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In 1929, it was reported that yellowing symptoms caused by a tobacco mosaic virus (TMV) yellow mosaic isolate were suppressed in tobacco plants that were systemically infected with a TMV light green isolate. Similar to vaccination, the phenomenon of cross-protection involves a whole plant being infected with an attenuated virus and involves the same or a closely related virus species. Therefore, attenuated viruses function as biological control agents. In Japan, many studies have been performed on cross-protection. For example, the tomato mosaic virus (ToMV)-L11A strain is an attenuated isolate developed by researchers and shows high control efficiency against wild-type ToMV in commercial tomato crops. Recently, an attenuated isolate of zucchini yellow mosaic virus (ZYMV)-2002 was developed and registered as a biological pesticide to control cucumber mosaic disease. In addition, attenuated isolates of pepper mild mottle virus (PMMoV), cucumber mosaic virus (CMV), tobacco mild green mosaic virus (TMGMV), melon yellow spot virus (MYSV), and watermelon mosaic virus (WMV) have been developed in Japan. These attenuated viruses, sometimes called plant vaccines, can be used not only as single vaccines but also as multiple vaccines. In this review, we provide an overview of studies on attenuated plant viruses developed in Japan. We also discuss the application of the attenuated strains, including the production of vaccinated seedlings. [ABSTRACT FROM AUTHOR]

Poaceae plant species, such as silver grass, are commonly used in mulching activities Japan. In contrast, local farmers have traditionally used Japanese knotweed (Fallopia japonica) mulch in the cultivation of solanaceous crops in the Nishi-Awa area of Japan, which is a Globally Important Agricultural Heritage Systems site. We have previously evaluated the positive effects of Japanese knotweed mulching on solanaceous crops, such as eggplants, tomato, and potato. In the present study, we observed that the naturally occurring diseases in the solanaceous crops tended to decrease when the knotweed mulching system was adopted, in comparison to when Poaceae mulch was adopted. In eggplants, leaf mold and powdery mildew decreased under Japanese knotweed mulching. We further evaluated the effects of Japanese knotweed mulching by inoculating test plants with Pseudomonas cichorii. We observed suppression of bacterial disease and tomato mosaic virus under Japanese knotweed mulching and following spraying with Japanese knotweed extracts. In addition, disease-resistance genes were expressed at high levels in Arabidopsis thaliana, a model plant, following treatment with Japanese knotweed extracts. The results suggest that Japanese knotweed has potential applications in future sustainable agriculture activities. [ABSTRACT FROM AUTHOR]

A tobamovirus, belonging to the P1 pathotype, was isolated from sweet pepper (Capsicum annuum) in Kochi Prefecture, Japan and was identified as the Paprika mild mottle virus Japanese strain (PaMMV-J). Unlike a reported PaMMV strain incapable of infecting tomato plants, PaMMV-J occasionally infected tomato plants, although the infectivity was lower than that of Tomato mosaic virus. Analysis of the distribution and replication of PaMMV-J in tomato using in vitro RNA transcripts from the cDNA clone of PaMMV-J suggest that the low infectivity of PaMMV-J to tomato plants is due to low levels of both replication and cell-to-cell movement. [ABSTRACT FROM AUTHOR]

Five human-specific markers were detected in 59-74% of 27 human fecal-source samples collected in Yamanashi Prefecture, Japan. Similarly, potential human-specific markers, crAssphage, pepper mild mottle virus (PMMoV), and tobacco mosaic virus were detected in 96-100% of samples, with crAssphage showing the maximum concentration of 12.03 log copies/L. However, these markers were detected in 100% (3/3) of pig fecal-source samples, suggesting their applicability as general fecal pollution markers. Microbial source tracking analysis demonstrated that the rivers are contaminated by human and pig fecal sources. CrAssphage showed higher marker concentrations in river water samples than PMMoV, suggesting the preference of crAssphage to PMMoV as a marker of fecal pollution.