1. Negeviruses isolated from mosquitoes in the Brazilian Amazon.
- Author
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da Silva Ribeiro, Ana Cláudia, Martins, Lívia Caricio, da Silva, Sandro Patroca, de Almeida Medeiros, Daniele Barbosa, Miranda, Keissy Karoline Pinheiro, Nunes Neto, Joaquim Pinto, de Oliveira Monteiro, Hamilton Antônio, do Nascimento, Bruna Lais Sena, Junior, Jose Wilson Rosa, Cruz, Ana Cecilia Ribeiro, da Costa Vasconcelos, Pedro Fernando, Carvalho, Valéria Lima, and Rodrigues, Sueli Guerreiro
- Subjects
PLANT viruses ,INSECT viruses ,NUCLEOTIDE sequencing ,BUNYAVIRUSES ,FLAVIVIRUSES - Abstract
Background: There are several groups of viruses including Insect Specific Viruses (ISV) such as the taxon Negevirus, a group of viruses phylogenetically related to plant viruses. Negeviruses replicate in mosquito cells, but not in vertebrate cells. Methods: Pools of hematophagous arthropods were inoculated in Vero and C6/36 cells. The cells were observed to detect possible cytopathic effect. Then, indirect immunofluorescence, RT-PCR, and nucleotide sequencing were performed. Results: Seven samples which presented negative results for flaviviruses, alphaviruses and bunyaviruses, but showed cytopathic effect in C6/36 cells were sequenced. We identified the occurrence of a variety of ISVs, most of them belonging to the taxon Negevirus: The Brejeira, Negev, Cordoba and Wallerfield viruses, including a new virus for science, tentatively named Feitosa virus. Conclusions: We detected negeviruses in the Amazon region, including two viruses that were isolated for the first time in Brazil: Cordoba virus and the Negev virus and, a new virus for science: the Feitosa virus. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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