1. New ceramide from the aerial part of Tinospora oblongifolia with cytotoxic activities.
- Author
-
Samita F, Ochieng CO, Owuor PO, and Manguro LA
- Subjects
- Antineoplastic Agents, Phytogenic chemistry, Camptothecin pharmacology, Ceramides chemistry, Drug Screening Assays, Antitumor, Humans, Inhibitory Concentration 50, KB Cells, Kenya, Molecular Structure, Plant Stems chemistry, Sitosterols chemistry, Sitosterols isolation & purification, Antineoplastic Agents, Phytogenic isolation & purification, Antineoplastic Agents, Phytogenic pharmacology, Ceramides isolation & purification, Ceramides pharmacology, Tinospora chemistry
- Abstract
A new ceramide, 2,3-dihydroxy-N-[(2S,3S,4R)-1,3,4-trihydroxyicosan-2-yl[tetracosanamide (1) along with four known compounds: 2-(4'-hydroxyphenyl)-ethyl lignocerate (2), docosyl-3,4-dihydroxy-trans-cinnamate (3), β-sitosterol (4) and β-sitosterol glycoside (5) were isolated from Tinospora oblongifolia (Menispermaceae). Their structures were determined on the basis of spectroscopic methods, mass spectrometry analysis as well as chemical transformation and by comparing their physical and spectral data with those reported in the literature. Compound 1 exhibited strong cytotoxic activity against KB cells with IC50 = 3.4 μM although less than that of camptothecin IC50 = 0.3 μM (positive control).
- Published
- 2014
- Full Text
- View/download PDF