatodea), have repeatedly evolved alternative reproductive strategies, including parthenogenesis. Genomic studies have found modification of the genes underlying meiosis exists in some of these animals. Here we examine the evolution of copy number, evolutionary rate, and gene expression in candidate meiotic genes of the New Zealand geographic parthenogenetic stick insect Clitarchus hookeri. We characterized 101 genes from a de novo transcriptome assembly from female and male gonads that have homology with meiotic genes from other arthropods. For each gene we determined copy number, the pattern of gene duplication relative to other arthropod orthologs, and the potential for meiosis-specific expression. There are five genes duplicated in C. hookeri , including one also duplicated in the stick insect Timema cristinae , that are not or are uncommonly duplicated in other arthropods. These included two sister chromatid cohesion associated genes (SA2 and SCC2), a recombination gene (HOP1), an RNA-silencing gene (AGO2) and a cell-cycle regulation gene (WEE1). Interestingly, WEE1 and SA2 are also duplicated in the cyclical parthenogenetic aphid Acyrthosiphon pisum and Daphnia duplex , respectively, indicating possible roles in the evolution of reproductive mode. Three of these genes (SA2 , SCC2 , and WEE1) have one copy displaying gonad-specific expression. All genes, with the exception of WEE1 , have significantly different nonsynonymous/synonymous ratios between the gene duplicates, indicative of a shift in evolutionary constraints following duplication. These results suggest that stick insects may have evolved genes with novel functions in gamete production by gene duplication. [ABSTRACT FROM AUTHOR]

Summary: We evaluated Streptomyces biogeography in soils along a 1200 km latitudinal transect across New Zealand (NZ). Streptomyces diversity was examined using high‐throughput sequencing of rpoB amplicons generated with a Streptomyces specific primer set. We detected 1287 Streptomyces rpoB operational taxonomic units (OTUs) with 159 ± 92 (average ± SD) rpoB OTUs per site. Only 12% (n = 149) of these OTUs matched rpoB sequences from cultured specimens (99% nucleotide identity cutoff). Streptomyces phylogenetic diversity (Faith's PD) was correlated with soil pH, mean annual temperature and plant community richness (Spearman's r: 0.77, 0.64 and −0.79, respectively; P < 0.05), but not with latitude. In addition, soil pH and plant community richness both explained significant variation in Streptomyces beta diversity. Streptomyces communities exhibited both high dissimilarity and strong dominance of one or a few species at each site. Taken together, these results suggest that dispersal limitation due to competitive interactions limits the colonization success of spores that relocate to new sites. Cultivated Streptomyces isolates represent a major source of clinically useful antibiotics, but only a small fraction of extant diversity within the genus have been identified and most species of Streptomyces have yet to be described. [ABSTRACT FROM AUTHOR]

Relying solely on public conservation lands for habitat provision will be inadequate for achieving national conservation goals. Production landscapes in New Zealand make up 60% of the land area and contain potential conservation habitat; however, the amount of native vegetation they contain is poorly known. While there have been previous assessments of native vegetation cover in New Zealand, no study has undertaken a national-scale assessment of multiple native vegetation cover types on different land uses. This absence limits the potential to manage production landscapes for conservation. Our study aimed to bridge this gap by using GIS and remote-sensing data to estimate the area of native vegetation, including forests, grasslands and wetlands, present on different land-use classes and in different environments. We found that while most of the country's remaining native vegetation was found on public conservation land, it was not evenly distributed across land environments and was biased towards high-elevation vegetation types. Yet private land, in particular sheep and beef farms, contained a quarter of the remaining native vegetation in the country, and 17% of remaining native forest. While this vegetation was often highly fragmented, it contained forest types that were otherwise under-represented on public conservation land. We conclude that sheep and beef farms in New Zealand have the potential to add to nationwide conservation efforts. However, realising this potential will involve improving the connectivity, area and quality of native vegetation. [ABSTRACT FROM AUTHOR]

