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This study presents a comprehensive investigation of the Paleocene coal in the Tirah area, District Khyber, Khyber Pakhtunkhwa, Pakistan, utilizing onsite Electrical Resistivity Tomography (ERT) and Ground Penetrating Radar (GPR) experiments combined with geochemical analyses of obtained coal-rock samples. ERT and GPR profiles, along with geochemical data, were collected and meticulously analyzed to assess the potential and quality of coal seams based on coal ranking, aiming to delineate their spatial distribution and future exploitation prospects. The study reveals a wide range of resistivity values, spanning from 8.93 Ωm to 2472 Ωm. The uppermost layer, comprising silt and clay with water saturation, exhibits resistivity values ranging from 8.93 Ωm to 50 Ωm. The subsequent stratum, characterized by wet sandstone with minor shale and clay, has resistivity values between 50 Ωm and 95.3 Ωm. Significantly, the zone with resistivity values between 95.3 Ωm and 800 Ωm is identified as the probable host of the coal seams. Based on the results, the coal seams are estimated to exist at depths ranging from 14 to 23 m, with resistivity values between 95.3 Ωm and 800 Ωm. GPR results further corroborate these findings, revealing coal-bearing strata at various depths, thus confirming the ERT results. Additionally, proximate and ultimate analysis provided insights into the quality of the coal, with average concentration values of moisture content (6.95%), ash content (22.12%), volatile matter (28.55%), fixed carbon (42.40%), carbon content (55.35%), hydrogen (4.7%), nitrogen (0.95%), sulfur (4.82%), and oxygen (10.06%). Comparative analyses of the obtained results with local coals from the Salt Range in Punjab, Pakistan, and coal from Ogboyaga, Nigeria, confirm that the studied coal ranks as sub-bituminous. This comprehensive assessment offers valuable insights into the potential and quality of Paleocene coal in the studied region and is applicable to other areas with similar geological settings. [ABSTRACT FROM AUTHOR]

Simple Summary: Theileria is the tick-borne disease-causing agent responsible for theileriosis in various animals. Like other tick-borne pathogens, the epidemiology, associated risk factors and genetic diversity of Theileria spp. have mostly remained unexplored in the region. The current study was designed to analyze the epidemiology, associated risk factors, and molecular characterization of Theileria spp. in the selected districts of Khyber Pakhtunkhwa, Pakistan. Among the chosen districts, the highest infection was found in Upper Dir, while the most prevalent species was Theileria annulata clustered with sequences for the same species reported from Pakistan, China, and Italy. The risk factors associated with Theileria infection included age, gender, breeds, feeding system, hygienic measure, farming system, stall system, and seasons. The microscopic examination, in combination with the molecular approach, will enhance the early diagnosis and accurate identification of Theileria spp. and facilitate effective control strategies against these parasites. Theileria annulata is a tick-associated parasite that causes tropical theileriosis in livestock and is responsible for huge economic losses. Studies have been neglected on the effect of Theileria spp. on cattle in Khyber Pakhtunkhwa (KP), Pakistan. The present study was designed to determine the genetic diversity and assess the risk factors associated with Theileria infection in selected districts of KP. Information on the risk factors related to the Theileria infection was collected through a questionnaire. Blood samples were collected from symptomatic cattle from January 2019 to February 2020, identified morphologically through microscopic examination, and processed for molecular characterization using the 18S rRNA gene as a genetic marker. Of the 555 cattle examined (136, 24.5%) and (294, 53%) were found positive for Theileria spp. by microscopic examination and a PCR test, respectively. Based on the PCR test, the highest prevalence of infection was found in district Upper Dir (46/75, 61.3%), followed by Lower Dir (54/90, 60%); Malakand (51/88, 57.9%); Peshawar (40/80, 50%); and Charsadda (52/112, 46.4%), with the lowest in Bajaur (51/110, 46.34%). A BLAST analysis of the 18S rDNA sequences showed 99.5% identity with T. annulata. In a phylogenetic tree, the 18S rDNA sequence of T. annulata clustered with sequences from Pakistan, China, and Italy. A significant association was observed between the prevalence of infection and different host characteristics. The highest infection was found in adult cattle (216/360, 60%); females (218/377, 57.8%); and Holstein Friesian (120/180, 66.6%). Theileria infection was significantly associated with management practices. Higher infection rates were observed in free-grazing cattle (190/412, 42.2%); those kept in unhygienic conditions (246/405, 60.7%); cattle in combined farming systems (165/255, 64.8%); and those in congested stall systems (150/218, 68.8%). Seasonal patterns were found to be significantly associated with infection, and a higher infection rate was observed in summer (215/350, 61.4%) than in winter (79/205, 38.5%). Identified risk factors should be considered in designing practical control approaches to reduce the burden of Theileria infection. Large scale studies are required to explore the diversity of Theileria species in KP, Pakistan. [ABSTRACT FROM AUTHOR]

