1. Polyfunctional Mycobacterium tuberculosis-specific effector memory CD4+ T cells at sites of pleural TB.
- Author
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El Fenniri L, Toossi Z, Aung H, El Iraki G, Bourkkadi J, Benamor J, Laskri A, Berrada N, Benjouad A, Mayanja-Kizza H, Betts MR, El Aouad R, and Canaday DH
- Subjects
- Adolescent, Adult, Aged, CD4-Positive T-Lymphocytes metabolism, Chemokine CCL3 biosynthesis, Female, Flow Cytometry, Humans, Interferon-gamma biosynthesis, Interleukin-2 biosynthesis, Male, Middle Aged, Morocco epidemiology, Mycobacterium tuberculosis cytology, Phenotype, Tuberculosis, Pleural epidemiology, Tuberculosis, Pleural genetics, Tumor Necrosis Factor-alpha biosynthesis, Uganda epidemiology, Young Adult, CD4-Positive T-Lymphocytes immunology, Mycobacterium tuberculosis immunology, Tuberculosis, Pleural immunology
- Abstract
Pleural tuberculosis (TB) is a common presentation of Mycobacterium tuberculosis (MTB) infection, and despite spontaneous resolution remains a strong risk factor for reactivation pulmonary TB in a majority of individuals. This study was undertaken to further understand the characteristics of immune cells at sites of pleural TB. A significant shift toward memory CD4+ T cells with an effector phenotype and away from naïve CD4+ T cells in pleural fluid as compared to blood mononuclear cells was found. These data suggest that effector T cells are capable of migrating to sites of active TB infection and/or the differentiation to effector phenotype T cells in situ is highly amplified. Using multi-parameter flow cytometry analysis, a significant portion of MTB-specific CD4+ T cells in the pleural space were polyfunctional demonstrating two, three or four simultaneous functions including IFN-gamma, IL-2, TNF-alpha, and or MIP-1 alpha production. A greater proportion of these polyfunctional cells were of effector memory rather than central memory phenotype. The role of these polyfunctional MTB-specific CD4+ T cells at sites of pleural TB requires further study., (Copyright © 2010 Elsevier Ltd. All rights reserved.)
- Published
- 2011
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