1. Characterization of a new zeaxanthin producing strain of Chlorella saccharophila isolated from New Zealand marine waters.
- Author
-
Singh D, Puri M, Wilkens S, Mathur AS, Tuli DK, and Barrow CJ
- Subjects
- Base Sequence, DNA Primers, New Zealand, Polymerase Chain Reaction, Zeaxanthins, Chlorella metabolism, Seawater, Xanthophylls biosynthesis
- Abstract
A fast growing strain of Chlorella saccharophila was isolated from the marine water of New Zealand and grown in heterotrophic conditions using glucose or glycerol as a carbon source. Biomass production was found to be higher in culture fed with glucose (2.14±0.08 g L(-1)) as compared to glycerol (0.378±0.04 g L(-1)). Lipid accumulation was similar for both carbon sources, at approximately 22% of dry cell weight. However, carotenoid yield was higher with glycerol (0.406±0.0125 mg g(-1)) than with glucose (0.21±0.034 mg g(-1)). Further optimization of the growth of the isolate gave maximal carotenoid production of 16.39±1.19 mg g(-1) total carotenoid, containing 11.32±0.64 mg g(-1) zeaxanthin and 5.07±0.55 mg g(-1) β-carotene. Comparison of various chemical and physical carotenoid extraction methods showed that ultrasonication was required for maximum extraction yields. The new strain has potential for biofuel, with carotenoid co-production., (Crown Copyright © 2013. Published by Elsevier Ltd. All rights reserved.)
- Published
- 2013
- Full Text
- View/download PDF