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Macrofungi represent one of the most difficult taxonomic groups of organisms to study and monitoring the diversity over time and space often requires a high field sampling effort. Two different monitoring approaches were used to assess macrofungal biodiversity in broadleaf Mediterranean forests in order to determine whether different sampling efforts can provide statistically similar diversity indices. Despite the different number of field visits, the results obtained show that some biodiversity indices (e.g. Shannon index, Eveness, Simpson index) values do not seem to be significantly affected by the survey approach chosen and a single survey per year has achieved comparable results to multiple surveys. This, in a wider biodiversity context it could produce a quick field methodology to study complex taxonomic groups, such as macrofungi. [ABSTRACT FROM AUTHOR]