Despite one-third of New Zealand's landmass being protected as public conservation land, the country still faces significant conservation challenges. Nearly 50% of the country's landmass has been converted to pastoral farming, and biological invasions pose a sustained and growing threat to remaining biodiversity across all land tenures. Managing and protecting biodiversity on-farm provides vast opportunities to create nature-rich pastoral landscapes. A key step towards bringing about necessary behaviour change to achieve this is increasing insights into farmers' attitudes towards the value of native biodiversity on their farms. Using a questionnaire underpinned by the Theory of Planned Behaviour, we surveyed 500 sheep and beef farmers from around New Zealand as to their beliefs and attitudes and perceived barriers relating to the protection and management of native biodiversity on their farm. Although the survey respondents were largely homogenous, the surveyed group of farmers were heterogeneous in their responses. When asked about advantages associated with managing and protecting native biodiversity on their farms, 690 distinct responses were provided, spanning social (47%), environmental (34%), practical (10%), and economic (2%) themes. In contrast, identified disadvantages were fewer (530 distinct responses) and less wide-ranging in scope, clustering around economic (44%), practical (26%) and social (25%) themes. Nearly three times as many farmers stated there were no disadvantages (22%) than stated there were no advantages (8%). However, the most frequently cited disadvantages were cost and time, which were also commonly cited as barriers to managing biodiversity. Our study illustrates that sheep and beef farmers perceive may advantages in maintaining native biodiversity on-farm, but there is a clear desire for greater support in overcoming identified barriers and this will require a targeted policy response. [ABSTRACT FROM AUTHOR]

Spider webs vary in size to meet the nutritional requirements of the resident spider with the resident's body size strongly informing these requirements. In this way, the effect of body size on web-building behaviour should be apparent across species. To determine whether the size of analogous web structures scales with body size across closely related species, we first measured mainsheet area and adult female body size of 12 sheet-web spider species (Cambridgea). Using these species, we then generated alignments from the cytochrome c oxidase subunit I (COI) and histone 3 (H3) gene regions. These alignments were phylogenetically analysed using Bayesian inference and maximum likelihood methods. While phylogenetic trees for the COI gene suggested that Cambridgea is monophyletic relative to sampled outgroups, H3 did not. Combining our COI phylogenetic tree's branch lengths with data on web-building behaviour, we used phylogenetic least squares to determine whether web size scales with spider size across species. While we found evidence that larger species generally build larger webs, the variation in web size across even similarly sized species suggests that environmental characteristics which influence site selection and prey type may play a role in determining the optimal web size for different species. [ABSTRACT FROM AUTHOR]

Aim: During the late Oligocene (23 mya) the New Zealand landmass was reduced to approximately 18% of its current area. It has been hypothesized that this event, known as the Oligocene Drowning, caused population bottlenecking and mass extinction. Using phylogenetic methods, we examine the effect of this and other environmental events on the hyper‐diverse Zopheridae beetles (162 morphospecies), which largely inhabit leaf litter and dead wood. Location: New Zealand. Taxon: Zopheridae, Coleoptera. Methods: Here we use a fossil‐calibrated phylogenetic tree estimated from mitochondrial cytochrome c oxidase subunit I and nuclear large subunit rRNA genes to identify monophyletic New Zealand zopherid lineages and date the age of these lineages. We used Bayesian diversification models (compound Poisson process on mass extinction times) to test the hypothesis that the New Zealand zopherids underwent a mass extinction in the late Oligocene followed by an increase in speciation rate in the Miocene. We also used these data to estimate the age of these lineages in New Zealand. Results: We demonstrate that 15–20 zopherid lineages survived the Oligocene Drowning depending on the calibration scheme. Of these lineages from 3 to 11 have posterior intervals that encompass the rifting of New Zealand from Gondwana in the late Cretaceous, again depending on the calibration scheme. The diversification model shows no evidence of an increase in extinction rate during the Oligocene Drowning or during any other period since the Cretaceous. Furthermore, rather than recovering an increase in speciation rate during the Miocene and Pliocene, due to environmental changes, we instead recovered a large drop in the speciation rate during this time. Main conclusions: The New Zealand zopherid fauna is a combination of lineages, some of which may have existed on New Zealand since the rifting from Gondwana and other more recent arrivals. The late Oligocene reduction in land area was insufficient to cause a mass extinction in the Zopheridae. This suggests the amount of emergent land was great enough to support a diverse invertebrate fauna. Our study demonstrates the different biogeographic patterns evident in cryptic, hyper‐diverse, and poorly dispersing invertebrate species relative to more mobile plants and animals. [ABSTRACT FROM AUTHOR]

