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In recent years body composition studies on American Football players have focused on participants at the professional, NCAA Division I and NCAA Division III levels. Recent data utilizing skinfold and hydrostatic weighing to measure the body composition of NCAA Division II football players is currently not available. Purpose: The purpose of the present investigation was to examine the body composition of NCAA Division II football players utilizing both hydrostatic weighing and skinfold measurements. Methods: Sixty-eight NCAA Division II (DII) football players volunteered for this study. Body height (HT), body weight (BW), forced vital capacity and percent body fat were measured. Percent body fat was assessed across all playing positions: offensive line/tightends (OL/TE), defensive line (DL), offensive backs/receivers (OB/WR), defensive backs (DB), linebackers (LB) and quarterbacks/kickers (QB/K) through eight skinfold measurements (chest, abdominal, thigh, calf, subscapular, suprailliac, triceps & biceps) and hydrostatic weighing. Body density was calculated from skinfold measurements utilizing six regression equations with a pooled median value utilized for analysis. Percent body fat was calculated using the Schutte equation for African Americans and the Siri equation for Caucasian athletes. Results: Percent body fat calculated from skinfold measurements: OL/TE = 28.26 ( ± 1.19%) DL = 25.04 ( ± 2.82%) OB/WR = 15.12 ( ± 1.24%) DB = 15.40 ( ± 0.66%) LB = 18.63 ( ± 1.20%) QB/K = 17.86 ( ± 1.70%). Percent body fat calculated from hydrostatic weighing: OL/TE = 25.29 ( ± 1.42%) DL = 22.05 ( ± 3.21%) OB/WR = 11.98 ( ± 1.35%) DB = 12.76 ( ± 1.03%) LB = 14.99 ( ± 1.39%) QB/K = 14.53 ( ± 1.73%). Discussion: Upon inspection, these data show DII football players possessed a higher percentage of body fat when compared to their Division I (DI) counterparts published by Noel, Vanheest, Zaneteas & Rodgers (2003) (See table 1.). Practical Application: These data will allow strength and conditioning professionals and nutritionists to better monitor training and dietary programs of NCAA Division II football players.Table 1. Comparison of percentage of body fat between NCAA Division II football players and NCAA Division I football players. [ABSTRACT FROM AUTHOR]