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Sassoon docks is one of the oldest docks in Mumbai. It is one of the largest fish landing site in Mumbai. The dock gets untreated waste water and deteriorates the quality of seawater near the fish landing base. The dock is stench prominently emanated from the decayed fish and mishandled fish waste of shell fishes like prawns and shrimps. It is still active first wet docks. Heavy metals are found naturally which have high density and atomic weight. The heavy metal gets released through various agricultural industries, factories which cause severe effect on environment and as well as on organisms present in it. This can lead to multiple organ damage of organisms present in water. Heavy metals became strongly toxic as it gets mixed with water, soil. The microbial analysis of water can be determined through the present concentration of Escherichia coli, Coliforms. The bacterial count should not exceed more than 80 per 100 ml in sea water. The observed value is between 26 × 10³- 15 × 10³, 8.8 × 10³ _ 6.4 × 10³, 8.2 × 10³ _ 6.0 × 10³ for Total Bacterial Count, Coliforms, E.- Coli respectively. The bacterial count should be zero in any water. The observed value is much higher than the permissible limit which is due to the contamination and cause severe ill effects on marine organism and human as well as. The heavy metal get trapped in water due to release of industrial waste in the water. For different metals normal range differ from 0.001 to 0.1ml Excess presence of heavy metals deteriorate the various physiological processes. The Lead and Iron shows higher value i.e. 0.1 and 3.226, respectively. The heavy metals cause carcinogenic, tetragenic and mutagenic effects on organisms present in water. The three main source of dissolved iron sources are: sediments dissolution along continental margins, dust from the atmosphere and fluids from hydrothermal vents. High iron can accumulate in body of organism and can be fatal for them. [ABSTRACT FROM AUTHOR]