Inhibitory control is central to developmental trajectories of cognitive, emotional and social functioning in children. Here, inhibitory control was measured using the Luria hand clap task (an adapted version of the Luria pencil tap task). A sample of 5,448 children age 4½ years and their mothers from a longitudinal, population-based New Zealand cohort were included in this study. Antenatal maternal health indictors, child characteristics and performance on the Luria hand clap task were assessed using multivariate ordinal logistic regression. Seven covariates, including mother's ethnicity and education, and child's gender, age and birthweight were included. Based on Luria task performance scores that approximated the 25th and 50th percentiles, children were categorised into three groups (low, intermediate, high). High Luria task performance was associated with maternal relationship status, maternal education, first trimester folate supplementation, maternal BMI and smoking before pregnancy. In addition, children born heavier, and female, and older children were more likely to be in the top Luria score category relative to children born smaller, male and younger. A number of potentially modifiable maternal factors significantly predict Luria task performance in children age 4½ years. Identifying socio-demographic, child characteristics and maternal factors that are associated with inhibitory control is a crucial first step for identifying children at risk of atypical inhibition development and informing behavioural intervention strategies. [ABSTRACT FROM AUTHOR]

Pastoral farming is the dominant land use in New Zealand today and is under considerable domestic social and political pressure to reduce its environmental footprint. In this article, we explore options to enhance native biodiversity conservation within New Zealand pastoral systems. We argue that there is strong synergistic interdependence between biodiversity conservation and pastoral farming and suggest that it is possible to have win-win outcomes for both. Landowners need to be incentivised and rewarded for good biodiversity management, rather than relying on a strict rules-based approach. To bring integrity and objective support to this incentive-based approach, farmers need to adopt environmental management planning that is supported by good biodiversity extension resources. Alongside this, a verification system is required that shows farmers are doing what they say they are doing and reflects agreed management targets for biodiversity. This approach requires trust and partnership among all players in agroecosystems - farmers, government, food and fibre processors, scientists, conservationists, NGOs, and the wider New Zealand population. We suggest that if we change the way we think about how farming and biodiversity interact, then we will achieve substantial biodiversity gains across the 50% of New Zealand under pastoral farming. This then brings integrity to the existing and expanding market story for pastoral farming and creates a stronger connection between all New Zealanders and the farming sector. Advancing our thinking in this way will enable New Zealand to maintain a premium for our farming products internationally while supporting conservation of our native biodiversity. [ABSTRACT FROM AUTHOR]

This account presents information on all aspects of the biology of Crassula helmsii (Kirk) Cockayne (New Zealand Pygmyweed, Australian Swamp Stonecrop) that are relevant to understanding its ecological characteristics and behaviour. The main topics are presented within the standard framework of the Biological Flora of the British Isles: distribution, habitat, communities, responses to biotic factors, responses to environment, structure and physiology, phenology, floral and seed characters, herbivores and disease, history, and conservation.Crassula helmsii is an invasive aquatic macrophyte originating from Australia and New Zealand. The first naturalized population was recorded in Britain in 1956. All British specimens appear to represent a single lineage. DNA analysis indicates that British specimens originate from Australia.Crassula helmsii is capable of growing in a wide range of lentic freshwater and riparian habitats. It may grow as free floating, submerged, emergent or terrestrial forms. Morphology can differ markedly on the same stem.Flowers are produced, but with no known pollinators outside of its native range. Reproduction is thought to be predominantly by asexual methods outside Australia and New Zealand, through stem fragmentation and dispersal. Seed germination outside the native range appears to be very rare and poorly understood.Crassula helmsii has been and still is controlled by active management because of fears that its capacity to produce monocultures will lead to loss of biodiversity in the plant communities it invades. Evidence for species losses due to colonization by C. helmsii is rather anecdotal, although suppression of native biomass through competition has been reported. No consistently effective control procedures have been identified. [ABSTRACT FROM AUTHOR]

