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Galectins are a highly conserved family of β-galactoside binding lectins encoded by lectin, galactosidebinding soluble genes (LGALS). They are found on a variety of cells and tissues of vertebrates and invertebrates. Understanding the roles of galectins in host-pathogen interaction and activation of host innate, and adaptive immunity has become an important research question. Galectins are known to play a role in parasitic infection in small ruminants. However, little is known about LGALS variants in sheep and goat breeds across the world. In this study, we investigated galectin single nucleotide polymorphism (SNP) variants on five distinct sheep breeds from different geographical regions of Ethiopia. A total of 88 sheep (Arsi-Bale=12, Horro=24, Menz=18, Wolayita=18 and Blackhead Somali=16) were genotyped with Ovine Infinium HD SNP BeadChip (600K). An In Silico approach with Ensembl database (https://useast.ensembl.org/index. html) and SNP BeadChip (perfect matched reference SNP along with their genomic position) identified a total of nineteen SNPs from LGALS-1, -2, -3, -4, -7, -8, -9, -11 and -12 with a consequence of upstream, downstream, and missense gene variant. For all these variants, a highly variable minor allele frequency (MAF) was observed across five sheep populations. We also investigated a total of 308 Chinese goat (Qinggeli=48, Nanjiang=48, Luoping=48, Liaoning=116, and Guangfeng=48) genotyped with Illumina Goat 50K BeadChip. The goat chip only contained a SNP from LGal-4 (rs268252010) which was also found at variable MAF level across the said populations. These results have implications for animal health and production and warrant further investigation using targeted sequencing of galectin genes across different local and international sheep and goat breeds. [ABSTRACT FROM AUTHOR]