1. Structural and functional analysis of surface proteins from an A(H3N8) influenza virus isolated from New England harbor seals.
- Author
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Yang H, Nguyen HT, Carney PJ, Guo Z, Chang JC, Jones J, Davis CT, Villanueva JM, Gubareva LV, and Stevens J
- Subjects
- Amino Acid Sequence, Animals, Antigens, Viral chemistry, Crystallography, X-Ray, Hemagglutinin Glycoproteins, Influenza Virus chemistry, Influenza A Virus, H3N8 Subtype chemistry, Influenza A Virus, H3N8 Subtype isolation & purification, Microbial Sensitivity Tests, Models, Molecular, Molecular Sequence Data, Neuraminidase chemistry, New England, Orthomyxoviridae Infections virology, Polysaccharides analysis, Protein Binding, Protein Conformation, Recombinant Proteins chemistry, Recombinant Proteins metabolism, Sequence Alignment, Viral Proteins chemistry, Antigens, Viral metabolism, Hemagglutinin Glycoproteins, Influenza Virus metabolism, Influenza A Virus, H3N8 Subtype physiology, Neuraminidase metabolism, Orthomyxoviridae Infections veterinary, Phoca virology, Viral Proteins metabolism, Virus Attachment
- Abstract
Unlabelled: In late 2011, an A(H3N8) influenza virus infection resulted in the deaths of 162 New England harbor seals. Virus sequence analysis and virus receptor binding studies highlighted potential markers responsible for mammalian adaptation and a mixed receptor binding preference (S. J. Anthony, J. A. St Leger, K. Pugliares, H. S. Ip, J. M. Chan, Z. W. Carpenter, I. Navarrete-Macias, M. Sanchez-Leon, J. T. Saliki, J. Pedersen, W. Karesh, P. Daszak, R. Rabadan, T. Rowles, W. I. Lipkin, MBio 3:e00166-00112, 2012, http://dx.doi.org/10.1128/mBio.00166-12). Here, we present a detailed structural and biochemical analysis of the surface antigens of the virus. Results obtained with recombinant proteins for both the hemagglutinin and neuraminidase indicate a true avian receptor binding preference. Although the detection of this virus in new species highlights an increased potential for cross-species transmission, our results indicate that the A(H3N8) virus currently poses a low risk to humans., Importance: Cross-species transmission of zoonotic influenza viruses increases public health concerns. Here, we report a molecular and structural study of the major surface proteins from an A(H3N8) influenza virus isolated from New England harbor seals. The results improve our understanding of these viruses as they evolve and provide important information to aid ongoing risk assessment analyses as these zoonotic influenza viruses continue to circulate and adapt to new hosts., (Copyright © 2015, American Society for Microbiology. All Rights Reserved.)
- Published
- 2015
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