Flammability is an important plant trait, relevant to plant function, wildfire behaviour and plant evolution. However, systematic comparison of plant flammability across ecosystems has proved difficult because of varying methodologies and assessment of different fuels comprising different plant parts. We compared the flammability of plant species at the leaf‐level (most commonly used in flammability studies) and shoot‐level (which retains aspects of plant architecture). Furthermore, we examined relationships between leaf functional traits and flammability to identify key leaf traits determining shoot‐level flammability.We collated and analysed existing leaf‐ and shoot‐level flammability data from 43 common indigenous perennial New Zealand plant species, along with existing data on leaf morphological and chemical traits.Shoot‐level flammability was decoupled from leaf‐level flammability. Moreover, leaf‐level rankings of flammability were not correlated with rankings of flammability of plants derived from expert opinion based on field observations, while shoot‐level rankings had a significant positive relationship. Shoot‐level flammability was positively correlated with leaf dry matter content (LDMC), phenolics and lignin, and negatively correlated with leaf thickness.Synthesis. Our study suggests that shoot‐level measurements of flammability are a useful and easily replicable way of characterizing the flammability of plants, particularly canopy flammability. With many parts of the world becoming more fire‐prone, due to anthropogenic activities, such as land‐use change and global warming, this finding will help forest and fire managers to make informed decisions about fuel management, and improve modelling of fire‐vegetation‐climate feedbacks under global climate change. Additionally, we identified some key, widely measured leaf traits, such as leaf dry matter content (LDMC), that may be useful surrogates for plant flammability in global dynamic vegetation models. [ABSTRACT FROM AUTHOR]

Background: Falls are two to four times more frequent amongst older adults living in long-term care (LTC) than community-dwelling older adults and have deleterious consequences. It is hypothesised that a progressive exercise program targeting balance and strength will reduce fall rates when compared to a seated exercise program and do so cost effectively.Methods/design: This is a single blind, parallel-group, randomised controlled trial with blinded assessment of outcome and intention-to-treat analysis. LTC residents (age ≥ 65 years) will be recruited from LTC facilities in New Zealand. Participants (n = 528 total, with a 1:1 allocation ratio) will be randomly assigned to either a novel exercise program (Staying UpRight), comprising strength and balance exercises designed specifically for LTC and acceptable to people with dementia (intervention group), or a seated exercise program (control group). The intervention and control group classes will be delivered for 1 h twice weekly over 1 year. The primary outcome is rate of falls (per 1000 person years) within the intervention period. Secondary outcomes will be risk of falling (the proportion of fallers per group), fall rate relative to activity exposure, hospitalisation for fall-related injury, change in gait variability, volume and patterns of ambulatory activity and change in physical performance assessed at baseline and after 6 and 12 months. Cost-effectiveness will be examined using intervention and health service costs. The trial commenced recruitment on 30 November 2018.Discussion: This study evaluates the efficacy and cost-effectiveness of a progressive strength and balance exercise program for aged care residents to reduce falls. The outcomes will aid development of evidenced-based exercise programmes for this vulnerable population.Trial Registration: Australian New Zealand Clinical Trials Registry ACTRN12618001827224. Registered on 9 November 2018. Universal trial number U1111-1217-7148. [ABSTRACT FROM AUTHOR]

New Zealand's formerly extensive lowland native forests have been comprehensively cleared or modified, and large areas of secondary-growth vegetation have subsequently established. These areas are comprised of native, exotic, and mixed tree and shrub species assemblages. The mature-phase canopy and emergent tree species representative of pre-human New Zealand forests are often rare or locally extinct in these forests, indicating negative ramifications for long-term biodiversity conservation and ecosystem service provision, especially such as carbon sequestration. The successful recruitment of mature-phase canopy and emergent tree species may be prevented by biotic and abiotic filters related to dispersal (e.g. lack of seed sources or lack of dispersal agents), environmental variation (e.g. unsuitable germination microclimate or light availability), and competition (e.g. exotic weed competition). Failure of mature-phase tree species to cross through these filters may halt forest succession and cause arrested development of the ecosystem. There are also social and cultural imperatives for restoring mature-phase tree species, such as reassembling desired forest habitat and landscapes and providing lost natural heritage and cultural resources. Therefore, to restore secondary forests, depauperate remnant forests and create new forests that have complex structure, high biomass, and natural canopy tree diversity, mature-phase canopy and emergent species should be reintroduced through human interventions (i.e. enrichment planting). Experiments demonstrate that mature-phase tree species establishment can be optimised through canopy manipulation to address competition for light. Such targeted management can determine successful recruitment of mature-phase tree species, as can weed maintenance post-enrichment planting and landscape-level pest animal control. Currently political focus is emphasising planting of new early-successional native forests. However, support from scientific research and policy development is essential to actively recruit mature-phase tree species where they are now poorly represented and hence forest succession may be arrested. Afforestation and emissions trading policies need to support the reinstatement of mature-phase tree species within existing regenerating and degraded forests and newly created forests to facilitate the substantial ecological and ecosystem service benefits they provide over the long-term. [ABSTRACT FROM AUTHOR]

