1. MirSNPs in clopidogrel metabolism genes predict cardiovascular disease risk: a case-control study and meta-analysis.
- Author
-
Sharma AR, Patagi S, Uk AR, Shetty R, Umakanth S, Satyamoorthy K, and Rai PS
- Subjects
- Aged, Aryldialkylphosphatase genetics, Asian People, Case-Control Studies, Collagen Type IV genetics, Cytochrome P-450 CYP3A genetics, Female, Genome-Wide Association Study, Genotype, Humans, Male, Middle Aged, Middle East, Mutation, Cardiovascular Diseases chemically induced, Cardiovascular Diseases genetics, Clopidogrel pharmacokinetics, MicroRNAs genetics, Platelet Aggregation Inhibitors pharmacokinetics, Polymorphism, Single Nucleotide genetics
- Abstract
Aim: The present study was conducted to decipher the inter-relationship of SNPs and miRNAs involved in pharmacogenomics of clopidogrel on predisposition to cardiovascular diseases (CVDs). Materials & methods: A case-control study was conducted on 410 cases and 386 controls to analyze the association of 13 mirSNPs on CVDs risk. Genotyping was performed by tetra-primer amplification refractory mutation system PCR and validated using Sanger DNA sequencing. miRNA expression analysis was performed using TaqMan assays. A meta-analysis was performed for PON1 rs662 with coronary artery disease. Results & conclusion: PON1 rs662, PON1 rs3917577, CYP3A5 rs15524, COL4A1 rs874204 and PTGIR rs1126510 polymorphisms showed association with CVDs. The miRNA hsa-miR-224-5p showed differential expression in the PON1 rs662 on South Asian and Middle East populations.PON1 rs662 on South Asian and Middle East populations.
- Published
- 2021
- Full Text
- View/download PDF