For the first time, we report the presence of Hannemania hylae (Ewing) parasitizing the long-footed robber frog Eleutherodactylus longipes (Baird) in a cave environment in Queretaro State, Mexico. A morphological comparison was performed between H. hylae and its closest species Hannemania bufonis Loomis and Welbourn and Hannemania monticola Welbourn and Loomis. The main characters to separate these species are the size of ocular plate, the length of some scutal setae, and the shape and length of some tarsal setae of the legs I and II. We inspected 47 frogs, the prevalence of infestation was 23.4%, mean abundance was 1.8 mites per host, mean intensity 7.9 mites per infested host, and the range intensity was 1-30 mites. Larvae of H. hylae were only collected during the dry season. A brief discussion is provided about the period of larval stage on the host and seasonality is compared with similar studies. [ABSTRACT FROM AUTHOR]