Objective: To test whether a telephone follow-up service for high-needs ethnic groups increases bowel screening participation in non-responders to postal invitations. Methods: Māori, Pacific, and Asian ethnicity individuals who failed to return a bowel screening test kit within four weeks of it being posted were randomly allocated (1:1) to a telephone active follow-up service or a control group. The active follow-up service made multiple attempts to contact the invitee and provide support. Participation rates at eight weeks' post-randomisation were compared, and the effect of the intervention on overall participation rates was imputed. Results: A total of 3828 eligible individuals were allocated to active follow-up and 3773 to the control group. The imputed potential overall increase in participation in the active follow-up group was 2.0% (95% CI = 0.6%–3.4%); however, the impact of follow-up varied significantly by ethnicity and deprivation. The imputed increase in participation was significant for Māori (5.2; CI = 1.8%–8.5%) and Pacific (3.6%; CI = 0.7%–6.4%), but not for Asian ethnicities (0.7%; CI=−1.1%–2.4%). In addition, the imputed increase was significant among high deprivation participants (3.9%; CI = 2.0%–5.9%), but not among low deprivation participants (0.3%; CI=−1.6%–2.2%). Conclusions: Active follow-up led to higher bowel screening participation in Māori and Pacific but not in Asian ethnicities and was more effective in high deprivation subjects. Active follow-up significantly reduced but did not eliminate ethnic inequalities in bowel screening participation. [ABSTRACT FROM AUTHOR]

Global biodiversity loss is accelerating at an alarming rate. While considerable effort and resources have gone into conservation management for many threatened species in New Zealand (NZ), some species are still 'losing the battle' despite much effort, and others have been ignored altogether. Here, we present seven case studies to illustrate the breadth of complex, often ambiguous, threats faced by taxa in NZ. These threats originate from the effects of agriculture and harvesting, irreversible habitat modification and loss, impediments to connectivity, disruption of parasite–host relationships, introduced species and susceptibility to disease, and are further exacerbated by complexities of political and legal inertia, low prioritisation and limited conservation funding. We outline the conservation challenges and identify advances needed to meet NZ's long-term conservation goals. The next 30 years of conservation require new tools in order to protect especially those 'intractable' species that have thus far defied efforts to ensure their survival. [ABSTRACT FROM AUTHOR]

Sabulopteryx botanica Hoare & Patrick, sp. nov. (Lepidoptera, Gracillariidae, Gracillariinae) is described as a new species from New Zealand. It is regarded as endemic, and represents the first record of its genus from the southern hemisphere. Though diverging in some morphological features from previously described species, it is placed in genus Sabulopteryx Triberti, based on wing venation, abdominal characters, male and female genitalia and hostplant choice; this placement is supported by phylogenetic analysis based on the COI mitochondrial gene. The life history is described: the larva is an underside leaf-miner on the endemic divaricating shrub Teucrium parvifolium (Lamiaceae), and exits the mine to pupate in a cocoon in a folded leaf of the host plant. The remarkable history of the discovery and rediscovery of this moth is discussed: for many years it was only known from a single sap-feeding larva found in a leafmine in a pressed herbarium specimen of the host. The adult was discovered by BHP in Christchurch Botanic Gardens in 2013. Most distribution records of the moth come from a recent search for mines and cocoons on herbarium specimens of T. parvifolium. Sabulopteryx botanica has high conservation status, and is regarded as 'Nationally Vulnerable' according to the New Zealand Department of Conservation threat classification system, based on the rarity and declining status of its host plant. However, the presence of apparently thriving populations of S. botanica on cultivated plants of T. parvifolium, especially at the type locality, Christchurch Botanic Gardens, suggests that encouraging cultivation of the plant could greatly improve the conservation status of the moth. A revised checklist of New Zealand Gracillariidae is presented, assigning all species to the currently recognised subfamilies. The Australian Macarostola ida (Meyrick, 1880) is newly recorded from New Zealand (Auckland), where it is established on Eucalyptus. [ABSTRACT FROM AUTHOR]