Background: Hepatitis B Virus (HBV) is one of the most common human infectious agents, and the mutations in its genome may cause chronic hepatitis (CH), liver cirrhosis (LC), and hepatocellular carcinoma (HCC). This study was designed to characterize the enhancer-II (Enh-II) region of X gene in HBV positive patients to assess the association of such mutations with CH, LC, and HCC. Methods: HBV positive samples (N = 200) with patients' demographic and clinical data were collected from different regions of Khyber Pakhtunkhwa (KP), Pakistan. The Enh-II region of the HBx gene was sequenced and zanalyzed for polymorphism associated with advanced liver disease. Univariate and logistic regression analyses were performed to evaluate potent mutations associated with a risk for LC and HCC. Results: HBV Enh-II region sequences analysis revealed 25 different mutations. The highest frequency of mutations S101F (62.2%), A102V/R/G/I (56.25%), M103L/A (68.75%)were found in HCC, followed in LC and CH patients as 57.1%, 42.8%, 28.52% 16%, 15.2% and 18.4% respectively. H94 deletion in the α-box of the Enh-II region, associated with a high risk of HCC was found in half of the HCC patients. This deletion was present in 28.5% of LC and 6.5% of CH patients. Importantly, the high frequency of some notable mutations such as E109A/Y, A110S/K, Y111D/E, and F112L was first time reported in the entire study population. The frequencies of these mutations were high in HCC (43.75%, 37.5%, 50% and 43.75% respectively) as compared to LC (14.28%, 14.28%, 28.2% and 42.8%) and CH patients (12.8%, 15.2%, 16.8% and 16% respectively). Conclusion: Mutations associated with LC and HCC are prevalent in the Enh-II region in Pakistani HBV isolates. The mutations found are alarming in CH patients as these may progress to LC and HCC in a large number of patients. [ABSTRACT FROM AUTHOR]

The purpose of this research article was to investigate the impact of activity-oriented teaching on the students' performance in Physics at college level. An earlier study by Majid Khan, et. 2020, ten chapters from high school physics textbook were used. A sample of 60 students participated in that study. In the present study twenty (20) lessons were selected from 11th class Physics for the present study. All the science students at colleges of Khyber Pakhtunkhwa, studying Physics at the 11th grade, constituted the population. A sample of 80 students was randomly selected from Govt. College Peshawar, Mardan, Kohat and Malakand. Pre-test-Post-test Control Group Design? of experimental research was selected for this research study. Three MCQs type achievement tests were used as research tools for the data collection. Experimental group was taught with the help of activities whereas the control group was taught the same lessons through traditional method of teaching for the period of six (4) weeks. T-test was used to analyze the data. The results showed that the activity-based teaching is more effective for the development of higher order skills in the students. [ABSTRACT FROM AUTHOR]

The study was conducted in 2015-2016 in Tehsil Tangi, Charsadda District, Khyber Pakhtunkhwa. The aim was to analyse the poverty status of the Virginia Tobacco growers in the study area with Poverty Gap Index (PGI) method. The primary data was collected through structured questionnaire from 159 farmers. Monthly farmers' income was calculated from the total crop and non-crop income. The results revealed that the poverty ratio and Poverty Gap index were 8.3 percent and 0.016 percent for the Virginia tobacco growers and 42.52 percent and 0.076 percent for the non-tobacco growers, respectively. This shows that the Virginia Tobacco growers poverty status was better. Therefore, tobacco is recommended as cash crop in Charsadda District to reduce the poverty incidence. [ABSTRACT FROM AUTHOR]