Documenting natural hybrid systems builds our understanding of mate choice, reproductive isolation and speciation. The stick insect species Clitarchus hookeri and C. tepaki differ in their genital morphology and hybridize along a narrow peninsula in northern New Zealand. We utilize three lines of evidence to understand the role of premating isolation and species boundaries: (a) genetic differentiation using microsatellites and mitochondrial DNA; (b) variation in 3D surface topology of male claspers and 2D morphometrics of female opercular organs; and (c) behavioural reproductive isolation among parental and hybrid populations through mating crosses. The genetic data show introgression between the parental species and formation of a genetically variable hybrid swarm. Similarly, the male and female morphometric data show genital divergence between the parental species as well as increased variation within the hybrid populations. This genital divergence has not resulted in reproductive isolation between species, instead weak perimating isolation has enabled the formation of a hybrid swarm. Behavioural analysis demonstrates that the entire mating process influences the degree of reproductive isolation between species undergoing secondary contact. Mechanical isolation may appear strong, whereas perimating isolation is weak. [ABSTRACT FROM AUTHOR]

Exposure to low temperatures requires an organism to overcome physiological challenges. New Zealand wētā belonging to the genera Hemideina and Deinacrida are found across a wide range of thermal environments and therefore subject to varying selective pressures. Here we assess the selection pressures across the wētā phylogeny, with a particular emphasis on identifying genes under positive or diversifying selection. We used RNA-seq to generate transcriptomes for all 18 Deinacrida and Hemideina species. A total of 755 orthologous genes were identified using a bidirectional best-hit approach, with the resulting gene set encompassing a diverse range of functional classes. Analysis of ortholog ratios of synonymous to nonsynonymous amino acid changes found 83 genes that are under positive selection for at least one codon. A wide variety of Gene Ontology terms, enzymes, and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathways are represented among these genes. In particular, enzymes involved in oxidative phosphorylation, melanin synthesis, and free-radical scavenging are represented, consistent with physiological and metabolic changes that are associated with adaptation to alpine environments. Structural alignment of the transcripts with the most codons under positive selection revealed that the majority of sites are surface residues, and therefore have the potential to influence the thermostability of the enzyme, with the exception of prophenoloxidase where two residues near the active site are under selection. These proteins provide interesting candidates for further analysis of protein evolution. [ABSTRACT FROM AUTHOR]

Genetic technologies such as gene editing and gene drive systems have recently emerged as potential tools for pest control. Gene drives, in particular, have been described as potential solutions to the pest problems that beset New Zealand. Here we describe the current state of gene drive technologies and present a series of examples to examine the potential benefits and problems arising from gene drive approaches for pest control in New Zealand. We consider the risks and barriers, both biological and social, that would need to be addressed to deploy such systems against our key pests with particular reference to the unique characteristics of New Zealand’s biota, environment and peoples. Gene drives are a potentially useful technology for the eradication of pests in New Zealand but a great deal of research and understanding, as well as public acceptance, is required before they can be implemented. [ABSTRACT FROM AUTHOR]

The nineteenth century New Zealand goldfields were a place where people from across the world came together in search of their fortunes. Written accounts of life on the diggings do exist but are of varying veracity and we therefore have little knowledge of the life experiences of those who came seeking gold. Recent excavations in cemeteries associated with the Otago goldrushes, however, are allowing direct reconstruction of lives using biological evidence from the skeletons themselves. In this study we use dietary isotope analysis (δ13C and δ15N) of tissues which form at different points in the life course of an individual to create 'isotopic biographies' of goldrush-era individuals. In addition to telling the individual stories of these people, we also highlight differences in life experience between members of the European and Chinese communities, evidence for seasonal availability of resources on the goldfields, as well as unusual weaning patterns which potentially link to rural poverty experienced during childhood. • Isotopic evidence from multiple tissues allows detailed reconstruction of life stories from New Zealand's colonial period. • Isotopic reconstruction of diet on the goldfields suggests that Chinese and European populations retained distinct diets. • Reconstruction of weaning behaviour shows diversity in the childhood experiences of those who came to the goldfields. • Hair analysis shows potential seasonal resource use, periods of stress and even timing of movement to different areas. [ABSTRACT FROM AUTHOR]